GenBank-Updates@genbank.bio.net (02/14/91)
LOCUS TFETFFC2 5317 bp ds-DNA BCT 14-FEB-1991
DEFINITION Plasmid pTF-FC2 from Thiobacillus ferrooxidans mobA, mobB, mobC,
mobD, mobE genes, complete cds, ORF's 3, 5 and 8, complete cds, and
merR gene, 3' end.
ACCESSION M57717
KEYWORDS MobA; MobB; MobC; MobD; MobE; merR regulatory protein.
SOURCE Plasmid pTF-FC2 (from T.ferrooxidans) DNA.
ORGANISM Plasmid pTF-FC2
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 5317)
AUTHORS Rohrer,J. and Rawlings,D.E.
TITLE Sequence analysis and characterization of the mobilization region
of the broad-host-range plasmid, pTF-FC2, isolated from
Thiobacillus ferrooxidans
JOURNAL Unpublished (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS complement(<1..1227)
/note="ORF6"
/gene="mobA"
/codon_start=1227
CDS 2007..2363
/note="ORF1"
/gene="mobC"
/codon_start=2007
CDS 2376..3059
/note="ORF2"
/gene="mobD"
/codon_start=2376
CDS 3052..3693
/note="ORF4"
/gene="mobE"
/codon_start=3052
CDS 3678..4190
/note="ORF3"
/codon_start=3678
CDS 4187..4354
/note="ORF5"
/codon_start=4187
CDS 4585..4842
/note="ORF8"
/codon_start=4585
CDS 4951..5317
/product="merR regulatory protein"
/codon_start=4951
/partial
BASE COUNT 1118 a 1569 c 1553 g 1077 t
ORIGIN
1 atcgatgctt ttgcccttgt ccacgaaaga cacacccccg ccccgccccc cttcttcctt
61 ccggctgtaa tgcacctgct ggcccacgat ttcaggctga taggcgcgaa tgtcgcgcgg
121 cgtcggcggc tcgctgcggt cgccctcgat gcgctgcggc tcgctggccc ggtgtcgcca
181 ctgctcggca agctccgggc ttttctgcat ccgctgccac tgctccaggt cgggataggg
241 tctgaactgc tgccggtgct gctcgcgctg cttctggtgc ttctccttca aggcggcttt
301 ctctgcggcc tgctcggcgg caatcacgct gcgcatggcg ttgagcactt cgcccttgcc
361 tttccagttg ccgcgcatga gttcatcgcg gcgggccttc tgctgctcgg caagttgctt
421 gcgctcctgc tcctgccgct tgtcctgtac cagcttgtcc gcgttcttct ccgagtagtg
481 cgccttgcgc ccggtgatgt agtccttcca tcccggcacg tctggcttga tcggctccgg
541 ctcgcgctgt gctacctgct gacgctgcgg cggctggtaa gctccgagtc gcttttgcaa
601 cttggatagg ctcgcgtccc ggtctgcgct gctggccttc acgccaacat caccgacaaa
661 gagcgtcgcc ccgctgcctg ttttctcgta gcgcatcccc ttggctgcta actcgcggtg
721 caactgctcc cacgtctgcg cctttttgat gatcggtgcg ccgtcctcaa tggcgatgcg
781 ttcggcggat ttctccccgg tgcggttttc catgtcgcgc ttcggctggg ctggctggcg
841 cggcttgtcc ttgtcgatgt gctcccggcc taactcacca ttctccaaca cctgataacg
901 cccgttctgc tcacgctgcc agccttgtgc gttctcgatt cgtgctatcg ccttgtgcgc
961 tgcctcaatg tcgaagccgt tattgatctt caccaccttg agcgtttcag gatgcacgcg
1021 gttgatggca aggtgcaggt gcaagttgtc cgtgtcggcg tgcaaaccat agatggcttg
1081 atggtcttta acacctagct catccatgaa aatgcttacc gcttcttcga cctgctccgg
