GenBank-Updates@genbank.bio.net (02/28/91)
LOCUS HUMACTSG2 3351 bp ds-DNA PRI 28-FEB-1991
DEFINITION Human enteric smooth muscle gamma-actin gene, exons 2 and 3.
ACCESSION D00649
KEYWORDS actin; gamma-actin.
SEGMENT 2 of 7
SOURCE Human genomic DNA (cell line Hut-14), clone HA-315.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 1 to 3351)
AUTHORS Miwa,T., Manabe,Y., Kurokawa,K., Kamada,S., Kanda,N., Bruns,G.,
Ueyama,H. and Kakunaga,T.
JOURNAL Unpublished (1990)
STANDARD full staff_entry
REFERENCE 2 (bases 2093 to 2254; 3135 to 3263)
AUTHORS Miwa,T. and Kamada,S.
TITLE The nucleotide sequence of a human smooth muscle (enteric type)
gamma-actin cDNA
JOURNAL Nucleic Acids Res. 18, 4263-4263 (1990)
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Takeshi Miwa
Department of Oncogene Research
Research Institute for Microbial Diseases
Osaka University
3-1 Yamadaoka
Suita
Osaka 565
Japan
Phone: 06-875-2470
Fax: 06-875-1292
FEATURES Location/Qualifiers
precursor_RNA <1..>3351
/note="mRNA and introns"
intron <1..2092
/note="intron 1"
exon 2093..2254
/note="gamma-actin exon 2"
intron 2255..3134
/note="intron 2"
exon 3135..3263
/note="gamma-actin exon 3"
intron 3264..>3351
/note="intron 3"
BASE COUNT 830 a 784 c 775 g 962 t
ORIGIN 4.5 kb downstream of segment 1; chromosome 2.
1 gaattcatat tcatttcatt aaatttaacc ttggctgtgt gtggtgggcc acttgtaatc
61 tcagcacttt gggaggctga ggcaggagaa tcacttgaac ccaggagttc agaccagcct
121 gggcaacaga gggagactct gtttctacag aaaccaattt ttatatcagc cagccataga
181 gcatgcctgt gatcccagct acttgggatg ctgaggtggg aggattgctt gggctgggag
241 gtcgagtctg cagtgactgt gtctgcactg tactgcagct tgggtgacag agcaagaccc
301 catctcaaaa aatatatata tatatatctt ctgattcagc tcattttctg tgccttttga
361 gatcttcttg gacttttatt ctttcaccca gtgtatttaa tattactctc tcccagctgc
421 ttttatctga aaactttatc aacctgtgct ttatattttc attcaattca tttataaaaa
481 tgtcacatga gaaagggtca agagtagagg ctgacatatg ctgctctcat tgacattgac
541 tagctgccgc aaatatacaa ttgtattatt gcttactaca tgctttatca agaggatatc
601 ctattctgga cctgtgggct ggtttagaga gtgaggactc atcactgggg ctatgggaga
661 cataagatca gcagagacag agcaaagctg atcccttgtt gtcacagcag gcctctccct
721 agtatgagcc ttgctttgaa catatctgga aggcttcctt tagtcttgaa aatgtttaca
781 agcctctgct cagcatctac ctagccttca gacctcgcgt tctcaggcct gtggtgctgt
841 ctcctctctg cttcagtatg gtcttctctg tccagcttct gcatgaggcc atctggggcc
901 aagggtcaca ggttttagaa gttcccctct ccccgctcca tgccatagcg acctgcaatc
961 gcacatctgg ataaaacttc agccggcctt ctctttatgt gcctggcgcc tctcttttct
1021 ctgggttttt ggaagtctgc ctgcccagcc cctcagctgg ggccttcccc acttctgccc
1081 cgccccactg gttcctccca gggtaggagg caatctctga ctgtcttccg aggctctgtt
