GenBank-Updates@genbank.bio.net (03/05/91)
LOCUS ABCALDH 2683 bp ds-DNA BCT 05-MAR-1991
DEFINITION A.polyoxogenes membrane-bound aldehyde dehydrogenase gene, complete
cds.
ACCESSION D00521
KEYWORDS aldehyde dehydrogenase; ethanol oxidation; membrane-bound enzyme.
SOURCE Acetobacter polyoxogenes DNA, clone pAL25.
ORGANISM Acetobacter polyoxogenes
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Aerobic rods and
cocci; Azotobacteraceae.
REFERENCE 1 (bases 1 to 2683)
AUTHORS Tamaki,T., Horinouchi,S., Fukaya,M., Okumura,H., Kawamura,Y. and
Beppu,T.
TITLE Nucleotide sequence of the membrane-bound aldehyde dehydrogenase
gene from Acetobacter polyoxogenes
JOURNAL J. Biochem. 106, 541-544 (1989)
STANDARD full staff_entry
COMMENT The N-terminal amino acid sequence of the mature ALDH was
determined by an automated Edman degradation procedure.
FEATURES Location/Qualifiers
misc_binding 223..227
/note="ribosome binding site (SD)"
CDS 236..2557
/note="aldehyde dehydrogenase precursor"
sig_peptide 236..367
/note="aldehyde dehydrogenase signal peptide"
mat_peptide 368..2554
/note="aldehyde dehydrogenase"
repeat_region 2578..2592
/note="inverted repeat (put. transcriptional terminator)"
repeat_region 2596..2610
/note="inverted repeat (put. transcriptional terminator)"
BASE COUNT 522 a 803 c 873 g 485 t
ORIGIN 1 bp upstream of AvaI site.
1 cccgagggta accaccctgt ccagcaggcc tggcgtgaaa tccaggtgcc gcagtgcggc
61 tactgccagt ccgggcagat catgcaggcc gtgagcctgc tgaaggatta cccctctccc
121 acggatgagg aaatcgatgc cgtcatggct ggcagcctgt gccggtgcat gacctatatt
181 cgcattcgtg aggccatcaa gaaggccgcg gccaccgcac acggagatgc atcaaatggg
241 tagattaaac cgcttccgcc ttggcaagga cgggcgccgt gaacaggctt ccctgtcacg
301 tcgcggcttc cttgtcacct cccttggcgc cggcgtcatg tttggcttcg cgcgtccttc
361 atccgccaac cagattttcc cgctcgaccg gtcgctgccg ggtgacgggg cgttcgaacc
421 caccatctgg tgttcgatcg cacccgatgg ggaaatcacg gtcaacatca tccgcgcgga
481 aatgggccag catatcggca ccgcccttgc ccgcatcatc gcggatgaaa tggaagccga
541 ctggagcaag gtccgcatca actacgtgga caccgacccc aaatgggggc tgatggttac
601 cggtggcagc tggtcggtgt ggatgacatg ggacgtcttc cgccaggctg gcgccgcgac
661 gcgcacggcc atggtcgagg aaggcgcccg cctgctgggc accacgccgg acaagtgcac
721 ggtcgccagc agcatcgtca gtgccggtgg caagcagatc agctttggcg acatcgtggc
781 caaggggcat ccgtcccatg ccttcacgcc cgaggaaatg gccaagctgc cgctcaagcc
841 cgccagcgaa cgcaggctga tcggcaatgc cgaactcaag gcgctggaca ttccggccaa
901 gaccaacggc acggccatct atggcatcga cgccaaggtg gaaggcatgc tgtacggccg
961 ccccaagatg ccgccgacgc gctatggctc caaggtccgt tcggttgacg ataccgaggc
1021 caagaagatc aagggctatg tccgctacct gctgatcgat gatccgtcgc aggtcgtgca
1081 gggctgggtc gtggtgctgg cggaaagcta cagcgccgcc atccgcgcga ccgatgcgct
1141 gaaggttgaa tggacgccgg gtgagacgat ccacacgtcc gaacgcgaca ttcaggaccg
1201 tggccgcgaa ctgatcaaca acaaggcagg cggcgtctac atcttcaatg atgatggcgt
1261 ggaccaggcc tttggcagtg cgcatacggt catggatcag gaatatacct gtgcatccgt
1321 gctgcattac cagctggaac cgaccaacgc gctggccttt gaaaaggacg gtgtttacga
1381 aatccacgcg ggtaaccagt ggcagagcct gatcctgccc acgctggcca agtcgctgca
1441 ggtgcctgaa agcaaggtca tcctgcgcag ctacctgctg ggtggcgggt ttggccgccg
1501 gctgaacggg gattacatga tcccggccgc ccttgcgtcc aaggcgctgg gcggcaagcc
1561 ggtcaagctg atcctgacgc ggtcggatga catgcagttc gattccttcc gctcgccttc
1621 ggtccagcgt gtccgcatgg cgttcgacgc cagcgacagg atcaccgcca tggattacca
1681 ggccgctgcg ggctggccca cgggcgtgat ggccgaagcg tttatggaaa agggcgtgga
1741 tggcaagccg tatgaccagt tcgccatcgc tggtggcgac cactggtacg aagtcggtgc
1801 cttccgggtg cgtgcgctgc gtaatgacct ggcggaaaag acattccgtc ccggctggct
1861 gcgttcggtc agccccggct ggaccagctg gggggtcgag tgcttccttg atgaagtcgc
1921 gcaccgccag aagaaggatc ctgcgcagtt ccgtcttgaa ctgttgaccg ggcagggccg
1981 taacaagggg caggcgcccg attccgtcgg tggcgcgctg cgtcaggccg ctgtcgtgcg
2041 caggcttatg gaaaaggtga actggggcaa gaccagcctg cccaaggaca ccgcgatggg
2101 ccttgccacc acggcggggc aggaacgcgg catgccgacg tgggatcggt gtgtggcgca
2161 ggtgcatgtg gaccgcagca cgggcgtcgt gacatgccag aagctgacca tcctggtcga
2221 tgcgggtacc gtggttgacc ccgatggcgc gaaggcccag accgagggtg ctgcgctgtg
2281 gggcctgagc atggtcctgt tcgagaacac ggaaatcgtc aacggcatgc cggttgaccg
2341 taacctgaac acctatacgc cactgcgtat tgccgacacg ccggaaatgg acatcgagtt
2401 cctgcccagc accgaaaagc cgatgggtct gggtgaaccg ggcacgacgg tggtcggtcc
2461 tgcaatcggc aacgccatat tcaatgccgt gggtgtccgc ctgcgtcata tgccggtccg
2521 tccggcggat gtgctgcgcg gcctgcagaa cggctaggcg catacaccct gaatacgaaa
2581 accccggcat gcggtgcatg ccggggtttt tttattgtct gggataaaaa taaaagtttt
2641 tgggtgccgc cttttttgag gaaggcggca ttacgggaag ctt
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