GenBank-Updates@genbank.bio.net (02/23/91)
LOCUS PSECAMABA 1950 bp ds-DNA BCT 23-FEB-1991
DEFINITION Pseudomonas putida, NADH-putidaredoxin reductase (camA) gene,
putidaredoxin (camB) gene and cytochrome P-450cam (camC) gene.
ACCESSION D00528
KEYWORDS camphor; cytochrome P-450cam.
SOURCE Pseudomonas putida ATCC17453 (strain PpG1), total cellular DNA,
clone pJP45.
ORGANISM Pseudomonas putida
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Pseudomonadaceae.
REFERENCE 1 (bases 1 to 1950)
AUTHORS Koga,H., Yamaguchi,E., Matsunaga,K., Aramaki,H. and Horiuchi,T.
TITLE Cloning and nucleotide sequences of NADH-putidaredoxin reductase
gene (camA) and putidaredoxin gene (camB) involved in cytochrome
P-450cam hydroxylase of Pseudomonas putida
JOURNAL J. Biochem. 106, 831-836 (1989)
STANDARD full staff_entry
COMMENT Submitted in computer readable form by H. Koga on 13-Jan-1990.
FEATURES Location/Qualifiers
CDS <1..93
/note="cytochrome P-450cam (camC), carboxy terminus"
misc_signal 101..104
/note="SD sequence for camA"
CDS 115..1383
/note="NADH-putidaredoxin reductase (camA)"
misc_signal 1427..1430
/note="SD sequence for camB"
CDS 1439..1762
/note="putidaredoxin (camB)"
mat_peptide 1442..1759
/note="putidaredoxin mature peptide"
BASE COUNT 450 a 555 c 575 g 370 t
ORIGIN 2 bp upstream of SmaI site.
1 ccgggtgccc agattcagca caagagcggc atcgtcagcg gcgtgcaggc actccctctg
61 gtctgggatc cggcgactac caaagcggta taaacacatg ggagtgcgtg ctaagtgaac
121 gcaaacgaca acgtggtcat cgtcggtacc ggactggctg gcgttgaggt cgccttcggc
181 ctgcgcgcca gcggctggga aggcaatatc cggttggtgg gggatgcgac ggtaattccc
241 catcacctac caccgctatc caaagcttac ttggccggca aagccacagc ggaaagcctg
301 tacctgagaa ccccagatgc ctatgcagcg cagaacatcc aactactcgg aggcacacag
361 gtaacggcta tcaaccgcga ccgacagcaa gtaatcctat cggatggccg ggcactggat
421 tacgaccggc tggtattggc taccggaggg cgtccaagac ccctaccggt ggccagtggc
481 gcagttggaa aggcgaacaa ctttcgatac ctgcgcacac tcgaggacgc cgagtgcatt
541 cgccggcagc tgattgcgga taaccgtctg gtggtgattg gtggcggcta cattggcctt
601 gaagtggctg ccaccgccat caaggcgaac atgcacgtca ccctgcttga tacggcagcc
661 cgggttctgg agcgggttac cgccccgccg gtatcggcct tttacgagca cctacaccgc
721 gaagccggcg ttgacatacg aaccggcacg caggtgtgcg ggttcgagat gtcgaccgac
781 caacagaagg ttaccgccgt cctctgcgag gacggcacaa ggctgccagc ggatctggta
841 atcgccggga ttggcctgat accaaactgc gagttggcca gtgcggccgg cctgcaggtt
901 gataacggca tcgtgatcaa cgaacacatg cagacctctg atcccttgat catggccgtc
961 ggcgactgtg cccgatttca cagtcagctc tatgaccgct gggtgcgtat cgaatcggtg
1021 cccaatgcct tggagcaggc acgaaagatc gccgccatcc tctgtggcaa ggtgccacgc
1081 gatgaggcgg cgccctggtt ctggtccgat cagtatgaga tcggattgaa gatggtcgga
1141 ctgtccgaag ggtacgaccg gatcattgtc cgcggctctt tggcgcaacc cgacttcagc
1201 gttttctacc tgcagggaga ccgggtattg gcggtcgata cagtgaaccg tccagtggag
1261 ttcaaccagt caaaacaaat aatcacggat cgtttgccgg ttgaaccaaa cctactcggt
1321 gacgaaagcg tgccgttaaa ggaaatcatc gccgccgcca aagctgaact gagtagtgcc
1381 tgaaatctat acccacaata aatcaccgtt ttgccccata gcgtgtgagg ataaacagat
1441 gtctaaagta gtgtatgtgt cacatgatgg aacgcgtcgc gaactggatg tggcggatgg
1501 cgtcagcctg atgcaggctg cagtctccaa tggtatctac gatattgtcg gtgattgtgg
1561 cggcagcgcc agctgtgcca cctgccatgt ctatgtgaac gaagcgttca cggacaaggt
1621 gcccgccgcc aacgagcggg aaatcggcat gctggagtgc gtcacggccg aactgaagcc
1681 gaacagcagg ctctgctgcc agatcatcat gacgcccgag ctggatggca tcgtggtcga
1741 tgttcccgat aggcaatggt aaaccacaat ggtaaaccac tgcgagccaa aacagccgag
1801 caggagcgca gtccggcaac accttattaa gcacatgccg aaccctattt gcagcgcttc
1861 atgcctgcaa agtcccgatt gatgaaatcc gggctccaag caaggagccc ggaatctctc
1921 accgccacga aatcaatggc caatcccggg
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