1141 gcttctcctt catcaccaaa tgccaggaat ttgtcagatg ctgtttgtaa ctcaaaagaa
1201 agaaagaaaa agaaaaagat acaggaagga aggaaggcag aaaaagagaa agaaagaatg
1261 cgtgcagcag atgttgggaa agttaatttc ttcattattt tgcatcatcc agttcggatc
1321 tcagcatggg gtagggaatc ctctgttgtc cccatctgtc gaggcaacag tgagtcccat
1381 catggagtcc cttcttttcc tctctcctcc cagagtgccc cttcctcatc aaggtgcttc
1441 tacccttagg agtgtacatt tcacctataa ccactgccta ttttttccta gtgatatgtt
1501 atgctattga tttttaatta tataaactat atgcaaatgc agcctccttg taaaaaaatt
1561 aaaatattat agatataacc cagcacttct tctcccagtt ctttccacac tcccagaggt
1621 aatcatgata atcagtttgc taggtgtctt ccagaacttt tagcttcttt ctttggcttg
1681 tatatggcca ccttctcact gcatcctccc tgtggcctac cctctgcatg catctggatc
1741 ctaattccct cttcttataa agacacccat tgtactggag tagtcatact ggatgacagc
1801 catattgcat taatctcatt ttaacttaat tacctcttta aagaccctat ctccaaatac
1861 agtcaccttc tgtgtactgg gggttaggac ttcaacatga atttttgggg ggacacaatt
1921 cacatttcag ggcagaggga ggaggctcaa ctgccagtga gtgggaggcc aagccatgac
1981 tgtacctact ggaagtgtga actgattgca ggtagggtaa ggaaaccaag aaactgtctc
2041 caaactgatt gaacaagtgg ctgactagtt ctcttctccc ccaaatccca aggtgctcca
2101 gtccccagct cactcagcca catacaccat gtgtgaagag gagaccaccg cgctcgtgtg
2161 tgacaatggc tctggcctgt gcaaggcagg cttcgcagga gatgatgccc cccgggctgt
2221 cttcccctcc attgtgggcc gccctcgcca ccaggtgcgt gctcatctgg ataccaccag
2281 gctttgagcc actaggagta agcgctgcac tgtggagacc ctgctgagct gggggttagg
2341 ggaaggaaat tgtgtgtgtg tgtgtgtgtg tgtctgtgca tgcacatgcg tgtgtgcacc
2401 gcaggtgtag ggagttcttt tctgattaac tgatagagca ggacccttta aatagaaata
2461 tccagggcca aaaccaacat acatcaggca cacaatgaca ctactatgtc tggcttacat
2521 ttggggggtt gccaggtttg gaaaaattaa tttatatggg attgaacaag cctcttgcct
2581 ttaagaaatt tacactttgt gaagaaatac acataactaa ccagagatca tgggctgtaa
2641 tagaggtaaa atatttcaca ggaagggaaa agtatcacag aaaaagtaat atctgagaat
2701 aagaaggatt tcagcaggtg gagagatagc aaagatttgg agatctaagc aggcacaggg
2761 gacttgagga ggatgcatac aagtcgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtctgtg
2821 tgtgtgtgtg tgtgtgtgtc tgtgtgtagt aggatgaggg gaagaagtgc tatggaggct
2881 tgttttatct cagaaaacat cgttgtctga agagaaagaa gaaaggccac cacacaagaa
2941 atcctccacc ttctcttcct gacaccttca tactcactgg catctgctcc atcctgtctc
3001 ctgctatcct gtttctgggg gaagaaaggc tgttttcccc atccttatcc ttcttcagag
3061 accttccagt gcctgaatcc ctctgtgtgg agccctcagc cattcatttc tcttttcttc
3121 tttttgcatt gcagggtgtg atggtgggaa tgggccagaa agacagctat gtgggggatg
3181 aggctcagag caagcgaggg atcctaactc tcaaataccc cattgaacac ggcatcatca
3241 ccaactggga tgacatggag aaggtatctg tagacttccc cttaatgagc ctgctttaat
3301 gatcacccat cgtcatgacc ctgtattcat aggccactgt gctctgtcga c
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