1141 gctgggctgc tcgccttctc gccaactcag gacatagtga ttgatcgtat ccttgcttcg
1201 cactgcctct tgtgacaagg caatcatttc tgccgtctgg ctctgtggtt catcggtgat
1261 gaatccacgc gccccggcat ggatgcactt ctcctgcgag ttctcacgct caggctcacg
1321 gatgtagcca gttaggcggg aaacgcgaac cgccttgctt gctgccttct gcgggttctt
1381 aaccttcttg acgatcacgg ctcagtttcc cgatataggc tttcaagacc acaagcgccc
1441 cggctgtgtc cttgctgtac acgcctccgc tctcgttgtg gatgtgctta agcaatccac
1501 cgatgcggcg caactctttg agcattaccg cgtcggcgct ggcaatgatc ggcctgccaa
1561 agtagcggcg gcggacaagc tccgacatgc tcaacccggc aaggtcggca tcctctttca
1621 agcgggcctt ctccgctgcg gtcagccgta cattgataac ggcgtccagc ggttccggcc
1681 cctgcgtttc aaatggcatc gtgtctcctt ctcgcggggg gcgggcgggg tgtgtcttgg
1741 acagtaagaa gcgagagcgc cgcactgtcc ggggtcaagg ggatacccct tgcaggataa
1801 gccagaccgt gagaagcgaa gcgccgaatg ggcaggcgtc cgttggaacc acgtggcgaa
1861 gccatgtgtg ttacaacggt catcctgtat tgctcaaccg ctctactatc atatcagcag
1921 tttaggcaca ctagatgcac tttgcaacaa gcataaaagg cgctatgctg tcaactccga
1981 aaccagtaag caaaggagtt agcagaatga aatacacgac agagcagcta gaggctatcg
2041 catcgaagct gcgtgatatg ccacaagtcg aaaaaaagaa gcaggagcac agcaagcaag
2101 aggccgtgcg cgtgctatca aaagagatcg cggctttgca aaagcgcgga tacacgcttg
2161 accagatttc ggaaacgctt cgcggtgaag gtctgagcat cgcaaccccg acactcaaga
2221 gctatttgca gagagcaaag ccaacgaaaa aagccccggt gcaagcgccg ggagacacac
2281 ccccgcccgc ccccgccgtc aagaagcctg cggatacatc gaaggcgaca tttaccccaa
2341 aacccgacag cgacgacatt taaggagtaa gcaacatggc aaaaagcatt tacctgatcg
2401 gcggcagcaa gggcggcgtt ggcaagtcgc ttgtcacgat ggcaacggtt gattacctgc
2461 aagaacgcgg cgaaagcgta ttgctgattg aatccgacac ctctaacccg gacgtgtgga
2521 aggcatacaa ggaaagcacg gaaacggagc ttatcaatct ggatgaggcg gacggatgga
2581 ttcagctagt gaacttgtgc gatagcaagc cggatagcgt cgtggtaatt aacgcggctg
2641 cgaggaataa caagggagtg agcgcctacg gtgaaacatt gaatagcaca cttgcggaac
2701 tcaagcgcaa gctggtcacg ctgtgggtga ttaaccgcca acgggatagc ctcgaactgc
2761 tcaaggaata catggatgca atcccgaacg cggatgttca tgtggtgcgc aatggacact
2821 tcggagagga aaagaaattc gagctttaca acggctcaaa gctgcgcacg gcagtagagg
2881 aacgcggcgg gcagtcggtg acattcccgg atatggccga ccgggtgagc gatgacattt
2941 acagcaagcg catgagcatt tcggtagcac tcaaggaact gccaattggc aaccgtgcgg
3001 aacttacccg gtggcgcaat gaggctaaaa aggtattgga gggcgtaatc catgagtgac
3061 cttgacgaca gctttgcaaa gctgctcggc aggcaaccca gtgacgcgga gcggcaaagc
3121 ctttaccgtg tgcgggatgc actcgggctg aaaaacaatg atgcgctctg gctggtgctc
3181 atggccttgc agcactacca aggccagtat gagaagttcc cgcaagcgat agcacaagca
3241 gccaaagata ccttggtcaa tttcaaggta acggcggatg caaccgtgaa agcatcagcg
3301 gaagcagcca aggcggattt agcgcaggcg gtagcagcag cagcgcaaga ggtcgcgcac
