GenBank-Updates@genbank.bio.net (03/05/91)
LOCUS BBRGS1 4528 bp ds-DNA BCT 05-MAR-1991
DEFINITION Bacillus brevis gramicidin S synthetase 1 gene, complete cds.
ACCESSION D00519
KEYWORDS gramicidin S synthetase 1.
SOURCE Bacillus brevis chromosomal DNA, clone pGS309.
ORGANISM Bacillus brevis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 4528)
AUTHORS Hori,K., Yamamoto,Y., Minetoki,T., Kurotsu,T., Kanda,M., Miura,S.,
Okamura,K., Furuyama,J. and Saito,Y.
TITLE Molecular cloning and nucleotide sequence of the gramicidin S
synthetase 1 gene
JOURNAL J. Biochem. 106, 639-645 (1989)
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Kazuko Hori
Dep.of Biochemistry and Dep. of Genetics
Hyogo college of Medicine
1-1 Mukogawa-cho, Nishinomiya,
Hyogo 663
Japan
0798-45-6357
FEATURES Location/Qualifiers
misc_binding 558..562
/note="ribosome-binding site (putative)"
CDS 568..3864
/note="gramicidin S synthetase 1"
BASE COUNT 1573 a 700 c 921 g 1334 t
ORIGIN 947 bp upstream of EcoRI site.
1 agctgaggaa atacaaccat taataaatat tccatttgct ttttttgggc atagcatggg
61 agcattaata agttttgaac tggctcgcac aatacgacaa aagagtaatg ttaatccggt
121 tcacttgttt gtttcagggc gacatgcacc tcaaatccca tgtgcaaaac aagactatca
181 tttacttccc gatgaacaat ttatacaaga attgcgttca ttgaatggaa ctccagagat
241 agtattacaa gacgcagaga tgatgagtat attactccca agacttcggg ctgatttttc
301 tgtgtgtggc tcctatcagt acaaaaacga cgagcctttt gaatgcccaa tcactgcttt
361 tggaggaaaa aatgataatg gtgttactta tcaatcatta gaagcctgga gagagcaaac
421 caagagggaa ttttctgtgt gtatgtatcc aggtgatcat ttttttcttt acgaaagcaa
481 atatgaaatg attgagttca tgtgtaaaca attacgttta gtattagctc ctaaaatata
541 aataactatt ttttacaggg ggtatatatg ttaaacagtt ctaaaagtat attgattcat
601 gctcaaaata aaaatggaac gcatgaagag gagcagtatc tctttgctgt gaacaacacc
661 aaagcggagt atccacgtga taagacgatc catcagttat ttgaagagca ggttagtaag
721 agaccaaaca atgtagccat tgtatgtgaa aatgagcaac ttacctacca tgagcttaat
781 gtgaaagcca atcaactagc acggattttt atagaaaaag ggattggaaa agacactctt
841 gttggaatta tgatggagaa atctatcgat ttatttatag gcatattagc cgttttaaaa
901 gcaggtggag catatgttcc gattgatatt gaatatccta aggaaagaat tcaatatatt
961 cttgatgata gtcaggcaag aatgctactt actcagaagc atttggttca tttaattcat
1021 aatattcaat ttaatgggca agtggaaatt tttgaagaag atactatcaa aattagagaa
1081 ggaactaatc tacatgtacc aagtaaatca accgatcttg cttatgttat ttatacttct
1141 ggtacaacag gcaatccaaa aggtacaatg ctggagcata aaggaataag taatctaaag
1201 gtatttttcg aaaatagtct taacgtgact gaaaaggata gaattggtca atttgccagc
1261 atctcttttg atgcatctgt atgggagatg tttatggctt tgttaacggg ggctagcctg
1321 tacattatcc tgaaggatac aatcaatgat tttgtgaagt ttgaacaata cattaaccaa
1381 aaggaaatca ctgttattac gttaccacct acctatgtag ttcatcttga tccagaacgt
1441 attttatcga tacaaacgtt aattacagca ggctcagcta cctcgccttc cttagtaaac
1501 aagtggaagg agaaagtaac ttacataaat gcctatggcc ctacggaaac aactatttgt
1561 gcgactacat gggtagccac caaagaaaca acaggtcatt cagttccaat cggagcacca
1621 attcaaaata cacaaattta tattgtcgat gaaaatcttc aattaaaatc ggttggtgaa
1681 gctggtgaat tgtgtattgg tggagaaggg ttagcaaggg gatattggaa gcgaccggaa
1741 ttaacttccc agaagttcgt tgataacccg tttgttccag gagagaagtt gtataaaaca
1801 ggagatcagg caagatggct acctgatgga aatattgaat atctcggaag aatagataac
