GenBank-Updates@genbank.bio.net (03/01/91)
LOCUS BSUTERC 3196 bp ds-DNA BCT 01-MAR-1991
DEFINITION B.subtilis replication termination protein (terC) gene, 3' end.
ACCESSION X06168 M36987
KEYWORDS replication termination protein.
SOURCE B.subtilis (strain GS168) DNA, clone pWS10.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 3196)
AUTHORS Carrigan,C.M., Haarsma,J.A., Smith,M.T. and Wake,R.G.
TITLE Sequence features of the replication terminus of the Bacillus
subtilis chromosome
JOURNAL Nucleic Acids Res. 15, 8501-8509 (1987)
STANDARD simple staff_entry
REFERENCE 2 (sites)
AUTHORS Ahn,K.-S. and Wake,R.G.
TITLE A unique open reading frame adjacent to the replication terminus of
the Bacillus subtilis W23 chromosome compared with Bacillus
subtilis 168
JOURNAL Unpublished (1990)
STANDARD full staff_review
COMMENT The site of arrest of the clockwise fork, which defines terC, has
been localized near inverted repeat B/B'
Data for [Nucleic Acids Res. 15, 8501-8509 (1987)] kindly reviewed
(15-SEP-1988) by WAKE R.G.
Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by R.G.Wake, 24-JUL-1990
Author address: R.G.Wake
University of Sydney, Dept. of Biochemistry
NSW 2006, AUSTRALIA
E-mail:GERRY@EXTRO.UCC.SU.OZ.AU
FEATURES Location/Qualifiers
CDS complement(<1..358)
/note="ORF 119+"
/codon_start=358
misc_feature 1..1267
/note="terC locus"
CDS complement(429..734)
/note="ORF 101"
/codon_start=734
misc_signal 1607..1630
/note="terminator (pot.)"
misc_binding 1887..1892
/note="ribosome binding site (pot.)"
CDS 1901..2269
/note="replication terminator protein (rtp; terC)"
/codon_start=1901
misc_signal 2277..2310
/note="terminator (pot.)"
CDS 2417..3190
/note="ORF 257"
/codon_start=2417
BASE COUNT 1006 a 617 c 692 g 881 t
ORIGIN 5 bp upstream of PstI site.
1 aagcttgcgg cagcccgcat aaatccagca tggtaataag gttatgtctc atcgatccgg
61 agaagcccgc tgctattttt gtgtctactg ccatttgctc aagagtcccg ccttgcagca
121 ggctttcggc ggctgtttcg tacgctgtct tcatctggga aaaaccaggc gtgatcccgg
181 caatcacaag cgaggcatcc cgattgataa attcattatg aggtgaatag tagatgccaa
241 gttcgttttc ttgatggaga cgaaatggat cagtaagtaa atcggacttt gtaagtgaag
301 agggaagaaa atgtactttt tctgcgaact cttttaagca gctgcggttt ataatcatgt
361 gtaagcctcc tcgaaaaatg agatcccatt tgtccattct gatcgtaaca acgggatctc
421 attcatcctt atctgctatt aaaatgtcct gcctgccaca cctctttgac tgcttgggga
481 acttctttca cttcagctgc ggtctgctga agcgtgtatt tttttatttg gacatcctgc
541 tgaatcttct tttgtttttg cgtcagcagc tctgtttctg tttttaatcc ttcgatttgt
601 ttttgcatag cctgcgtaac agaggaaagc tcctttagtg cggggtctat ttttttcttg
661 ttttggatca cggtaactcc taagaagata atagaaactg ctaatactgc aagactgata
721 taaactataa tcatatgttc actccttttc ttatgtcatt accctgtatc cgttttttta
781 atcagaagaa atgatattta cacagttcgt ttgatttgcg tattggatgt attgtactta
