GenBank-Updates@genbank.bio.net (02/23/91)
LOCUS R48FOL 1618 bp DNA BCT 23-FEB-1991
DEFINITION Plasmid R483 trimethropin-resistant (Dhfr) gene.
ACCESSION K00052
KEYWORDS dihydrofolate reductase; drug resistance; plasmid; trimethoprim
resistance.
SOURCE Plasmid R483 (from E.coli) DNA.
ORGANISM Plasmid R483
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 1618)
AUTHORS Simonsen,C.C., Chen,E.Y. and Levinson,A.D.
TITLE Identification of the type I trimethoprim-resistant dihydrofolate
reductase specified by the Escherichia coli R-plasmid R483:
Comparison with procaryotic and eucaryotic dihydrofolate reductases
JOURNAL J. Bacteriol. 155, 1001-1008 (1983)
STANDARD simple staff_review
FEATURES Location/Qualifiers
CDS 986..1444
/EC_number="1.5.1.3"
/product="dihydrofolate reductase"
/gene="Dhfr"
/codon_start=986
BASE COUNT 478 a 317 c 356 g 467 t
ORIGIN
1 tcgataagca gaagggtatt tgtgatctaa agcaaaaggc agcgatggcc ctacgccttg
61 taagttgtcg tcttgctgaa taagccgcgc ttgctcaatg agttatttta ttgctgggat
121 taggcgcgtg ggcagtaggc tgtttctgct tttcccaccc ttaccgtcat gcacagtgat
181 gcagccatta tcaaaatcaa aatctttaac ccgcaaacgc aagcattcat taatgcgcaa
241 acctgcacca tacagcagcg taaaaataac ttggttgcga gtatccataa cctgcaaaat
301 gcgttgcact tcatttgcag agataacaga gggtagccgt ctaggcttgc ttgcagggat
361 ataatcaata tcgcccaacg gctgttgtaa aaacctgttg tacaaaaaag ctagggcatt
421 taaagcgatt ttctgcgtgt ttatggctac atgtctgctg tttgctaagc tggataaaaa
481 cagcctgacc tcttcactgc ccatggtctg aggatgacgt tttttgtgaa acagaataaa
541 acgcttaatc cagtgcaggt aagttttttc agttttcagc gcataacctt tttgtcgcat
601 atccgtgcgt atagaattta aaaatggact gttagacata aaacgctcct tgtcttgcaa
661 ctgtctgcct atacagccta ttctagctgg gatttaaaaa agtgcctgtt ttttacgcct
721 agagatgctt gtttaccggt agagttttaa tttaatgcta aataaattaa aatgttatga
781 gttctttggg tgagataatg tgcatcgtgc aagcaggata gacggcatgc acgatttgta
841 ataacagagt gtcttgtatt tttaaagaaa gtctatttaa tacaagtgat tatattaatt
901 aacggtaagc atcagcgggt gacaaaacga gcatgcttac taataaaatg ttaacctctg
961 aggaagaatt gtgaaactat cactaatggt agctatatcg aagaatggag ttatcgggaa
1021 tggccctgat attccatgga gtgccaaagg tgaacagctc ctgtttaaag ctattaccta
1081 taaccaatgg ctgttggttg gacgcaagac ttttgaatca atgggagcat tacccaaccg
1141 aaagtatgcg gtcgtaacac gttcaagttt tacatctgac aatgagaacg tattgatctt
1201 tccatcaatt aaagatgctt taaccaacct aaagaaaata acggatcatg tcattgtttc
1261 aggtggtggg gagatataca aaagcctgat cgatcaagta gatacactac atatatctac
1321 aatagacatc gagccggaag gtgatgttta ctttcctgaa atccccagca attttaggcc
1381 agtttttacc caagacttcg cctctaacat aaattatagt taccaaatct ggcaaaaggg
1441 ttaacaagtg gcagcaacgg attcgcaaac ctgtcacgcc ttttgtgcca aaagccgcgc
1501 caggtttgcg atccgctgtg ccaggcgtta ggcgtcatat gaagatttcg gtgatccctg
1561 agcaggtggc ggaaacattg gatgctgaga accatttcat tgttcgtgaa gtgttcga
//