GenBank-Updates@genbank.bio.net (03/05/91)
LOCUS BSTTRPAB 2529 bp ds-DNA BCT 05-MAR-1991
DEFINITION Bacillus stearothermophilus tryptophan synthase gene, complete cds.
ACCESSION D00539
KEYWORDS tryptophan synthase.
SOURCE Bacillus stearothermophilus IFO13737, chromosomal DNA, clone
pISY21.
ORGANISM Bacillus stearothermophilus
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 2529)
AUTHORS Ishiwata,K., Yoshino,S., Iwamori,S., Suzuki,T. and Makiguchi,N.
TITLE Cloning and sequencing of Bacillus stearothermophilus tryptophan
synthase genes
JOURNAL Agric. Biol. Chem. 53, 2941-2948 (1989)
STANDARD full staff_entry
COMMENT The 5' proximal portion of the trpB gene was found to overlap 20
nucleotides of the upstream coding region of the trpA gene.
FEATURES Location/Qualifiers
misc_signal 161..169
/note="SD sequence"
CDS 179..1393
/note="tryptophan synthase, beta-subunit (trpB)"
misc_signal 1363..1370
/note="SD sequence"
CDS 1374..2183
/note="tryptophan synthase, alpha-subunit (trpA)"
BASE COUNT 562 a 668 c 775 g 524 t
ORIGIN 178 bp upstream of the initiation codon of trpB gene.
1 atcagcagcg gcattgaaac ggacgggcgc aaagatccga aaaagatgaa acagattgaa
61 gaaaaaataa catattgggc taaagaaaca aaggatgttt tcgaaaccgc agcgaacaca
121 tgcccagaca acgttggagc atgtttcacg tttggaaaca agaaggtggg gatgagtgat
181 ggaacgcgtt ccgaatgaac atgggcgatt tggcgatttc ggcggcaagt ttgtcccgga
241 gacgctcatg cttccgcttg aggaaatcga agccgagctt gacaaagcgc tggcggacga
301 atcgttcaaa caagaatata tccgcatttt gcagcactac tccggtcggc cgactccgct
361 cacgttcgcg ccgaatttga cacggcagct tggtggcgcg aaaatgtatt taaagcgcga
421 agatttaaac cataccggcg cccataaaat caacaatgcc atcggccagg cgcttttggc
481 gaaacggatg ggcaagaaaa agctgatcgc ggaaaccggc gccggccaac atggggtggc
541 ggccgcgacc gtggcggccc atttcgggat ggactgcatc gtctttatgg gagaggaaga
601 catcaagcgg caagagttga acgtatttcg catgaagctg cttggcgcgg aagtggtgcc
661 ggtctcaagc ggcaaccgga cgttgaaaga cgcgacaaac gaggcgattc gctattgggt
721 cgcccattgc gacgaccatt tttacatgat cggctcggtg gtcggcccgc acccgtaccc
781 gaaaatggtg cgcgagtttc aacgcatcat tggcgatgag gcgaaagagc agtttctcgc
841 ctgcgaaggg aagctgcccg atgtcatcgt cgcctgcgtt ggcggcggca gcaacgccat
901 cggcatgttt tacccgtttt tgcaagatga cgtccgcctt gtcggggtgg aagccgccgg
961 caaaggcatt gacacccctt accatgccgc gacgatcacg aaagggacga aaggggtcat
1021 ccacggggcg atgacgtact tgctgcagga tgagtacggg caaattgtcg agccgtactc
1081 gatctcagcc ggcctcgatt accccggcgt cggtccggag catgcctatt tagcgagcat
1141 cggccgcgtc cgctacgaaa gcgtgaccga cgaggaagcg gtcgcggcgt ttcggctgct
1201 ggcgcaaaca gaaggcatca ttccggcgat tgagtcggcc catgcggtgg cgaaagccgt
1261 ggagctcgcc caatcgatgt cgccggatga aacggtgctc atttgcctgt ccggccgcgg
1321 cgataaagac gtgcaaacga tgatgcgcca tcttggcgcg aaagaaggtg aagatgttgc
1381 tgctatccgt taatccgccg ctgtttattc cctttattgt cgccggcgac ccgtcgcctg
1441 aggtgacggt ggatttggcc ttggcgcttg aggaggccgg cgccgatcta ttggagcttg
1501 gcgtgccgta ctccgacccg ctcgccgacg gaccgacgat ccagcgcgcc gccgcccggg
1561 cgcttgccgg gaacatgacg ttgccgaaag ccattcatct cgtcgccgaa atgcgaaaaa
1621 agggggtaac cattccgatt attctcttta cgtattacaa tcctgtgtta caattaggag
1681 aagaatcctt ttttgcttta gcgcgggaaa atggcgccaa cggcgtgctc attcccgatt
1741 tgccgtttga agaaagcggt ccgctccgcg aactgggcga gcggtttgac cttccgctca
1801 tttcgctcgt cgcgccgacg tcaaagcagc ggattgagcg gatcgcttcg gtagcgcaag
1861 ggtttttgta ttgcgtttcc tcgcttggcg tcaccggtat gcgcgaaacg ttgccggagt
1921 cgcttggcga ttttgtcagt gaagtcaagc ggcatagccg tgtgccggtc gctgtcgggt
1981 tcggcatctc cacgcccgaa caagtggcga tgctgaaaga ggtgtgcgat ggcgtcgtca
2041 tcggcagcgc ccttgtgcaa aaagtggaac agttggggga acggctgctg gcgccggaag
2101 aaaaagaagc ggccatcgcc gagtttgccg cctacgcccg ctcgctcgcc gcgccgcttc
2161 acgcgccgtg ttctttgcgc taaaagttcc gatgatgaag gagttggcaa cgcgaatgca
2221 agtgaaagag cagcttcggg gacttccccc ataccagccg ggaaaatcga ttgaggaagt
2281 gaagcgggaa tacggccttt ctgattatta aactagcttc caatgaaaat ccgtatgggt
2341 cgtcgtcgcc ggcggccaaa gcggccatcg ccgccgagct tgaccgtctt gccgtttatc
2401 cggacggcta cgcccgctac gctgcgcgaa aaggtggcaa cgcatcttgg cgtcaaggaa
2461 acgcagcttt tgtttggcaa cggctcggat gaagttgtgc aaattttttg ccgcgccttt
2521 ttggagccg
//