841 taatgtgata tcgtacatat gtaaacaaag aaagggtgct tcaattgcaa agtttacaac
901 ataaaactgc actaatcaca ggcggaggca gaggaattgg ccgcgctact gctctggcgc
961 ttgccaaaga aggcgtgaat atcgggttaa ttggccggac ttccgctaat gtagaaaaag
1021 tggctgaaga agtcaaagcg cttggtgtaa aagctgcatt tgctgctgca gatgtaaaag
1081 atgccgatca ggttaaccaa gctgtagctc aagtgaagga acagctcggt gatatcgata
1141 tcctcattaa taatgccggc atcagcaaat ttggcggttt cttagatctg tcagctgatg
1201 agtgggaaaa tattattcaa gtcaacctaa tgggtgtgta ccatgtcact cgcgcggtgc
1261 ttccggaaat gatcgaacgc aaagccggag acatcattaa tatttcatct acagcgggcc
1321 aaagaggagc tgctgtaaca agtgcttaca gcgcttctaa atttgccgtt ctcgggttaa
1381 cagagtctct tatgcaagaa gtgagaaaac ataatatcag agtcagcgcg ttaacgccga
1441 gcactgtcgc tagtgatatg tctatcgaat tgaacttaac agacggtaat cctgaaaaag
1501 ttatgcagcc tgaggatctt gctgagtaca tggtggcaca actgaaatta gatcctcgta
1561 ttttcatcaa aacagcggga ttatggtcaa caaatcctta aaaatgaaaa cctgtctttc
1621 gacaggtttt tttatttgaa tgaaatccgt accggtaaaa tgagatatgt aaaccctggc
1681 aatcgtttaa attgaagata gcagtaaatg caggctataa tagaactaag aaaactatgt
1741 accaaatgtt cagtcgaaat ttattttttc cgctacacct ataatcagta aacatgaaat
1801 aactggacta tcagtcttta atataaagaa ggaaaacaat aaaagaaaat tgaatattta
1861 gtacatagtg ttgtcagtga cagaagaagg agtgcatatg atgaaagaag aaaaaaggag
1921 ttcaacaggc tttttagtga aacagcgcgc atttttgaag ctttatatga taacgatgac
1981 agagcaagag agactctatg ggttaaagct gcttgaagta cttcggtctg aatttaaaga
2041 gattggtttt aaaccaaatc atacagaagt ataccggtct ttgcatgagc ttcttgatga
2101 cgggatacta aaacaaatta aagtaaaaaa agaaggggct aagctccagg aagtcgtcct
2161 ctatcaattt aaagattacg aagctgccaa gctatataaa aaacagctga aggtagagct
2221 ggatcgctgt aaaaaactga ttgaaaaagc tctctcagat aatttttaat agaaacaaac
2281 acccggcgca cgagtgtgaa ccgggtgttt tctgcttttc attatacata tttgtcgaaa
2341 agaaacaata caaatcaata atggccttca aacttgacat catttcctca cgtggtaaca
2401 ttttaaacgt gtgaaaatgc gaacaaaaaa gcgaacaaag gagatgttac cgatctttga
2461 tcaaaagaaa gtagccttta taggagcagg atctatggcg gaaggaatga tatcaggtat
2521 cgttcgagcc aacaaaatcc cgaaacaaaa catctgcgtt acaaaccgca gcaatacaga
2581 gcgattaact gaacttgaac ttcaatatgg cattaaaggc gccttgccga accaactatg
2641 tattgaagac atggatgtgc tcattttggc aatgaagcca aaagacgccg aaaacgctct
2701 gtcatcacta aaatcacgta ttcaacccca tcaattaata ttgtcggtcc ttgccggcat
2761 aactacctct tttattgaac aatcattgct taatgaacag ccggttgtca gagtgatgcc
2821 caacacttcc agtatgattg gcgcttctgc aacagctatt gcccttggca aatatgtgtc
2881 ggaagacctg aagaagctgg ccgaagcatt gctcggatgc atgggcgaag tctatacaat
2941 ccaagaaaat caaatggata tattcaccgg aattgcaggc agcggacccg cgtattttta
3001 ttatttaatg gaattcattg aaaagacagg cgaggaagcg ggcctcgata aacagttatc
3061 tcgaagcatc ggcgcacaga cacttttagg tgccgcaaaa atgctcatgg aaacaggaga
3121 acatcctgag atcttaagag ataatatcac gtcaccgaac ggaacgacag cagcggggct
3181 cgaagcttga aaaaga
//