GenBank-Updates@genbank.bio.net (02/23/91)
LOCUS FPLSSIA 134 bp ds-DNA BCT 23-FEB-1991
DEFINITION E.coli F plasmid ssiA gene.
ACCESSION D90179
KEYWORDS DNA replication; primosome assembly site; single-strand initiation
sequence; ssiA gene.
SOURCE Escherichia coli (strain K12) plasmid F factor.
ORGANISM Plasmid F
Prokaryota; Bacteria.
REFERENCE 1 (sites)
AUTHORS Murotsu,T., Tsutsui,H. and Matsubara,K.
TITLE Identification of the minimal essential region for the replication
origin of miniF plasmid
JOURNAL Mol. Gen. Genet. 196, 373-378 (1984)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 134)
AUTHORS Nomura,N., Masai,H., Inuzuka,M., Miyazaki,C., Ohtsubo,E., Itoh,T.,
Sasamoto,S., Matsui,M., Ishizaki,R. and Arai,K.-.
TITLE Identification of eleven single-strand initiation sequences for
priming of DNA replication in F, R6K, R100 and ColE2 plasmids
JOURNAL Unpublished (1991)
STANDARD full staff_entry
COMMENT
[Mol. Gen. Genet. 196, 373-378 (1984)] sites.
These data kindly submitted in computer readable form by:
Nobuo Nomura
Molecular Oncology Laboratory
Nippon Veterinary and Zootechnical College
1-396 Kosugicho, Nakahara-ku
Kawasaki, Kanagawa 211
Japan
Phone: 81-44-733-5230
Fax: 81-44-733-5192
FEATURES Location/Qualifiers
misc_feature 17..106
/note="ssiA(F.f5)"
repeat_region 24..77
/note="inverted repeat"
variation 25..25
/note="t in [Gene (1990) In press]; deletion in [Mol. Gen.
Genet. 196, 373-378 (1984)]"
variation 46..47
/note="ag in [Gene (1990) In press]; deletion in [Mol.
Gen. Genet. 196, 373-378 (1984)]"
BASE COUNT 34 a 37 c 35 g 28 t
ORIGIN
1 ggtctgtcct tttacagcca gtagtgctcg ccgcagtcga gcgacagggc gaagccctcg
61 agtgagcgag gaagcaccag ggaacagcac ttatatattc tgcttacaca cgatgcctga
121 aaaaacttcc cttg
//