GenBank-Updates@genbank.bio.net (02/28/91)
LOCUS RATKAL3 1301 bp ds-DNA ROD 28-FEB-1991
DEFINITION Rat true tissue kallikrein gene, exons 3-5.
ACCESSION D00448
KEYWORDS kallikrein; protease; serine protease.
SEGMENT 3 of 3
SOURCE Rat (adult Sprague-Dawley) kidney mRNA, cDNA clones lambdaRKK1,
RKK7, and liver DNA, genomic clone lambdaRKG24.
ORGANISM Rattus norvegicus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1301)
AUTHORS Inoue,H., Fukui,K. and Miyake,Y.
TITLE Identification and structure of the rat true tissue kallikrein gene
expressed in the kidney
JOURNAL J. Biochem. 105, 834-840 (1989)
STANDARD full staff_entry
COMMENT The 5'-flanking region is compared with those of human and mouse
kallikrein genes, in [J. Biochem. 105, 834-840 (1989)].
FEATURES Location/Qualifiers
intron <1..70
/note="kallikrein intron 2"
CDS join(D00446:807..864,D00447:151..310,71..357,455..591,
952..1107)
/note="kallikrein precursor"
/codon_start=807
mat_peptide join(D00447:177..310,71..357,455..591,952..1104)
/note="renal kallikrein mature peptide"
/codon_start=177
exon 71..357
/note="kallikrein (exon 3) (AA at 72)"
intron 358..454
/note="kallikrein intron 3"
exon 455..591
/note="kallikrein (exon 4)"
variation 545..545
/note="t in lambdaRKK1, lambdaRKG24; c in lambdaRKK7"
intron 592..951
/note="kallikrein intron 4"
exon 952..1107
/note="kallikrein (exon 5)"
misc_signal 1131..1136
/note="polyA signal"
BASE COUNT 301 a 365 c 333 g 302 t
ORIGIN About 0.6 Kb after segment 2.
1 tttccctgta ctctgcccaa tctccctggt tgcctcttct cattcaatcc atctccttcc
61 ctgccctcag caattaccag gtttggctgg gccgaaacaa cctatatgaa gatgaaccct
121 ttgctcagca ccggcttgtc agccaaagct tccctcaccc cggtttcaac caggacctca
181 tatggaacca cacccgacaa cctggggacg actacagcaa tgatttgatg ctgctccacc
241 tcagccagcc tgcggacatc acagatggtg tgaaggtcat cgatctgccc attgaggagc
301 ccaaggtggg gagcacctgc cttgcctcgg gctggggcag catcacacct gacggatgta
361 agtctgggcc aggcaacaaa gctgggtgtg agggggaggc agagaggagg tggctgggtt
421 ctgctcgatg accctcttgc ctcctgatcc acagtggaat taagtgatga tctccagtgt
481 gtgaacatcg atcttctgtc taatgagaag tgcgtcgagg cacacaaaga agaggtgaca
541 gatctcatgc tgtgtgcagg agagatggat gggggcaaag acacttgcaa ggtgagacag
601 cccctccctg cagtgaggtg aagggctgag agagggaact gaggttctcc attcccactt
661 ccacaccctg tctaagcaga tgcttggttt ccttccttgt ggaagtgatg tgagctggga
721 gtggaggatc ccggtggtac tattcctcct cattgacttg gcttcaggga ccggcctgtc
781 cacccctcac tccctgtaag agctggcctc ccaggagctg tgttctctcc cctactggcc
841 agagctgcct cttagagtcc cctttcttcc tcccactgaa gtcagttcca ggttgacaag
901 tagaacccaa ctgccaactc cttcttacac ctgtccctct ccttctttca gggtgactca
961 ggaggccccc tcatctgtaa tggtgtgctc caaggcatca cgtcctgggg ctttaaccca
1021 tgcggtgaac ccaagaagcc aggcatctac accaaactta ttaagttcac cccctggata
1081 aaagaagtta tgaaggaaaa cccctgagtg tcacacagtc ccctggtctc aataaaaccc
1141 accatgcagc acacgtgttc aagttctgat tctctctcgc cttctggaat ctggagctgg
1201 gaaacaccca tctaaggtca ggacttctaa agcagatcgg caagggatag gggtcatgta
1261 tggttggata aagggatggg gttgagacaa aggacgacaa a
//