GenBank-Updates@genbank.bio.net (03/01/91)
LOCUS STRGTFIA 5100 bp ds-DNA BCT 01-MAR-1991
DEFINITION S.sobrinus glucosyltransferase (gtf-I) gene.
ACCESSION D90213
KEYWORDS glucosyltransferase; gtf-I gene; mutasynthetase; water-insoluble
glucan.
SOURCE S.sobrinus (strain 6715) genomic DNA.
ORGANISM Streptococcus sobrinus
Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
Streptococcaceae.
REFERENCE 1 (bases 1 to 5100)
AUTHORS Abo,H., Matsumura,T., Kodama,T., Ohta,H., Fukui,K., Kato,K. and
Kagawa,H.
TITLE Peptide sequences for sucrose splitting and glucan binding within
Streptococcus sobrinus glucosyltransferase (water-insoluble glucan
synthetase)
JOURNAL J. Bacteriol. 173, 989-996 (1991)
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Hirokazu Abo
Department of Microbiology
Okayama University Dental School
2-5-1 Shikata-cho
Okayama 700
Japan
Phone: 0862-23-7151 x5251
Fax: 0862-22-4572
FEATURES Location/Qualifiers
misc_signal 109..114
/note="-35 signal"
misc_signal 132..137
/note="-10 signal"
misc_signal 166..172
/note="SD sequence"
CDS 181..4959
/note="glucosyltransferase precursor"
/codon_start=181
sig_peptide 181..294
/note="glucosyltransferase signal peptide"
/codon_start=181
mat_peptide 295..4956
/note="glucosyltransferase mature peptide"
/codon_start=295
misc_signal 4990..5016
/note="put. transcriptional termination sequence"
misc_signal 5042..5059
/note="put. transcriptional termination sequence"
BASE COUNT 1575 a 1051 c 1111 g 1363 t
ORIGIN
1 taagatcagt cctcaacaga aattgcctta tttaaaaaga aattaagaaa aaaacgttta
61 atataatgtt aataactaga aataaattgc aaattgtggt aaaattaatt gatggttaga
121 ataatttaac tcgtaaaaaa gttaactgtc gttatttttc aaattaggag gactccattc
181 atggagaaga atgtacgttt taagatgcat aaggtgaaaa agagatgggt aaccctctct
241 gtcgcatctg ccaccatgtt ggcatcagcc cttggtgctt cagtagctag tgcggataca
301 gacactgcta gtgatgatag caaccaaacc gtggtaactg gtgaccagac tactaacaat
361 caagccactg accagacttc tattgcagca acagctacat cagaacagtc tgcttcaact
421 gatgcagcaa cagatcaagc atcagcagca gagcaaactc aaggaacaac agctagcaca
481 gacacggcag ctcaaacaac cacaaatgct aatgaagcta agtgggttcc gactgaaaat
541 gagaaccaag gttttacaga tgagatgtta gcagaagcca agaatgtggc tactgctgaa
601 tctgattcaa ttccatcaga cttggctaaa atgtcaaatg ttaagcaggt tgacggtaaa
661 tattattact acgaccaaga tggcaatgtt aagaaaaact ttgctgtcag cgttggtgat
721 aagatttatt actttgatga aactggcgct tacaaggaca ctagcaaggt tgatgccgac
781 aagtccagtt cagctgtaag tcaaaatgca acaatatttg cagctaataa ccgtgcctac
841 agcacctcag ctaaaaattt tgaagccgtt gataactacc tgacagctga ctcttggtat
901 cgtccaaaat caatcctgaa agacggaaaa acttggacag aatctggcaa agatgacttc
961 cgcccgcttc tcatggcttg gtggcctgat accgaaacca aacgtaacta cgttaattac
1021 atgaacaagg ttgttggtat tgataagacc tataccgctg aaaccagcca agctgattta
1081 acggcagcag cagaattggt tcaagctcgt attgaacaaa aaattacaag tgaaaataac
1141 actaagtggc tccgtgaggc gatttctgcc tttgtgaaaa ctcagccgca atggaatggt
1201 gaaagcgaaa agccttacga tgatcacttg caaaatggtg ctcttctctt tgacaatcaa
1261 actgatttaa caccagatac gcaatcgaac tatcgtttgc tcaatcgcac accaacaaac
1321 caaactggtt ccttggattc tcgtttcacc tataacccaa atgacccact gggcggctat
1381 gatttccttt tagccaacga tgttgataat tccaatccag tcgtgcaagc ggaacaactc
1441 aactggctgc actacctgct caactttggc tctatctatg ccaatgatgc agatgccaat
1501 tttgactcaa tccgtgtaga tgcggaagat aatgttgatg ctgaccaact gcaaatctct
1561 agtgattacc ttaaggcagc ttacggtatc gataaaaaca acaaaaatgc aaataaccac
1621 gtttctatcg tagaagcatg gagcgacaac gatacccctt atctccatga tgatggcgac
1681 aacctcatga acatggacaa caagttccgt ttgtccatgc tttggtcttt agctaagcca
1741 accgatgttc gttctggttt gaatcctttg atccacaaca gtctggttga ccgtgaagtg
1801 gatgaccgtg aagttgaaac cgttccaagt tacagctttg ctcgggctca tgatagtgaa
1861 gttcaggata tcattcgtga tattattaag gctgagatta atccaaattc atttggttat