1861 caggtaaaga ttagaggtca ccgagttgaa ctagaagaag ttgagtctat tcttctaaag
1921 catatgtata ttagcgaaac tgcagtaagt gtgcataaag atcaccaaga acagccgtat
1981 ttgtgcgcta tatttgtatc ggaaaagcat ataccactag aacagttaag acaattctca
2041 tcagaagaac tgccaacgta tatgatccct tcttatttta tccagttaga caaaatgcct
2101 cttacatcaa atgggaagat tgatcgaaag cagttaccgg aacctgattt aactttcggg
2161 atgagggtag actatgaagc gccgcgaaat gaaatcgagg aaacgcttgt tactatctgg
2221 caggatgtat taggtattga gaaaatcggt attaaagata atttctatgc attaggtgga
2281 gattctatta aagcaataca ggttgctgct cgcctgcatt cctaccaatt aaagctagaa
2341 acaaaagatt tattaaagta tccaacaatc gatcaactcg ttcattatat aaaagatagt
2401 aaaagaagaa gtgagcaagg tattgtggaa ggtgagattg gacttacacc tattcagcat
2461 tggttctttg aacaacaatt tacaaatatg caccattgga accaatcgta tatgttgtat
2521 agaccaaatg ggtttgataa agagatcttg ctaagggtat ttaataaaat tgttgagcat
2581 catgatgcat tacgtatgat atacaaacat cataacggaa agatcgtgca gataaatcgg
2641 gggcttgaag gtacgttgtt tgatttttat acctttgatt taactgcaaa tgataatgag
2701 caacaggtga tttgtgaaga atctgctcga ttacaaaata gtataaactt ggaagtaggc
2761 cctctagtaa agatagcgct gtttcatact cagaatggag atcacctgtt tatggctatt
2821 catcatttgg ttgtggatgg tatttcttgg aggattttgt ttgaggattt ggccacagct
2881 tatgaacaag cgatgcatca gcaaacgatt gctttaccag agaaaacaga ttcatttaag
2941 gactggtcta ttgaattaga aaaatatgct aacagcgaat tattcctgga agaggctgaa
3001 tattggcatc atttgaatta ttataccgat aacgttcaaa ttaagaaaga ttatgtcacc
3061 atgaacaata aacaaaagaa tatacgttat gtaggaatgg agttaacaat agaagagaca
3121 gaaaaattat tgaaaaatgt aaataaagcg tatcgaacag aaattaatga tattttatta
3181 acggcacttg gctttgcact caaagaatgg gccgatattg ataaaattgt aattaactta
3241 gagggacacg gacgggaaga aatactggaa cagatgaaca ttgcaaggac ggtaggctgg
3301 tttacttccc agtatcctgt tgtacttgat atgcaaaaat cggatgattt gtcttatcaa
3361 atcaaattaa tgaaagaaaa tttacgcaga atacctaaca aaggaatcgg atatgaaatt
3421 tttaagtatt taacaactga atatttacgg cctgttttac cctttacatt aaagccggaa
3481 attaacttta actacttagg acagttcgat acggacgtga agactgaatt gtttactcgt
3541 tctccttata gcatgggtaa ttcattagga ccagatggaa aaaataattt aagcccagaa
3601 ggggaaagtt attttgtact caatattaat ggttttattg aagaaggtaa gcttcacatc
3661 accttttctt ataatgaaca gcagtataag gaggatacca ttcagcaatt gagccggagc
3721 tataagcaac atcttttggc catcattgaa cattgtgtac agaaggaaga tactgagtta
3781 actccaagtg atttcagttt caaggaactt gaattagaag agatggatga tattttcgat
3841 ttgttggccg attcattaac gtaaatataa ccaactaaat ccatggtttt ttaatgataa
3901 atgctttgaa aattcattat tatgaggtgc tagcatgagt acatttaaaa aagaacatgt
3961 tcaggatatg tatcgtttat ctcccatgca ggaaggcatg ttgtttcacg cattacttga
4021 taaagataaa aatgctcacc tggtacaaat gtctatcgcg atcgaaggta tcgtggatgt
4081 ggagctgctt agtgaaagct tgaacatatt gattgataga tacgatgtgt ttagaacaac
4141 attcttacat gaaaaaatta aacaaccgct tcaggtagtg ctaaaggaac ggcctgttca
4201 gcttcaattt aaagacatat catccttaga tgaagaaaaa agagaacagg ctattgagca
4261 gtataagtat caagatgggg aaacagtctt tgatttaaca agagatccct tgatgagagt
4321 agctattttt caaactggta aggttaacta ccaaatgatc tggagcttcc accatatttt
4381 aatggatggt tggtgcttca acattatatt taatgacttg ttcaatatat atctgtcatt
4441 aaaagagaag aaacctcttc agttagaggc ggtgcaacca tataagcagt ttattaagtg
4501 gcttgaaaaa caagataaac aggaagct
//