GenBank-Updates@genbank.bio.net (02/23/91)
LOCUS BSUCWLAA 3003 bp ds-DNA BCT 23-FEB-1991
DEFINITION Bacillus subtilis N-acetylmuramoyl-L-alanine amidase (cwlA) gene,
complete cds.
ACCESSION M59232
KEYWORDS N-Acetylmuramoyl-L-alanine amidase; autolysin.
SOURCE Bacillus subtilis (strain 168) DNA, clone Lambda PE3.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 3003)
AUTHORS Foster,S.J.
TITLE Cloning, expression, sequence analysis and biochemical
characterisation of an autolytic amidase from Bacillus subtilis 168
JOURNAL Unpublished (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 1..501
/note="open reading frame 1"
/codon_start=1
/partial
CDS 546..968
/note="open reading frame 2"
/codon_start=546
CDS 1013..1831
/EC_number="3.5.1.28"
/product="N-Acetylmuramoyl-L-alanine amidase"
/gene="cwlA"
/codon_start=1013
CDS 1996..2475
/note="open reading frame 4"
/codon_start=1996
CDS 2491..2853
/note="open reading frame 5"
/codon_start=2491
/partial
BASE COUNT 1018 a 490 c 635 g 860 t
ORIGIN
1 gaattcttac tttaccagtc attgggcgca ggtgaagagg atataaacat taaccatact
61 gttcctatta cagcagttgc tccgttctca gtccagttag ataaaagcgg gttaactaat
121 gatggtcgtt taaaagttca aactgaaggc ttgaatctta gctcattaga cactcaatca
181 aaaacaatgg atattgtctt tcacgataaa acagaaacca taggtgaggg taacccattc
241 accgttggat cattcaaaac gttgctcatt gaggtttatg ggacggctga gacaagtgaa
301 ttgaagttct ggggtaaatc cttatcggga acaaaaagag cccttagagg gcagaaagtg
361 gatgacggaa cgtttgccac tagcacaaaa ggaaaatcag aagcttggtc ttttagcatt
421 actggcttta aagaaattgt tatggagctt acagctttaa caaatggaaa cttttcagtt
481 agagggacgg ccgtctcata agatccggct gtcctttttt atttgcctcg gaggaggtga
541 ttagaatgga ggagacaagt ttgtttatca attttgaaac attagattta gcaagagtat
601 atttatttgg aggggtgaag taccttgatt tacttctagt acttagcata attgacgttt
661 taacaggagt aatcaaggca tggaaattca aaaaactgcg aagtcgaagc gcatggtttg
721 gctatgtccg caagctactc aatttctttg cggtcatttt agcaaacgtg attgatacag
781 tactcaattt gaacggtgtc ttaacctttg gtaccgttct tttttatatc gctaatgaag
841 gtttgtcaat aactgaaaac ttagcacaga ttggtgttaa aataccatca tcaataacag
901 atcgattaca aacaattgag aacgaaaaag aacagagtaa gaataacgca gacaaagctg
961 ctggctaagc cagtggcttt ttttatctta ctgacagaag gagagagggt atatggccat
1021 taaagttgta aagaatctag tctctaaatc aaagtatgga ttgaaatgtc ctaatccaat
1081 gaaagctgaa tatatcacta ttcataacac tgcgaatgat gcttcagcag ccaatgagat
1141 ttcttacatg aagaataact ctagctcaac aagttttcac tttgcagtag acgataaaca
1201 agtcattcaa ggtattccaa cgaatcgtaa cgcttggcac acaggagatg gaacaaacgg
1261 tacagggaat cgcaagtcta ttggtgtcga aatttgttat agcaagtcag gaggggtacg
1321 atataaggca gcggaaaagc ttgctattaa gtttgtggct cagctactta aagaacgtgg
1381 atggggtatt gatcgagtcc gcaaacatca agactggaat ggtaagtatt gcccgcaccg
1441 cattttgtca gagggaagat ggattcaagt taagactgca attgaagcag aattgaaaaa
1501 gttgggcgga aaaacaaact caagcaaagc aagtgtagct aaaaagaaaa caacaaacac
1561 aagcagcaaa aaaacgtcat atgcgctacc atccggtatt tttaaagtga agagcccaat
1621 gatgagaggg gaaaaggtaa cacaaattca aaaagcactg gctgcactat acttttaccc
1681 ggataaagga gcaaaaaaca acggcattga cggcgtgtat ggtccgaaaa cagcagatgc
1741 aattagacga ttccagtcta tgtacgggct tactcaagac ggtatttacg gaccaaaaac
1801 gaaagcgaaa cttgaagctc tcttgaagta aataaatagt ctccttgagt atctttctca
1861 aggagatact tatgtttatt ttgttacaga tagttcaaaa ttattctaat atttgagtaa
1921 aaagtattat ttttctagtc tgaacataat gttatcattt acatgtaaat aatttaaaag
1981 aaagggattg atttcatgtt taagaaatta cttttagcaa catctgcatt aacattctct
2041 ttatcattag ttcttccgtt ggatggacat gccaaagctc aagaggtaac attacaggca
2101 cagcaggaag tcacttatca aactcccgtt aaactatccg aattaccaac taatacaact
2161 gaacaatctg gtgaatttca cacaaatggt attaaaaaat ggattgctaa agaagcaatg
2221 aaggcaacag ccagtgcgct tcgacatggt gggagaattg ttggtgaggt agtcgatgag
2281 ttaggtggat cagcaggaaa aacattcgca aaacatactg atgatgtagc agatgctttg
2341 gacgagttag ttaaacgagg tgacgttgtg gaggacgcaa ttattgacac ggtttcctca
2401 tatctaattg atgctggtgt aaaatcttct acagctagaa ctattgcaag tgtatttact
2461 ttcttagctt tttaattgag gtgatttgaa ttggtaacag tagaagaaag gcttgacaac
2521 ctagaaaaaa aagtcgagaa gcaggctttt caactaaggt tagtccaaca attagcagct
2581 gattatgatc ggtttggctt atttgatcaa gttttggcct atgatttaag tgaaaaacag
2641 tatcaagaat taagagagtt aacgagccag tacactgata agattaaaaa tggcgaagag
2701 gtctctcttc ataactttac tgaagaattt aaaagaattt tgaaagatat tgaaaaagaa
2761 gtggatttcg aaaagtttat ttctctctgg ttaaagggac cagaagaagg ttttggattt
2821 tctaaagctt tacataacca tttctttaat taaagataag gaccagccac ataataagct
2881 ggtctttctt tattttccct caagttcgaa ctcctatgaa cagaatacca ggtatctatc
2941 cttccaaata tattgttatt agcttgaggg tttgtgtgtt tttcttcatt aatcatagaa
3001 ttc
//