GenBank-Updates@genbank.bio.net (03/01/91)
LOCUS MYRCYNHY 1235 bp ds-DNA PLN 01-MAR-1991
DEFINITION M.verrucaria cyanamide hydratase (cah) gene, complete cds.
ACCESSION M59078
KEYWORDS cyanamide hydratase.
SOURCE M.verrucaria DNA.
ORGANISM Myrothecium verrucaria
Eukaryota; Plantae; Thallobionta; Basidiomycotina;
Deuteromycotina.
REFERENCE 1 (bases 1 to 1235)
AUTHORS Maier-Greiner,U.M., Obermaier-Skrobranek,B.M., Estermaier,L.M.,
Kammerloher,W., Freund,C., Wuelfing,C., Burkert,U.I., Matern,D.H.,
Breuer,M., Eulitz,M., Kuevrevioglu,O.I. and Hartmann,G.R.
TITLE Isolation and properties of a nitrile hydratase from the soil
fungus Myrothecium verrucaria highly specific for the fertilizer
cyanamide and cloning of its gene
JOURNAL Unpublished (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS join(281..500,564..1078)
/EC_number="4.2.1.69"
/product="cyanamide hydratase"
/gene="cah"
/codon_start=281
exon <281..500
/number=1
/gene="cah"
/evidence=EXPERIMENTAL
exon 564..>1078
/number=2
/gene="cah"
/evidence=EXPERIMENTAL
BASE COUNT 323 a 287 c 294 g 331 t
ORIGIN
1 cggctcttat acgacattga ttagaaatgc aattgacgca aaatggaagt tatacgacaa
61 gcgcaataat ggattgagca cttatcgaac acgtacgtca tagtgaacat tggccactgt
121 tgtctctggt cccctcaaac gtataaccct tattgaggac ttgggtatat atacacgcac
181 tatccgtcat gtttcacgaa aggtcgacct catactccta cgtttctcaa actagtacga
241 tcctacttcc tcgcttatct gctctaaacg attcaacaag atgtcttctt cagaagtcaa
301 agccaacgga tggactgccg ttccagtcag cgcaaaggcc attgttgact ccctgggaaa
361 gcttggtgat gtctcctcat attctgtgga agatatcgcg ttccctgcgg cagacaaact
421 tgttgccgag gcacaggcct ttgtgaaggc ccgattgagt cccgaaacct acaatcactc
481 catgcgcgtt ttctactggg gtaagtcatg ccgtgctgcg tgcctgacat aattccaaat
541 tctcacaaat attttccaac aaggaaccgt catcgcgaga cgtttacttc ccgagcaagc
601 taaagacttg tctccaagta catgggcact gacatgtctt ctgcatgacg ttggtactgc
661 ggaggcatac tttacatcta cacgaatgtc cttcgatatt tacggtggca ttaaggctat
721 ggaggtgctc aaggtccttg ggagtagcac cgaccaggct gaggctgttg ccgaggccat
781 cattcgtcat gaggatgtgg gggtagatgg caacatcaca ttcctcggtc agttgatcca
841 gctggctacg ctttatgaca atgtcggggc ctacgatggg attgatgatt ttggtagctg
901 ggttgatgac accacacgca acagtatcaa cacggcattc ccacgacatg gttggtgttc
961 ttggtttgcc tgcacggttc gtaaggaaga aagtaacaag ccttggtgcc acacaacgca
1021 tatccctcag ttcgataaac agatggaagc gaacactttg atgaagcctt gggagtaact
1081 ctgagtaagc agagaatatt tagccgggta gctatagatg aatctggaca aattcaggca
1141 catttggttt cacgatacag gtattggaaa tagcttgcag gaaggtatca tgtcaacacc
1201 atgtatttta ttttccctcg gtgatttaaa tgggg
//