3361 aacacatctg caaagcagat gtggcagtgg gcggcgggct gcatcgccgt ggccttcctg
3421 tgcgtcggcc tgttcggctg gtacatgcac agcagcggca aggattccgg ctaccaagcc
3481 ggatacggcg cgggctacac ggaagccaag gacgagaaag cggcggcggc atgggcaaac
3541 acgccagaag gtcgaacggc ctaccgcttc gcccagtccg gcgaactgca acgccttgcc
3601 cggtgcagtg gcaagggctg gaaggtcgag aaaggcgctt gctaccccta cccagtagcg
3661 aatgagggca cttacggatg ggcgttgcca tgatcgacgc gctcaacatc gccacgatgc
3721 cgattgcaga caaggtgcat cggcacatgc tggcaagcta ctacgccttg caactggatg
3781 ccttgcaggc cgaagccaag cggctcggct acttcttcgc gcaagccgac acggcggcaa
3841 ccatgccgcc agtgctggcc gattgcttgg cgtgggcggt cgagtaccgg cgcaggtgct
3901 acctgtaccc gaattgcccg gaaagctggg aacgccacac cgccgacagc atgagcgacg
3961 gttacgccca agagcattgc cgctcgatgc tcgcggcgct ggccgcgctc ggcatccgtt
4021 tgcaggaagg ccagcaagcc tacgccctgc tggcggcgga ggaatgccgc caacgcgaga
4081 aagccagctt gccacccgag ccttcgccac tctcggaagt cgaagtgtcc agcttcgtcg
4141 atgagttttt caccaagctg gacgcaccca aggaggaagt cccgacatga gcacaaccgc
4201 caagatcgac tttgaaaccg gcctgttcga tgagggccag tacgaggaag cgccagacct
4261 gccagccatc cagccgaaac ggcggacaag tcgcgccccg ttcatcgtgg ccggtggctt
4321 gctgcttggg gtcgtctcag aaaacggaaa ttaaagcacg ctaaggcacg acaagcgtgc
4381 gcatatgctg cttcacggac tgaaggggct gttttcctgt tcccggtgcg ggcgatccgg
4441 gcgttgcgcg aaaccgcata acccgctcag ttagtacttg acgttgaagt aagggccaag
4501 gtctatcgta atgacgcgcg gcgctcatgg cttctccggc cgggagttct cgcgcacctg
4561 accacacttt tttggagaaa taccatgact accaaacgca aagtcgaagt tttcagcgcg
4621 ggatgcccct cctgccagac cgccattgaa ctcgtcaatc gcctcgcatg cggttcgtgc
4681 gaggtctcaa tcctcgatat gaacgacatc aatgtggcca aacgcgcccg tgatctcggt
4741 gttcggtcgg tgccggcggt cgccatcaac ggccagctgg cttcgtgctg ctccggcagc
4801 ggcatcgagg aacaggcgct tcgctggcct cggcaagcct aggcctcccc atcggcacgc
4861 gcgtgcctct cgctggaagc tcgcgattaa tttcttaggc ggaactcttg accatggtgt
4921 tcactccaaa gtttatatta acacttacaa atggaggaca ccatgcacac catcggcaag
4981 gtggcaacgc tagccggagt cagcaccgat acgctgcgct actatgaaaa agagcacctg
5041 atcgaacctg catctagaac cgctgcgggc taccggctgt acaacgatga agcggtgcgg
5101 cgcatccgtt ttatcaaaac cgctcagcac tgcggattct cgttgtcgga cattcaggag
5161 ttgctgacgc tgaagcgaac tgatagtgcc tgttgcgagg atgtgcgcag cgtggccatc
5221 gagaagaggc tgcgcatcgc gcgcaaacta cgggtcttgc aggccatgtc ggccgccctc
5281 aatgacttga tccagagttg cgaaggcggt gctgcag
//GenBank-Updates@genbank.bio.net (02/23/91)
LOCUS TFETFFC2 5317 bp ds-DNA BCT 22-FEB-1991
DEFINITION Plasmid pTF-FC2 from Thiobacillus ferrooxidans mobA, mobB, mobC,
mobD, mobE genes, complete cds, ORF's 3, 5 and 8, complete cds, and
merR gene, 3' end.
