GenBank-Updates@genbank.bio.net (02/23/91)
LOCUS CSTC1TOX 4592 bp ds-DNA PHG 23-FEB-1991
DEFINITION Bacteriophage c-st (from C.botulinum) C1-tox gene for botulinum C1
neurotoxin.
ACCESSION D90210
KEYWORDS C1 toxin; neurotoxin.
SOURCE Bacteriophage c-st (from C.botulinum type C-Stockholm) DNA.
ORGANISM Bacteriophage c-st
Viridae; Nonclassified viruses.
REFERENCE 1 (bases 1 to 4592)
AUTHORS Kimura,K., Fujii,N., Tsuzuki,K., Murakami,T., Indoh,T.,
Yokosawa,N., Takeshi,K., Syuto,B. and Oguma,K.
TITLE The complete nucleotide sequence of the gene coding for botulinum
type C1 toxin in the c-st phage genome
JOURNAL Biochem. Biophys. Res. Commun. (1990) In press
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Kouichi Kimura
Department of Microbiology
Sapporo Medical College
1 South, 17 West
Sapporo 060
Japan
E-mail: nfujii%niguts.nig.ac.jp@vax2.nlm.nih.gov
Phone: 011-611-2111 x2334
Fax: 011-612-5861
FEATURES Location/Qualifiers
misc_signal 255..260
/note="-35 region"
misc_signal 293..298
/note="-10 region"
misc_signal 305..310
/note="SD sequence"
CDS 321..4196
/note="ORF for C1 toxin"
BASE COUNT 1869 a 460 c 714 g 1549 t
ORIGIN
1 aataaatata gatgaaagta aagtatatgt acaaaagtgg gatgagtgta taatttgtgt
61 attagacggt acagaaaaat atttagatat atctcctgaa aataatagaa tacaattagt
121 aagttccaaa gataatgcaa aaaagattac agttaatact gatttattta gacctgattg
181 tataacattt tcatataatg ataaatattt ttctctatca cttagagatg gagattataa
241 ttggatgata tgtaatgaca ataacaaggt gctaaaggtg cacatttgtg gatattagaa
301 agttaggaga tgttagtatt atgccaataa caattaacaa ctttaattat tcagatcctg
361 ttgataataa aaatatttta tatttagata ctcatttaaa tacactagct aatgagcctg
421 aaaaagcctt tcgcattaca ggaaatatat gggtaatacc tgatagattt tcaagaaatt
481 ctaatccaaa tttaaataaa cctcctcgag ttacaagccc taaaagtggt tattatgatc
541 ctaattattt gagtactgat tctgacaaag atacattttt aaaagaaatt ataaagttat
601 ttaaaagaat taattctaga gaaataggag aagaattaat atatagactt tcgacagata
661 taccctttcc tgggaataac aatactccaa ttaatacttt tgattttgat gtagatttta
721 acagtgttga tgttaaaact agacaaggta acaactgggt taaaactggt agcataaatc
781 ctagtgttat aataactgga cctagagaaa acattataga tccagaaact tctacgttta
841 aattaactaa caatactttt gcggcacaag aaggatttgg tgctttatca ataatttcaa
901 tatcacctag atttatgcta acatatagta atgcaactaa tgatgtagga gagggtagat
961 tttctaagtc tgaattttgc atggatccaa tactaatttt aatgcatgaa cttaatcatg
1021 caatgcataa tttatatgga atagctatac caaatgatca aacaatttca tctgtaacta
1081 gtaatatttt ttattctcaa tataatgtga aattagagta tgcagaaata tatgcatttg
1141 gaggtccaac tatagacctt attcctaaaa gtgcaaggaa atattttgag gaaaaggcat
1201 tggattatta tagatctata gctaaaagac ttaatagtat aactactgca aatccttcaa
1261 gctttaataa atatataggg gaatataaac agaaacttat tagaaagtat agattcgtag
1321 tagaatcttc aggtgaagtt acagtaaatc gtaataagtt tgttgagtta tataatgaac
1381 ttacacaaat atttacagaa tttaactacg ctaaaatata taatgtacaa aataggaaaa
1441 tatatctttc aaatgtatat actccggtta cggcgaatat attagacgat aatgtttatg
1501 atatacaaaa tggatttaat atacctaaaa gtaatttaaa tgtactattt atgggtcaaa
1561 atttatctcg aaatccagca ttaagaaaag tcaatcctga aaatatgctt tatttattta
1621 caaaattttg tcataaagca atagatggta gatcattata taataaaaca ttagattgta
1681 gagagctttt agttaaaaat actgacttac cctttatagg tgatattagt gatgttaaaa
1741 ctgatatatt tttaagaaaa gatattaatg aagaaactga agttatatac tatccggaca
1801 atgtttcagt agatcaagtt attctcagta agaatacctc agaacatgga caactagatt
