GenBank-Updates@genbank.bio.net (03/05/91)
LOCUS BCEGLNA 2352 bp ds-DNA BCT 05-MAR-1991
DEFINITION Bacillus cereus glutamine synthetase (glnA) gene.
ACCESSION D00513
KEYWORDS glnA gene; glutamine synthetase.
SOURCE Bacillus cereus IFO3131 chromosomal DNA, clone pCGS1.
ORGANISM Bacillus cereus
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 2352)
AUTHORS Nakano,Y., Kato,C., Tanaka,E., Kimura,K. and Horikoshi,K.
TITLE Nucleotide sequence of the glutamine synthetase gene (glnA) and its
upstream region from Bacillus cereus
JOURNAL J. Biochem. 106, 209-215 (1989)
STANDARD full staff_entry
COMMENT DNA was sequenced by the dideoxy-chain termination method of Sanger
et al., using the Automated DNA Sequencer System (Applied
Biosystems).
FEATURES Location/Qualifiers
misc_signal 250..271
/note="putative transcriptional terminator"
misc_signal 359..364
/note="putative -35 promoter sequence"
misc_signal 380..385
/note="putative -10 promoter sequence"
misc_signal 405..411
/note="SD sequence"
misc_signal 845..851
/note="SD sequence"
CDS 860..2194
/note="glutamine synthetase"
misc_signal 2209..2234
/note="putative transcriptional terminator"
BASE COUNT 795 a 391 c 484 g 682 t
ORIGIN 4 bp upstream of EcoT22I site.
1 atgcatcccc gattaattct cacttcactc catatgctaa ctatatgccg gggtatgaag
61 atgatgtaat tatggctgcg ggaactttta ttcaaggtgc aagtattgaa ttgtcagctg
121 atggtccaat tcgtccgcct tatgttgctt acgtacaagg tgggctaact tattcgcatg
181 tgaagattgc gatctgttct gcaattgatg cattaattga aaaagaatta ttaacaattt
241 cttaaaggga agctgtcgat ttcgacagct ttttttcgtg tgaaagtaat gatttattgc
301 attagaaaaa agaatgaaaa aacttttaaa aaaatcatgt aagaaaagct aacatctatt
361 gacatcaaac ataacatggt ataaaatgag aagcagttaa ggcgaaggag gaactgaaac
421 gatgaaagaa gatagacgtt ctgccccgct gtttcctatt ggtattgtta tggatttaac
481 acaattatct gcacgtcaaa ttcgctacta tgaagagcat aatcttgttt ctccaacccg
541 tacaaagggg aatcgtagat tattttcatt taacgatgta gataagttgt tagagattaa
601 agatttatta gatcaaggct tgaatatggc tggtattaaa caagtgttac taatgaaaga
661 aaatcaaaca gaagcagtga aagtaaaaga agaaacgaaa gaaatttcaa aaactgagct
721 tcgcaaaata cttcgagacg aactacaaca tacaggtaga tttaatcgaa cttcattgcg
781 acaaggtgat atttcaaggt tttttcacta aagagaactg attctgaagg tgtttagagg
841 gactaaggag gaatttataa tggctaggta cacaaaagaa gatattttcc gtttggcgaa
901 agaagagaat gtaaagtata tacgtttaca atttacggac cttttaggag taattaagaa
961 cgtagaaatt ccagtgagcc aattaacaaa agctcttgat aacaaaatga tgtttgatgg
1021 atcttctatt gaaggtttcg tacgtattga agaatctgat atgtacttat atccagactt
1081 agatacttgg gtgattttcc cttggacagc tgaaaaaggt aaagtagctc gattgatctg
1141 tgatatttac aatgcagatg gcactccatt tgaaggggat ccacgtaaca acttaaaacg
1201 tgtattaaaa gaaatggaag ctttaggatt ctcagatttc aatcttggac cagagccaga
1261 attcttccta ttcaaagtag atgaaaaagg aaatccaaca ttagaattaa acgataacgg
1321 tggatacttc gaccttgcgc cgatggatct aggggaaaac tgtcgtcgtg atatcgttct
1381 tgaacttgaa gaaatgggct ttgaaattga agcgtctcac catgaggttg ctccagggca
1441 gcacgaaatt gacttcaaat atgcaaatgc aattcgttca tgtgatgaca ttcaaacatt
1501 caaacttgtt gtaaaaacaa ttgctcgtaa acacggttta cacgcaacat ttatgccaaa
1561 accattatac ggtgtgaacg gttcaggtat gcactgtaac ttatcactat tcaaaaatgg
1621 tgagaacgta ttctatgatc aaaacggtga cttacaatta agtgatgatg ctcgtcactt
1681 catcgcaggt attttaaaac acgcaccagc atttacagcg gtggcaaacc caactgtaaa
1741 ctcttacaag cgtttagtac ctggatacga agctccttgt tacgtagcat ggtctgcaca
1801 aaaccgtagt ccattagtac gtatccctgc atctcgtggt attagtacac gcgtagaagt
1861 acgtagtgtt gacccagctg caaacccata tttagtaatg gctacattat tagcagcagg
1921 tcttgatgga attaaaaaca aattaactcc accagctgca gtagatcgta acatctacgt
1981 aatgacaaaa gaagagcgcg aagaagcagg tatcgttgac ttaccagcaa cattagcgca
2041 agctttagtt acattacaat ctaacgaagt aattagtaac gcattaggag atcacttact
2101 tgagcacttc atcgaagcga aagaatttga gtgggatatc ttccgtacgc aagttcacca
2161 atgggaacgc gatcaatata tgtctcttta ctaagaggtg aggtgaggag ccctgatact
2221 acggtatcag ggctttttta tttgttggca atataggtgt tagttgaaat gaattgaata
2281 gatgtctgaa aaaattatga cggtatttaa atttctattc catatcttta atgtaacgtg
2341 gtgaaattcg aa
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