ACCESSION M57717
KEYWORDS MobA; MobB; MobC; MobD; MobE; merR regulatory protein.
SOURCE Plasmid pTF-FC2 (from T.ferrooxidans) DNA.
ORGANISM Plasmid pTF-FC2
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 5317)
AUTHORS Rohrer,J. and Rawlings,D.E.
TITLE Sequence analysis and characterization of the mobilization region
of the broad-host-range plasmid, pTF-FC2, isolated from
Thiobacillus ferrooxidans
JOURNAL Unpublished (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS complement(<1..1227)
/note="ORF6"
/gene="mobA"
/codon_start=1227
CDS 2007..2363
/note="ORF1"
/gene="mobC"
/codon_start=2007
CDS 2376..3059
/note="ORF2"
/gene="mobD"
/codon_start=2376
CDS 3052..3693
/note="ORF4"
/gene="mobE"
/codon_start=3052
CDS 3678..4190
/note="ORF3"
/codon_start=3678
CDS 4187..4354
/note="ORF5"
/codon_start=4187
CDS 4585..4842
/note="ORF8"
/codon_start=4585
CDS 4951..5317
/product="merR regulatory protein"
/codon_start=4951
/partial
BASE COUNT 1118 a 1569 c 1553 g 1077 t
ORIGIN
1 atcgatgctt ttgcccttgt ccacgaaaga cacacccccg ccccgccccc cttcttcctt
61 ccggctgtaa tgcacctgct ggcccacgat ttcaggctga taggcgcgaa tgtcgcgcgg
121 cgtcggcggc tcgctgcggt cgccctcgat gcgctgcggc tcgctggccc ggtgtcgcca
181 ctgctcggca agctccgggc ttttctgcat ccgctgccac tgctccaggt cgggataggg
241 tctgaactgc tgccggtgct gctcgcgctg cttctggtgc ttctccttca aggcggcttt
301 ctctgcggcc tgctcggcgg caatcacgct gcgcatggcg ttgagcactt cgcccttgcc
361 tttccagttg ccgcgcatga gttcatcgcg gcgggccttc tgctgctcgg caagttgctt
421 gcgctcctgc tcctgccgct tgtcctgtac cagcttgtcc gcgttcttct ccgagtagtg
481 cgccttgcgc ccggtgatgt agtccttcca tcccggcacg tctggcttga tcggctccgg
541 ctcgcgctgt gctacctgct gacgctgcgg cggctggtaa gctccgagtc gcttttgcaa
601 cttggatagg ctcgcgtccc ggtctgcgct gctggccttc acgccaacat caccgacaaa
661 gagcgtcgcc ccgctgcctg ttttctcgta gcgcatcccc ttggctgcta actcgcggtg
721 caactgctcc cacgtctgcg cctttttgat gatcggtgcg ccgtcctcaa tggcgatgcg
781 ttcggcggat ttctccccgg tgcggttttc catgtcgcgc ttcggctggg ctggctggcg
841 cggcttgtcc ttgtcgatgt gctcccggcc taactcacca ttctccaaca cctgataacg
901 cccgttctgc tcacgctgcc agccttgtgc gttctcgatt cgtgctatcg ccttgtgcgc
961 tgcctcaatg tcgaagccgt tattgatctt caccaccttg agcgtttcag gatgcacgcg
1021 gttgatggca aggtgcaggt gcaagttgtc cgtgtcggcg tgcaaaccat agatggcttg
1081 atggtcttta acacctagct catccatgaa aatgcttacc gcttcttcga cctgctccgg
1141 gctgggctgc tcgccttctc gccaactcag gacatagtga ttgatcgtat ccttgcttcg
