GenBank-Updates@genbank.bio.net (02/23/91)
LOCUS POVG 4973 bp ss-DNA VRL 23-FEB-1991
DEFINITION Porcine parvovirus genome, almost complete sequence.
ACCESSION D00623
KEYWORDS capsid proteins; non-structural protein.
SOURCE Porcine parvovirus (strain NADL-2), RF DNA from infected swine
testis cells, clone pPPV-10.
ORGANISM Porcine parvovirus
Viridae; ss-DNA nonenveloped viruses; Parvoviridae; Parvovirus.
REFERENCE 1 (bases 1 to 4973)
AUTHORS Ranz,A.I., Manclus,J.J., Diaz-zroca,E. and Casal,J.I.
TITLE Porcine parvovirus: DNA sequence and genome organization
JOURNAL J. Gen. Virol. 70, 2541-2553 (1989)
STANDARD full staff_entry
COMMENT Submitted in computer readable form by Casal,J.I. on 12-FEB-1990.
The sequence is complete except for approx. 80 nt at the 5' end.
FEATURES Location/Qualifiers
CDS join(2268..2295,2383..4526)
/note="capsid protein VP1"
misc_signal 183..188
/note="promoter consensus sequence"
CDS 279..2261
/note="non-structural protein NS1"
misc_signal 1923..1927
/note="promoter consensus sequence"
misc_signal 2260..2266
/note="5' donor splice junction for VP2 mRNA (putative)"
exon 2268..2295
/note="capsid protein VP1, exon 1 (putative)"
misc_signal 2293..2302
/note="5' donor splice junction for VP1 mRNA (putative)"
misc_signal 2374..2382
/note="3' acceptor splice junction for VP2 and VP1 mRNAs"
exon 2383..4526
/note="capsid protein VP1, exon 2 (putative)"
CDS 2787..4526
/note="capsid protein VP2 (putative)"
misc_signal 4540..4545
/note="polyadenylation signal"
misc_signal 4592..4597
/note="polyadenylation signal"
misc_signal 4598..4603
/note="polyadenylation signal"
misc_signal 4614..4619
/note="polyadenylation signal"
misc_signal 4667..4672
/note="polyadenylation signal"
misc_signal 4719..4724
/note="polyadenylation signal"
misc_signal 4725..4730
/note="polyadenylation signal"
misc_signal 4741..4746
/note="polyadenylation signal"
BASE COUNT 1964 a 1010 c 861 g 1138 t
ORIGIN near 5' end of viral RNA.
1 ctttaaactg accaactgtc tttgcgtatg gtgacgtgtg acgcgcgtcc ttcggcagtc
61 acacgtcacc atcagcaaag acagttggtc cagtttaaag attaataaga caattccatt
121 ggctgaaaag aggcgggaaa ttcaaaaaaa gaggcgggaa aaaaagaggt ggagcctaac
181 actataaata cagttgctta cttcagttag ttcctttctg cttcagactg cacttcgctc
241 cagagacaca gctacaaact actctcagct actgcagcat ggcagcggga aacacttact
301 cggaagaggt actaaaagct accaactggc ttcaagataa tgctcaaaaa gaagcattct
361 cttatgtatt taaaacacaa aaagtcaatc taaatggaaa agaaattgct tggaataact
421 acaacaaaga tacaacagat gcggaaatga taaacctaca aagaggagca gaaacatcat
481 gggaccaggc aacagacatg gaatgggaat cagaaatcga cagcctcaca aaaggccaag
541 tactgatttt tgactctctt gttaaaaaat gtctctttga aggtatattg caaaagaacc
601 taagtccaag tgactgctac tggttcatac agcatgaaca tggtcaagat actggctatc
661 actgccatgt actactaggt ggaaaaggct tacaacaagc aatgggaaaa tggttcagaa
721 aacaattaaa caatttatgg agtagatggt taataatgca atgcaaagta cctctaacac
781 cagttgaaag aataaaatta agggaattag cagaggatgg tgagtgggta tcgctactaa
841 cctacactca caaacaaact aaaaaacaat atacaaaaat gactcatttt ggaaatatga
901 ttgcttacta cttcctaaat aaaaaaagaa agacaactga aagagagcat ggatattatc
961 tcagctcaga ttctggcttc atgacaaatt tcttaaaaga aggcgagaga cacttagtca
1021 gtcacctatt tactgaagca aataaacctg aaactgtgga aacaacggtt actacagctc
1081 aggaagccaa aagaggcaaa atacaaacaa aaaaagaagt aagcataaaa tgcacaataa
1141 gagacttggt taataaaaga tgtactagca tagaagactg gatgatgaca gatccagaca
1201 gttatataga aatgatggct caaaccggag gagaaaattt aatcaaaaat acactagaaa
1261 taacaactct tactctagca agaacaaaaa cagcatatga cttaatactt gaaaaggcaa
1321 aaccaagcat gctaccaaca tttaatatta gcaatacaag aacatgtaaa atattcagca
1381 tgcacaattg gaactacatt aaatgctgcc atgctataac ttgtgtacta aacagacaag
1441 gaggaaaaag aaatacaatt ctatttcatg ggccagcatc aacaggaaaa agtataattg
1501 ctcaacacat tgcaaactta gttggtaatg ttggttgcta caatgcagcc aatgtgaact
1561 ttccatttaa tgactgtaca aataaaaact taatatggat tgaagaagca ggaaacttct
1621 ctaaccaagt aaaccaattc aaagccatat gttcaggtca aacaattaga attgaccaaa