1861 tattataccc tagtattgac agtgagagtg aaatattacc aggggagaat caagtctttt
1921 atgataatag aactcaaaat gttgattatt tgaattctta ttattaccta gaatctcaaa
1981 aactaagtga taatgttgaa gattttactt ttacgagatc aattgaggag gctttggata
2041 atagtgcaaa agtatatact tactttccta cactagctaa taaagtaaat gcgggtgttc
2101 aaggtggttt atttttaatg tgggcaaatg atgtagttga agattttact acaaatattc
2161 taagaaaaga tacattagat aaaatatcag atgtatcagc tattattccc tatataggac
2221 ccgcattaaa tataagtaat tctgtaagaa gaggaaattt tactgaagca tttgcagtta
2281 ctggtgtaac tattttatta gaagcatttc ctgaatttac aatacctgca cttggtgcat
2341 ttgtgattta tagtaaggtt caagaaagaa acgagattat taaaactata gataattgtt
2401 tagaacaaag gattaagaga tggaaagatt catatgaatg gatgatggga acgtggttat
2461 ccaggattat tactcaattt aataatataa gttatcaaat gtatgattct ttaaattatc
2521 aggcaggtgc aatcaaagct aaaatagatt tagaatataa aaaatattca ggaagtgata
2581 aagaaaatat aaaaagtcaa gttgaaaatt taaaaaatag tttagatgta aaaatttcgg
2641 aagcaatgaa taatataaat aaatttatac gagaatgttc cgtaacatat ttatttaaaa
2701 atatgttacc taaagtaatt gatgaattaa atgagtttga tcgaaatact aaagcaaaat
2761 taattaatct tatagatagt cataatatta ttctagttgg tgaagtagat aaattaaaag
2821 caaaagtaaa taatagcttt caaaatacaa taccctttaa tattttttca tatactaata
2881 attctttatt aaaagatata attaatgaat atttcaataa tattaatgat tcaaaaattt
2941 tgagcctaca aaacagaaaa aatactttag tggatacatc aggatataat gcagaagtga
3001 gtgaagaagg cgatgttcag cttaatccaa tatttccatt tgactttaaa ttaggtagtt
3061 caggggagga tagaggtaaa gttatagtaa cccagaatga aaatattgta tataattcta
3121 tgtatgaaag ttttagcatt agtttttgga ttagaataaa taaatgggta agtaatttac
3181 ctggatatac tataattgat agtgttaaaa ataactcagg ttggagtata ggtattatta
3241 gtaatttttt agtatttact ttaaaacaaa atgaagatag tgaacaaagt ataaatttta
3301 gttatgatat atcaaataat gctcctggat acaataaatg gttttttgta actgttacta
3361 acaatatgat gggaaatatg aagatttata taaatggaaa attaatagat actataaaag
3421 ttaaagaact aactggaatt aattttagca aaactataac atttgaaata aataaaattc
3481 cagataccgg tttgattact tcagattctg ataacatcaa tatgtggata agagattttt
3541 atatatttgc taaagaatta gatggtaaag atattaatat attatttaat agcttgcaat
3601 atactaatgt tgtaaaagat tattggggaa atgatttaag atataataaa gaatattata
3661 tggttaatat agattattta aatagatata tgtatgcgaa ctcacgacaa attgttttta
3721 atacacgtag aaataataat gacttcaatg aaggatataa aattataata aaaagaatca
3781 gaggaaatac aaatgatact agagtacgag gaggagatat tttatatttt gatatgacaa
3841 ttaataacaa agcatataat ttgtttatga agaatgaaac tatgtatgca gataatcata
3901 gtactgaaga tatatatgct ataggtttaa gagaacaaac aaaggatata aatgataata
3961 ttatatttca aatacaacca atgaataata cttattatta cgcatctcaa atatttaaat
4021 caaattttaa tggagaaaat atttctggaa tatgttcaat aggtacttat cgttttagac
4081 ttggaggtga ttggtataga cacaattatt tggtgcctac tgtgaagcaa ggaaattatg
4141 cttcattatt agaatcaaca tcaactcatt ggggttttgt acctgtaagt gaataaataa
4201 tgattaataa tataaattat gttaaatatt ttaatattaa gttgaaatat gttttgatta
4261 gtcgaacatt aataaaataa ctaaataaat tatatttaaa actgttaaaa tagtatattt
4321 attatcaatt acagtagtaa aatactgatt gctctgtcta taatcttcca gagttaaaaa
4381 agaagatgtt taatttttag aatttattag gtaaaagaga tacagttagc gcactaaaaa
4441 attaaattaa attattttaa aaagttattg acattagaaa agtatggtag tatacattat
4501 aaatgtggaa aggagataaa gcaaataaat cttagtacaa aattaaatta gaaaatatta
4561 aaggaggatt aatatggaac acgatcaaga tt
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