GenBank-Updates@genbank.bio.net (02/23/91)
LOCUS FPLSSIBC 228 bp ds-DNA BCT 23-FEB-1991
DEFINITION E.coli F plasmid ssiB and ssiC gene.
ACCESSION D90177
KEYWORDS DNA replication; primosome assembly site; single-strand initiation
sequence; ssiB gene; ssiC gene.
SOURCE Escherichia coli (strain K12) plasmid F factor.
ORGANISM Plasmid F
Prokaryota; Bacteria.
REFERENCE 1 (sites)
AUTHORS Jalajakumari,M.B., Guidolin,A., Buhj,H.J. and Manning,P.A.
TITLE Surface exclusion genes traS and traT of the F sex factor of
Escherichia coli K-12 - Determination of the nucleotide sequence
and promoter and terminator activities
JOURNAL J. Mol. Biol. 198, 1-11 (1987)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 228)
AUTHORS Nomura,N., Masai,H., Inuzuka,M., Miyazaki,C., Ohtsubo,E., Itoh,T.,
Sasamoto,S., Matsui,M., Ishizaki,R. and Arai,K.-.
TITLE Identification of eleven single-strand initiation sequences for
priming of DNA replication in F, R6K, R100 and ColE2 plasmids
JOURNAL Unpublished (1991)
STANDARD full staff_entry
COMMENT
[J. Mol. Biol. 198, 1-11 (1987)] sites.
These data kindly submitted in computer readable form by:
Nobuo Nomura
Molecular Oncology Laboratory
Nippon Veterinary and Zootechnical College
1-396 Kosugicho, Nakahara-ku
Kawasaki, Kanagawa 211
Japan
Phone: 81-44-733-5230
Fax: 81-44-733-5192
FEATURES Location/Qualifiers
CDS <1..33
/note="traT (C-terminal)"
variation 41..41
/note="c in [Gene (1990) In press]; deletion in [J. Mol.
Biol. 198, 1-11 (1987)]"
variation 44..45
/note="ga in [Gene (1990) In press]; ag in [J. Mol. Biol.
198, 1-11 (1987)]"
variation 56..56
/note="c in [Gene (1990) In press]; deletion in [J. Mol.
Biol. 198, 1-11 (1987)]"
misc_feature 62..151
/note="ssiC (F.f2b)"
repeat_region 94..137
/note="inverted repeat"
misc_feature 132..227
/note="ssiB (F.f2a)"
variation 195..195
/note="g in [Gene (1990) In press]; deletion in [J. Mol.
Biol. 198, 1-11 (1987)]"
variation 200..203
/note="gccg in [Gene (1990) In press]; deletion in [J.
Mol. Biol. 198, 1-11 (1987)]"
BASE COUNT 58 a 56 c 50 g 64 t
ORIGIN
1 cagctggcca aatcaatcgc aaatattctc tgattatctt ccggagctgg tctgaccggc
61 ctcctgactg acgcctcgct ttgctcgttg tccaaccccg gaaagaaata aaataaattt
121 tatttctccc cggcgtttcc gaaataaata tgaaaacact gagcggttgt gcaaattctt
181 tgtgcgaccg tgaagtgttg ccggcgaagc cggaatttaa cggttatt
//