GenBank-Updates@genbank.bio.net (03/01/91)
LOCUS VIBGCAT 1366 bp ds-DNA BCT 01-MAR-1991
DEFINITION Aeromonas hydrophila gene for phospholipid-cholesterol.
acyltransferase.
ACCESSION X07279 M59478
KEYWORDS glycerophospholipid-cholesterol acyltransferase.
SOURCE Aeromonas hydrophila.
ORGANISM Aeromonas hydrophila
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Vibrionaceae.
REFERENCE 1 (bases 1 to 1366)
AUTHORS Thornton,J., Howard,S.P. and Buckley,J.T.
TITLE Molecular cloning of a phospholipid-cholesterol acyltransferase
from Aeromonas hydrophila. Sequence homologies with
lecithin-cholesterol acyltransferase and other lipases
JOURNAL Biochim. Biophys. Acta 959, 153-159 (1988)
STANDARD simple staff_entry
REFERENCE 2 (bases 1 to 1366)
AUTHORS Hilton,S., McCubbin,W.D., Kay,C.M. and Buckley,J.T.
TITLE Purification and spectral study of a microbiol fatty
acyltransferase: Activation by limited proteolysis
JOURNAL Biochemistry 29, 9072-9078 (1990)
STANDARD simple staff_entry
COMMENT *source: clone=pHEC2.2; strain=Ah65.
Biochemistry 29,9072-9078 (1990) presents a corrected coding
sequence nested within the sequence presented in Biochim. Biophys.
Acta 959,153-159 (1988).
FEATURES Location/Qualifiers
CDS 238..1245
/note="precursor protein"
/codon_start=238
sig_peptide 238..291
/note="put. signal peptide (AA -18 to -1)"
/codon_start=238
mat_peptide 292..1242
/note="phospholipid-cholesterol acyltransferase (AA
1-281)"
/codon_start=292
BASE COUNT 294 a 451 c 376 g 245 t
ORIGIN
1 catgctcctg tcattggtac gggagcagtg tgtgcaagcc gggcgtgaaa caaacgtgaa
61 gcctccccat ctgggtgaag ggggtgtgac cgtaatcgat ttaatcagca ccgcctgctg
121 cgaaatttaa ttcggctgga taccctgcaa aatggcaaaa cagcttccca gcacctttgc
181 ctatcgagga aaagccctgc aggcaccacc tgctttcaaa gcaacgagaa caacaagatg
241 aaaaaatggt ttgtgtgttt attgggattg gtcgcgctga cagttcaggc agccgacagc
301 cgtcccgcct tctcccggat cgtgatgttt ggcgacagcc tctccgatac cggcaagatg
361 tacagcaaga tgcgcggtta cctcccctcc agccccccct actatgaggg ccgcttctcc
421 aacgggcccg tctggctgga gcagctgacc aacgagttcc cgggcctgac catagccaac
481 gaggcggaag gcggaccgac cgccgtggct tacaacaaga tctcctggaa tcccaagtat
541 caggtcatca acaacctgga ctacgaggtc acccagttcc tgcaaaaaga cagcttcaag
601 ccggacgatc tggtgatcct ctgggtcggc gccaacgact atctggccta tggctggaac
661 acagagcagg atgccaagcg ggtgcgcgac gccatcagcg atgcggccaa ccgcatggtg
721 ctgaacggcg ccaaggagat actgctgttc aacctgccgg atctgggcca gaacccctcg
781 gcccgcagcc agaaggtggt cgaggcggcc agccatgtct ccgcctacca caaccagctg
841 ctgctgaacc tggcacgcca gctggctccc accggcatgg tgaagctgtt cgagatcgac
901 aagcagtttg ccgagatgct gcgtgatccg cagaacttcg gcctgagcga ccagaggaac
961 gcctgctacg gtggcagcta tgtatggaag ccgtttgcct cccgcagcgc cagcaccgac
1021 agccagctct ccgccttcaa cccgcaggag cgcctcgcca tcgccggcaa cccgctgctg
1081 gcccaggccg tcgccagccc catggctgcc cgcagcgcca gcaccctcaa ctgtgagggc
1141 aagatgttct gggatcaggt ccaccccacc actgtcgtgc acgccgccct gagcgagccc
1201 gccgccacct tcatcgagag ccagtacgag ttcctcgccc actgatgaca agccgacact
1261 cggtcggccc ctcttttgcc tcgtcacgac accatcaatc agcgatagcg cttcactatg
1321 ccgtcataga tacgttctgc cggatggtgg ccagcgcctc ctgcag
//