GenBank-Updates@genbank.bio.net (03/05/91)
LOCUS ASOTAA 2337 bp ds-DNA PLN 05-MAR-1991
DEFINITION Aspergillus oryzae Taka-amylase A [EC 3.2.1.1, alpha-1,
4-glucan-4-glucanohydrolase] gene, complete cds.
ACCESSION D00434
KEYWORDS Taka-amylase A; alpha-1,4-glucan-4-glucanohydrolase.
SOURCE Aspergillus oryzae (RIB40) genomic DNA, clone phi-AA107.
ORGANISM Aspergillus oryzae
Eukaryota; Plantae; Thallobionta; Eumycota; Ascomycotina;
Plectomycetes; Eurotiales; Trichocomaceae.
REFERENCE 1 (bases 1 to 2337)
AUTHORS Tada,S., Iimura,Y., Gomi,K., Takahashi,K., Hara,S. and Yoshizawa,K.
TITLE Cloning and nucleotide sequence of the genomic Taka-amylase A gene
of Aspergillus oryzae
JOURNAL Agric. Biol. Chem. 53, 593-599 (1989)
STANDARD full staff_entry
COMMENT Compared with the amino acid sequence of mature TAA reported by
Toda et al., Trp-385 is inserted and Asp-476 is deleted.
FEATURES Location/Qualifiers
CDS join(118..285,341..379,465..580,658..766,835..1063,
1122..1284,1350..1496,1562..1802,1882..2169)
/note="Taka-amylase A precursor"
mat_peptide join(181..285,341..379,465..580,658..766,835..1063,
1122..1284,1350..1496,1562..1802,1882..2166)
/note="Taka-amylase A, part of"
misc_signal 18..24
/note="putative TATA box"
exon 118..285
/note="Taka-amylase A precursor, exon 1"
sig_peptide 121..180
/note="Taka-amylase A signal peptide"
mat_peptide 181..285
/note="Taka-amylase A, part of exon 1"
intron 286..340
/note="Taka-amylase A intron 1"
misc_signal 326..331
/note="consensus sequence PuCTPuAC of fungal IVS"
exon 341..379
/note="Taka-amylase A precursor, exon 2"
mat_peptide 341..379
/note="Taka-amylase A, exon 2"
intron 380..464
/note="Taka-amylase A intron 2"
misc_signal 441..447
/note="consensus sequence PuCTPuAC of fungal IVS"
exon 465..580
/note="Taka-amylase A precursor, exon 3"
mat_peptide 465..580
/note="Taka-amylase A, exon 3"
intron 581..657
/note="Taka-amylase A intron 3"
misc_signal 639..644
/note="consensus sequence PuCTPuAC of fungal IVS"
exon 658..766
/note="Taka-amylase A precursor, exon 4"
mat_peptide 658..766
/note="Taka-amylase A, exon 4"
intron 767..834
/note="Taka-amylase A intron 4"
misc_signal 814..819
/note="consensus sequence PuCTPuAC of fungal IVS"
exon 835..1063
/note="Taka-amylase A precursor, exon 5"
mat_peptide 835..1063
/note="Taka-amylase A, exon 5"
intron 1064..1121
/note="Taka-amylase A intron 5"
misc_signal 1095..1101
/note="consensus sequence PuCTPuAC of fungal IVS"
misc_signal 1108..1113
/note="consensus sequence PuCTPuAC of fungal IVS"
exon 1122..1284
/note="Taka-amylase A precursor, exon 6"
mat_peptide 1122..1284
/note="Taka-amylase A, exon 6"
intron 1285..1349
/note="Taka-amylase A intron 6"
misc_signal 1331..1337
/note="consensus sequence PuCTPuAC of fungal IVS"
exon 1350..1496
/note="Taka-amylase A precursor, exon 7"
mat_peptide 1350..1496
/note="Taka-amylase A, exon 7"
intron 1497..1561
/note="Taka-amylase A intron 7"
misc_signal 1545..1551
/note="consensus sequence PuCTPuAC of fungal IVS"
exon 1562..1802
/note="Taka-amylase A precursor, exon 8"
mat_peptide 1562..1802
/note="Taka-amylase A, exon 8"
intron 1803..1881
/note="Taka-amylase A intron 8"
misc_signal 1862..1867
/note="consensus sequence PuCTPuAC of fungal IVS"
misc_signal 1869..1874
/note="consensus sequence PuCTPuAC of fungal IVS"
exon 1882..2169
/note="Taka-amylase A precursor, exon 9"
mat_peptide 1882..2166
/note="Taka-amylase A, exon 9"
BASE COUNT 630 a 598 c 535 g 574 t
ORIGIN 252 bp upstream of SalI site.
