GenBank-Updates@genbank.bio.net (02/28/91)
LOCUS ECOMCRBC 2705 bp ds-DNA BCT 28-FEB-1991
DEFINITION E.coli mcrB and mcrC genes, complete cds.
ACCESSION M58752
KEYWORDS mcrB gene; mcrC gene.
SOURCE E.coli K-12 DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 2705)
AUTHORS Dila,D., Sutherland,E., Moran,L., Slatko,B. and Raleigh,E.
TITLE Genetic and sequence organization of the mcrBC locus of Escherichia
coli K-12
JOURNAL J. Bacteriol. 172, 4888-4900 (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 129..1526
/function="Mediates sequence-specific restriction of
cytosine-modified DNA."
/product="McrB protein"
/gene="mcrB"
/codon_start=129
CDS 1496..2572
/function="Modifies the specificity of McrB restriction by
expanding the range of modified sequences restricted."
/product="McrC protein"
/gene="mcrC"
/codon_start=1496
terminator 2573..2598
/gene="mcrC"
terminator 2595..2621
/gene="mcrC"
terminator 2632..2647
/gene="mcrC"
BASE COUNT 848 a 559 c 522 g 776 t
ORIGIN
1 gttaaccacc gggagccttc cactgactca atagaaactt tccccctcag taaatattta
61 ccagtctgat tttgcagtaa aaatctattg tttcagtacg ttgcgaaagc gataatagag
121 gcttagcaat gaggaaggca tatcttatgg aatctattca accctggatt gaaaaattta
181 ttaagcaagc acagcaacaa cgttcgcaat ccactaaaga ttatccaacg tcttaccgta
241 acctgcgagt aaaattgagt ttcggttatg gtaattttac gtctattccc tggtttgcat
301 ttcttggaga aggtcaggaa gcttctaacg gtatatatcc cgttattctc tattataaag
361 attttgatga gttggttttg gcttatggta taagcgacac gaatgaacca catgcccaat
421 ggcagttctc ttcagacata cctaaaacaa tcgcagagta ttttcaggca acttcgggtg
481 tatatcctaa aaaatacgga cagtcctatt acgcctgttc ccaaaaagtc tcacagggta
541 ttgattacac ccgatttgcc tctatgctgg acaacataat caacgactat aaattaatat
601 ttaattctgg caagagtgtt attccaccta tgtcaaaaac tgaatcatac tgtctggaag
661 atgcgttaaa tgatttgttt atccctgaaa ccacaataga gacgatactc aaacgattaa
721 ccatcaaaaa aaatattatc ctccaggggc cgcccggcgt tggaaaaacc tttgttgcac
781 gccgtctggc ttacttgctg acaggagaaa aggctccgca acgcgtcaat atggttcagt
841 tccatcaatc ttatagctat gaggatttta tacagggcta tcgtccgaat ggcgtcggct
901 tccgacgtaa agacggcata ttttacaatt tttgtcagca agctaaagag cagccagaga
961 aaaagtatat ttttattata gatgaaatca atcgtgccaa tctcagtaaa gtatttggcg
1021 aagtgatgat gttaatggaa catgataaac gaggtgaaaa ctggtctgtt cccctaacct
1081 actccgaaaa cgatgaagaa cgattctatg tcccggagaa tgtttatatc atcggtttaa
1141 tgaatactgc cgatcgctct ctggccgttg ttgactatgc cctacgcaga cgattttctt
1201 tcatagatat tgagccaggt tttgatacac cacagttccg gaatttttta ctgaataaaa
1261 aagcagaacc ttcatttgtt gagtctttat gccaaaaaat gaacgagttg aaccaggaaa
1321 tcagcaaaga ggccactatc cttgggaaag gattccgcat tgggcatagt tacttctgct
1381 gtgggttgga agatggcacc tctccggata cgcaatggct taatgaaatt gtgatgacgg
1441 atatcgcccc tttactcgaa gaatatttct ttgatgaccc ctataaacaa cagaaatgga
1501 ccaacaaatt attaggggac tcatagtgga acagcccgtg atacctgtcc gtaatatcta
1561 ttacatgctt acctatgcat ggggttattt acaggaaatt aagcaggcaa accttgaagc
1621 catacccggt aacaatcttc ttgatatcct ggggtatgta ttaaataaag gggttttaca
1681 gctttcacgc cgagggcttg agcttgatta caatcctaac accgagatca ttcctggcat
1741 caaagggcga atagagtttg ctaaaacaat acgcggcttc catcttaatc atgggaaaac
1801 cgtcagtact tttgatatgc ttaatgaaga cacgctggct aaccgaatta taaaaagcac
1861 attagccata ttaattaagc atgaaaagtt aaattcaact atcagagatg aagctcgttc
1921 actttataga aaattaccgg gcattagcac tcttcattta actccgcagc atttcagcta
1981 tctgaatggc ggaaaaaata cgcgttatta taaattcgtt atcagtgtct gcaaattcat
2041 cgtcaataat tctattccag gtcaaaacaa aggacactac cgtttctatg attttgaaag
2101 aaacgaaaaa gagatgtcat tactttatca aaagtttctt tatgaatttt gccgtcgtga
2161 attaacgtct gcaaacacaa cccgctctta tttaaaatgg gatgcatcga gtatatcgga
2221 tcagtcactt aatttgttac ctcgaatgga aactgacatc accattcgct catcagaaaa
2281 aatacttatc gttgacgcca aatactataa gagcattttt tcacgacgaa tgggaacaga
2341 aaaatttcat tcgcaaaatc tttatcaact gatgaattac ttatggtcgt taaagcctga
2401 aaatggcgaa aacatagggg ggttattaat atatccccac gtagataccg cagtgaaaca
2461 tcgttataaa attaatggct tcgatattgg cttgtgtacc gtcaatttag gtcaggaatg
2521 gccgtgtata catcaagaat tactcgacat tttcgatgaa tatctcaaat aagcggtaag
2581 ttttatagaa aataccgctc ccgacaccac atcgggagcg gctcatcatc aggaggcgat
2641 cacctccacc agcacatccg ttgcccaact ctcttcgctg ttggcgacat tcccttcctt
2701 gcagg
//