1201 cactgcctct tgtgacaagg caatcatttc tgccgtctgg ctctgtggtt catcggtgat
1261 gaatccacgc gccccggcat ggatgcactt ctcctgcgag ttctcacgct caggctcacg
1321 gatgtagcca gttaggcggg aaacgcgaac cgccttgctt gctgccttct gcgggttctt
1381 aaccttcttg acgatcacgg ctcagtttcc cgatataggc tttcaagacc acaagcgccc
1441 cggctgtgtc cttgctgtac acgcctccgc tctcgttgtg gatgtgctta agcaatccac
1501 cgatgcggcg caactctttg agcattaccg cgtcggcgct ggcaatgatc ggcctgccaa
1561 agtagcggcg gcggacaagc tccgacatgc tcaacccggc aaggtcggca tcctctttca
1621 agcgggcctt ctccgctgcg gtcagccgta cattgataac ggcgtccagc ggttccggcc
1681 cctgcgtttc aaatggcatc gtgtctcctt ctcgcggggg gcgggcgggg tgtgtcttgg
1741 acagtaagaa gcgagagcgc cgcactgtcc ggggtcaagg ggatacccct tgcaggataa
1801 gccagaccgt gagaagcgaa gcgccgaatg ggcaggcgtc cgttggaacc acgtggcgaa
1861 gccatgtgtg ttacaacggt catcctgtat tgctcaaccg ctctactatc atatcagcag
1921 tttaggcaca ctagatgcac tttgcaacaa gcataaaagg cgctatgctg tcaactccga
1981 aaccagtaag caaaggagtt agcagaatga aatacacgac agagcagcta gaggctatcg
2041 catcgaagct gcgtgatatg ccacaagtcg aaaaaaagaa gcaggagcac agcaagcaag
2101 aggccgtgcg cgtgctatca aaagagatcg cggctttgca aaagcgcgga tacacgcttg
2161 accagatttc ggaaacgctt cgcggtgaag gtctgagcat cgcaaccccg acactcaaga
2221 gctatttgca gagagcaaag ccaacgaaaa aagccccggt gcaagcgccg ggagacacac
2281 ccccgcccgc ccccgccgtc aagaagcctg cggatacatc gaaggcgaca tttaccccaa
2341 aacccgacag cgacgacatt taaggagtaa gcaacatggc aaaaagcatt tacctgatcg
2401 gcggcagcaa gggcggcgtt ggcaagtcgc ttgtcacgat ggcaacggtt gattacctgc
2461 aagaacgcgg cgaaagcgta ttgctgattg aatccgacac ctctaacccg gacgtgtgga
2521 aggcatacaa ggaaagcacg gaaacggagc ttatcaatct ggatgaggcg gacggatgga
2581 ttcagctagt gaacttgtgc gatagcaagc cggatagcgt cgtggtaatt aacgcggctg
2641 cgaggaataa caagggagtg agcgcctacg gtgaaacatt gaatagcaca cttgcggaac
2701 tcaagcgcaa gctggtcacg ctgtgggtga ttaaccgcca acgggatagc ctcgaactgc
2761 tcaaggaata catggatgca atcccgaacg cggatgttca tgtggtgcgc aatggacact
2821 tcggagagga aaagaaattc gagctttaca acggctcaaa gctgcgcacg gcagtagagg
2881 aacgcggcgg gcagtcggtg acattcccgg atatggccga ccgggtgagc gatgacattt
2941 acagcaagcg catgagcatt tcggtagcac tcaaggaact gccaattggc aaccgtgcgg
3001 aacttacccg gtggcgcaat gaggctaaaa aggtattgga gggcgtaatc catgagtgac
3061 cttgacgaca gctttgcaaa gctgctcggc aggcaaccca gtgacgcgga gcggcaaagc
3121 ctttaccgtg tgcgggatgc actcgggctg aaaaacaatg atgcgctctg gctggtgctc
3181 atggccttgc agcactacca aggccagtat gagaagttcc cgcaagcgat agcacaagca
3241 gccaaagata ccttggtcaa tttcaaggta acggcggatg caaccgtgaa agcatcagcg