1 tgcgatgtat cacaacatat aaatactagc aagggatgcc atgcttggag gatagcaacc
61 gacaacatca catcaagctc tcccttctct gaacaataaa ccccacagaa ggcatttatg
121 atggtcgcgt ggtggtctct atttctgtac ggccttcagg tcgcggcacc tgctttggct
181 gcaacgcctg cggactggcg atcgcaatcc atttatttcc ttctcacgga tcgatttgca
241 aggacggatg ggtcgacgac tgcgacttgt aatactgcgg atcaggtgtg ttgttaccta
301 ctagctttca gaaagaggaa tgtaaactga cttgatatag aaatactgtg gtggaacatg
361 gcagggcatc atcgacaagg taaattgccc ctttatcaaa aaaaaagaag gaaaagcaga
421 agaaaaataa aataaaaaga actctagtcc taaccatcac atagttggac tatatccagg
481 gaatgggctt cacagccatc tggatcaccc ccgttacagc ccagctgccc cagaccaccg
541 catatggaga tgcctaccat ggctactggc agcaggatat gtaacgatcg tatttccttt
601 ggaaatatct acctgtcatc tatttctaca tcatatgaac taacttgatg gttttagata
661 ctctctgaac gaaaactacg gcactgcaga tgacttgaag gcgctctctt cggcccttca
721 tgagaggggg atgtatctta tggtcgatgt ggttgctaac catatggttc gtggtccttt
781 gcaactgact tcgcggatat ggttcatttc agtactgaca atgagtaata tcagggctat
841 gatggagcgg gtagctcagt cgattacagt gtgtttaaac cgttcagttc ccaagactac
901 ttccacccgt tctgtttcat tcaaaactat gaagatcaga ctcaggttga gtattgttgg
961 ctaggagata acactgtctc cttgcttgat ctcgatacca ccaaggatgt ggtcaagaat
1021 gaatggtacg actgggtggg atcattggta tcgaactact ccagtaagat atttctccct
1081 cattctacaa cttggctgat cgatgatact tacgaaatca gttgacggcc tccgtatcga
1141 cacagtaaaa cacgtccaga aggacttctg gcccgggtac aacaaagccg caggcgtgta
1201 ctgtatcggc gaggtgctcg acgttgatcc ggcctacact tgtccctacc agaacgtcat
1261 ggacggcgta ctgaactatc ccatgtatgg ttcctccaac catgagcctt cttgcaagtc
1321 tcatctccta acgaaacggc taaaaccagt tactatccac tcctcaacgc cttcaagtca
1381 acctccggca gcatggacga cctctacaac atgatcaaca ccgtcaaatc cgattgtcca
1441 gactcaacac tcctgggcac attcgtcgag aaccacgaca acccacggtt cgcttcgtaa
1501 gtcttccctt ttattttccg ttcccaattt ccacacagaa ccccacctaa caagagcaaa
1561 gttacaccaa cgacatagcc ctcgccaaga acgtcgcagc attcatcatc ctcaacgacg
1621 gaatccccat catctacgcc ggccaagaac agcactacgc cggcggaaac gaccccgcga
1681 accgcgaagc aacctggctc tcgggctacc cgaccgacag cgagctgtac aagttaattg
1741 cctccgcgaa cgcaatccgg aactatgcca ttagcaaaga tacaggattc gtgacctaca
1801 aggtaagcac aacctctaag cataccctaa tggcctatct tcagagtatc tgacacaaga
1861 gactaatcac tggcaataca gaactggccc atctacaaag acgacacaac gatcgccatg
1921 cgcaagggca cagatgggtc gcagatcgtg actatcttgt ccaacaaggg tgcttcgggt
1981 gattcgtata ccctctcctt gagtggtgcg ggttacacag ccggccagca attgacggag
2041 gtcattggct gcacgaccgt gacggtgggt tcggatggaa atgtgcctgt tcctatggca
2101 ggtgggctac ctagggtatt gtatccgact gagaagttgg caggtagcaa gatctgtagt
2161 agctcgtgaa gggtggagag tatatgatgg tactgctatt caatctggca ttggacagtg
2221 agtttgagtt tgatgtacag ttggagtcgt tactgctgtc atccccttat actcttcgat
2281 tgtttttcga accctaatgc caagcacgct agtctattat aggaaaggat ccccggg
//