1921 tcattcaccc aagaagaaat tgatcaagct ttcaagattt acaacgaaga tctcaagaag
1981 tctgataaaa aatacactca ctacaatgtg ccgctttctt ataccttgct tctgactaac
2041 aagggttcga ttcctcgcgt ctattatgga gatatgttca ccgatgatgg tcaatacatg
2101 gccaacaaga ctgtgaacta cgatgctatc gaatctctgc tgaaagcccg tatgaagtac
2161 gttgctggtg gtcaggctat gcagaattac caaatcggta atggcgaaat cttgacttct
2221 gtccgttatg gtaagggtgc ccttaaacaa agcgataagg gtgatgcgac aactcgtacg
2281 tcaggtgtcg gcgttgttat gggaaaccaa cccaacttta gcttggatgg aaaggttgta
2341 gccctcaaca tgggtgctgc ccacgctaac caagaatacc gtgctcttat ggtatcaact
2401 aaagacggtg ttgcaaccta tgctacagat gctgatgcta gcaaggctgg tctggttaag
2461 cgcacagatg aaaatggtta cctctacttc ttgaacgacg atctcaaggg ggttgctaac
2521 cctcaggttt ctggtttcct tcaagtctgg gtaccagtgg gagcagcaga tgaccaagat
2581 attcgtgtag cagctagcga tacagcaagt accgatggaa aatcactcca tcaagatgct
2641 gccatggact ctcgcgtcat gtttgaaggt ttctctaact tccaatcttt tgcgacaaaa
2701 gaagaagagt ataccaatgt tgtcattgct aacaatgttg ataaatttgt ttcatgggga
2761 atcactgact ttgaaatggc tcctcagtat gtctcatcta ctgacggtca gttccaagat
2821 tctgtcattc aaaatggtta tgcctttacc gaccgttatg acttgggtat gtctaaagca
2881 aacaagtatg gtacagccga ccaattggtt aaggctatca aggctctcca tgctaagggc
2941 ctgaaggtta tggcagactg ggttccagac caaatgtaca ccttccctaa acaagaagtg
3001 gtcactgtta ctcggacaga taagtttggc aaaccaatcg caggaagcca aattaatcac
3061 agtctctacg taacagatac aaagagctct ggtgatgact atcaagctaa atacggcggt
3121 gccttccttg acgaattaaa ggaaaaatat ccagaactct tcaccaagaa gcaaatgtct
3181 actggtcagg cgattgatcc atctgttaag attaaacaat ggtctgctaa gtactttaat
3241 ggaagtaata ttcttggccg gggtgccgat tatgtcctca gcgaccaagt cagcaacaag
3301 tacttcaacg ttgccagcga tacactcttc ttaccaagca gcttactcgg caaggtcgta
3361 gagtctggta ttcgttatga tggtaagggt tatatttata actcaagtgc aactggtgac
3421 caagtcaaag caagcttcat taccgaagca ggcaatctat actacttcgg taaagacggt
3481 tatatggtga ctggcgctca aaccattaat ggtgctaact atttcttcct tgaaaatggt
3541 acggctcttc gcaacactat ttatacagat gctcaaggca atagccatta ctacgcaaat
3601 gacggtaaac gctatgaaaa tgaaaatggt taccaacaat ttggtaatga ctggcgttac
3661 ttcaaggacg gtaacatggc tgttggcttg acaactgttg atggcaatgt tcaatacttt
3721 gataaagatg gtgttcaagc taaggataag attattgtca cccgtgatgg taaggttcgt
3781 tactttgacc aacataatgg aaatgctgta accaatacct tcatcgctga caagactggt
3841 cactggtact atctaggtaa agatggtgtc gctgttaccg gtgctcaaac cgttgggaaa
3901 caaaaacttt actttgaagc aaacggtcaa caagttaagg gtgacttcgt aacttctgac
3961 gaaggtaaac tttacttcta cgatgtcgat tcaggtgaca tgtggactga taccttcatt
4021 gaagataagg caggcaattg gttctacctt ggtaaagatg gtgcagctgt gactggtgct
4081 caaactattc gtggccaaaa actttacttc aaggctaacg gccaacaagt caagggagat
4141 atcgtcaagg gtactgatgg taagatccgt tactacgacg ctaaatctgg tgaacaagtc
4201 ttcaacaaga ctgttaaggc cgctgatggc aagacctatg ttatcggaaa tgatggtgtt
4261 gcagttgatc caagcgttgt caaaggacaa accttcaagg atgcttcagg tgctcttcgt
4321 ttctataacc tcaaaggaca actggtaaca ggcagcggtt ggtatgaaac tgcaaatcac
4381 gattgggttt atatccaatc tggtaaagcc ttgactgggg aacagaccat caatggtcaa
4441 catctttact tcaagaaaga tggacatcaa gtcaaaggac aactggtaac aggaactgat
4501 ggtaaggttc gctattatga tgcaaattca ggcgaccaag ccttcaacaa gtctgtaaca
4561 gttaacggta agacttacta cttcggtaat gatggcactg ctcaaacagc gggaaaccct
4621 aagggacaaa ccttcaaaga tggttcagat atccgctttt acagcatgga aggccaatta
4681 gtgactggca gtggttggta ctcaaacgca caaggtcagt ggctttatgt caaaaatggt
4741 aaagtcttga caggcctgca aacagttggt agccaacgtg tttactttga cgaaaatggt
4801 attcaagcta aaggtaaagc agtaaggact tccgacggta agatacgcta cttcgatgaa
4861 aattcaggta gcatgattac caaccaatgg aaagaggtta acggtcgata ttattacttc
4921 ggtaatgatg gcgcacgtat ctaccgtggc tggaactaat atttgagatt ttattttctc
4981 aatacagaga agacttcctc ggactgagga agtctttttt ggtttagcgc tttaagtcca
5041 tttttcttag attagaaaag accacccgtg aaattttccc gagtggtttt gtctatttga
//