3301 gaagcagcca aggcggattt agcgcaggcg gtagcagcag cagcgcaaga ggtcgcgcac
3361 aacacatctg caaagcagat gtggcagtgg gcggcgggct gcatcgccgt ggccttcctg
3421 tgcgtcggcc tgttcggctg gtacatgcac agcagcggca aggattccgg ctaccaagcc
3481 ggatacggcg cgggctacac ggaagccaag gacgagaaag cggcggcggc atgggcaaac
3541 acgccagaag gtcgaacggc ctaccgcttc gcccagtccg gcgaactgca acgccttgcc
3601 cggtgcagtg gcaagggctg gaaggtcgag aaaggcgctt gctaccccta cccagtagcg
3661 aatgagggca cttacggatg ggcgttgcca tgatcgacgc gctcaacatc gccacgatgc
3721 cgattgcaga caaggtgcat cggcacatgc tggcaagcta ctacgccttg caactggatg
3781 ccttgcaggc cgaagccaag cggctcggct acttcttcgc gcaagccgac acggcggcaa
3841 ccatgccgcc agtgctggcc gattgcttgg cgtgggcggt cgagtaccgg cgcaggtgct
3901 acctgtaccc gaattgcccg gaaagctggg aacgccacac cgccgacagc atgagcgacg
3961 gttacgccca agagcattgc cgctcgatgc tcgcggcgct ggccgcgctc ggcatccgtt
4021 tgcaggaagg ccagcaagcc tacgccctgc tggcggcgga ggaatgccgc caacgcgaga
4081 aagccagctt gccacccgag ccttcgccac tctcggaagt cgaagtgtcc agcttcgtcg
4141 atgagttttt caccaagctg gacgcaccca aggaggaagt cccgacatga gcacaaccgc
4201 caagatcgac tttgaaaccg gcctgttcga tgagggccag tacgaggaag cgccagacct
4261 gccagccatc cagccgaaac ggcggacaag tcgcgccccg ttcatcgtgg ccggtggctt
4321 gctgcttggg gtcgtctcag aaaacggaaa ttaaagcacg ctaaggcacg acaagcgtgc
4381 gcatatgctg cttcacggac tgaaggggct gttttcctgt tcccggtgcg ggcgatccgg
4441 gcgttgcgcg aaaccgcata acccgctcag ttagtacttg acgttgaagt aagggccaag
4501 gtctatcgta atgacgcgcg gcgctcatgg cttctccggc cgggagttct cgcgcacctg
4561 accacacttt tttggagaaa taccatgact accaaacgca aagtcgaagt tttcagcgcg
4621 ggatgcccct cctgccagac cgccattgaa ctcgtcaatc gcctcgcatg cggttcgtgc
4681 gaggtctcaa tcctcgatat gaacgacatc aatgtggcca aacgcgcccg tgatctcggt
4741 gttcggtcgg tgccggcggt cgccatcaac ggccagctgg cttcgtgctg ctccggcagc
4801 ggcatcgagg aacaggcgct tcgctggcct cggcaagcct aggcctcccc atcggcacgc
4861 gcgtgcctct cgctggaagc tcgcgattaa tttcttaggc ggaactcttg accatggtgt
4921 tcactccaaa gtttatatta acacttacaa atggaggaca ccatgcacac catcggcaag
4981 gtggcaacgc tagccggagt cagcaccgat acgctgcgct actatgaaaa agagcacctg
5041 atcgaacctg catctagaac cgctgcgggc taccggctgt acaacgatga agcggtgcgg
5101 cgcatccgtt ttatcaaaac cgctcagcac tgcggattct cgttgtcgga cattcaggag
5161 ttgctgacgc tgaagcgaac tgatagtgcc tgttgcgagg atgtgcgcag cgtggccatc
5221 gagaagaggc tgcgcatcgc gcgcaaacta cgggtcttgc aggccatgtc ggccgccctc
5281 aatgacttga tccagagttg cgaaggcggt gctgcag
//