1681 aaggtaaagg aagcaaacaa attgaaccaa ctcctgtaat aatgactaca aatgaagaca
1741 taactaaagt tagaatagga tgcgaggaaa gaccagaaca tacacaacca ataagagaca
1801 gaatgttaaa cataaaccta accagaaaac tgccaggtga ttttggactt ttagaagaaa
1861 ctgaatggcc actaatatgt gcttggttgg taaagaaagg ttaccaagca acaatggcta
1921 gctatatgca tcattgggga aatgtacctg attggtcaga aaaatgggag gagccaaaaa
1981 tgcaaacccc aataaataca ccaacagact ctcagatttc cacatcagtg aaaacttcgc
2041 cagcggacaa caactacgca gcaactccaa tacaggagga cctggattta gctttagcct
2101 tggagccgtg gagcgagcca acaacaccaa ctttcacaac tgcattaact caacacgcca
2161 gattcagcaa tacggacaca agtccaactt ggtcggaaat agaaaccgac ataagagcct
2221 gctttggtga aaactgtgca cccacaacaa accttgaata aggtaggatg gcgcctcctg
2281 caaaaagagc aagaggtaag ggtagtttta agggggtggt ggcatacata taaaactaac
2341 tgcaaataat ttttttatat attacaggac taactctacc aggtacaata cttggtccag
2401 gaaactcact agaccaagga gaaccaacta atccatcaga cgccgcagca aaagaacacg
2461 acgaagccta cgacaaatac ataaaatctg gaaaaaatcc tacattctac ttctcagcag
2521 ctgatgaaaa attcataaaa gaaactgaac acgcaaaaga ctacggaggt aaaattggac
2581 attacttctt cagagcaaag cgtgcctttg ctccaaaact ctcagaaaca gactcaccaa
2641 ctacatctca acaaccagag gtaagaagat cgccgagaaa acacccaggg tctaaaccac
2701 caggaaaaag acctgctcca agacatattt ttataaactt agctaaaaaa aaagctaaag
2761 ggacatctaa tacaaactct aactcaatga gtgaaaatgt ggaacaacac aaccctatta
2821 atgcagcgac tgaattgtct gcaacaggaa atgaatctgg gggtgggggc ggcggtggcg
2881 ggggtagggg tgctgggggg gttggtgtgt ctacaggtag tttcaataat caaacagaat
2941 ttcaatactt gggggagggc ttggttagaa tcactgcaca cgcatcaaga ctcatacatc
3001 taaatatgcc agaacacgaa acatacaaaa gaatacatgt actaaattca gaatcagggt
3061 cggcgggaca aatggtacaa gacgatgcac acacacaaat ggtaacacct tggtcactaa
3121 tagatgctaa cgcatgggga gtgtggttca atccagcgga ctggcagtta atatccaaca
3181 acatgacaga aataaactta gttagttttg aacaagcaat attcaatgta gtacttaaaa
3241 caattacaga atcagcaacc tcaccaccaa ccaaaatata taataatgat ctaactgcaa
3301 gcttaatggt cgcactagac accaataaca cacttccata cacaccagca gcacctagaa
3361 gtgaaacact tggtttttat ccatggttac ctacaaaacc aactcaatac agatattacc
3421 tatcatgcat cagaaaccta aatccaccaa catacactgg acaatcacaa ccaaataaca
3481 gactcaatac aaacaggcta cacagtgaca ttatgttcta cacaatagaa aatgcagtac
3541 caattcatct tctaagaaca ggagatgaat tctccacagg aatatatcac tttgacacaa
3601 aaccactaaa attaactcac tcatggcaaa caaacagatc tctaggactg cctccaaaac
3661 tactaactga acctaccaca gaaggagacc aacacccagg aacactacca gcagctaaca
3721 caagaaaagg ttatcaccaa acaattaata atagctacac agaagcaaca gcaattaggc
3781 cagctcaggt aggatataat acaccataca tgaattttga atactccaat ggtggaccat
3841 ttctaactcc tatagtacca acagcagaca cacaatataa tgatgatgaa ccaaatggtg
3901 ctataagatt tacaatggat taccaacatg gacacttaac cacatcttca caagagctag
3961 aaagatacac attcaatcca caaagtaaat gtggaagagc tccaaagcaa caatttaatc
4021 aacaggcacc actaaaccta gaaaatacaa ataatggaac acttttacct tcagatccaa
4081 taggagggaa atctaacatg catttcatga atacactcaa tacatatgga ccattaacag
4141 cactaaacaa tactgcacct gtatttccaa atggtcaaat atgggataaa gaacttgata
4201 cagatctaaa acctagacta catgttacag ctccatttgt ttgtaaaaac aatccaccag
4261 gacaactatt tgtaaaaata gcaccaaacc taacagatga tttcaatgct gactctcctc
4321 aacaacctag aataataact tattcaaact tttggtggaa aggaacacta acattcacag
4381 caaaaatgag atccagtaat atgtggaacc ctattcaaca acacacaaca acagcagaaa
4441 acattggtaa atatattcct acaaatattg gtggcataaa aatgtttcca gaatattcac
4501 aacttatacc aagaaaatta tactagaaat aactctgtaa ataaaaactc agttacttgg
4561 ttaatcatgt actactatca ttgtatactt caataaaaat aaattgtaaa atcaataaaa
4621 ctaagttact tagtttctgt atacctatac tagaaataac tctgtaaata aaaactcagt
4681 tacttggtta atcatgtact actatcattg tatacttcaa taaaaataaa ttgtaaaatc
4741 aataaaacta agttacttag tttctgtata ccaattatcc ccaaaaaaca ataaaatttt
4801 aaaaagaaac aagctctcat gtgtttacta ttaactaaac caaccacact tatatgacct
4861 tatgtcttta gggtggtggt ggaattacta tgtattcctt tgagttagtt ggtcgccccg
4921 tatgctaaac cagcgacgag agccgcttgg ttatgcccca aaggcgacca acg
//