GenBank-Updates@genbank.bio.net (03/05/91)
LOCUS BSUBGLU 1087 bp ds-DNA BCT 05-MAR-1991
DEFINITION B.subtilis beta-glucanase (EC 3.2.1.73) gene, complete cds and
flanks.
ACCESSION D00518
KEYWORDS beta-glucanase; glycosidase; hydrolase.
SOURCE Bacillus subtilis (strain Y-25) DNA, clone pLE100.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 24)
AUTHORS Tezuka,H.
JOURNAL Unpublished (1989) Central Research Laboratories, Asahi Breweries
Ltd. Tokyo 143
STANDARD full staff_entry
REFERENCE 2 (bases 25 to 1087)
AUTHORS Tezuka,H., Yuuki,T. and Yabuuchi,S.
TITLE Construction of a beta-glucanase hyperproducing Bacillus subtilis
using the cloned beta-glucanase gene and a multi-copy plasmid
JOURNAL Agric. Biol. Chem. 53, 2335-2339 (1989)
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Hidetoshi Tezuka
Central Research Laboratories
Asahi Breweries Ltd.
2-13-1 Omori-kita
Ota-ku Tokyo 143
Japan
Phone: 03-763-9634
Fax: 03-765-4080
This DNA sequence showed differences in 26 nucleotides compared
with that of B. subtilis C-120 determined by Murphy et al.
(discussed in [Agric. Biol. Chem. 53, 2335-2339 (1989)]).
FEATURES Location/Qualifiers
misc_signal 189..194
/note="-35 region for sigma-43 RNA polymerase"
misc_signal 213..218
/note="-10 region for sigma-43 RNA polymerase"
misc_binding 248..255
/note="ribosome binding site (SD)"
CDS 265..993
/note="beta-glucanase precursor"
sig_peptide 265..348
/note="beta-glucanase signal peptide (putative)"
mat_peptide 349..990
/note="beta-glucanase (putative)"
repeat_region 1006..1016
/note="palindromic sequence"
repeat_region 1025..1035
/note="palindromic sequence"
BASE COUNT 335 a 191 c 236 g 325 t
ORIGIN
1 tcattttttc ttacattcac atatgaaaat ggcaggattg ttactgataa agcaggcaaa
61 acctaaatta caatgagtgc ggatcatctc tgtctgtgct gatggtaatt taggttttta
121 cttttttcag agggaagatg atgatagtta caggattcaa gtaagtaaga ttcggtatta
181 tcattatttt ggccgatgtt cccttttaaa agaatcatgt aagatcaaca tagaaaacgc
241 tttcaatgaa aggggaatgc caatatgcct tatctgaaac gagtgttgct gcttcttgtc
301 actggattgt ttatgagttt gtttgcagtc acttctactg cctcggctca aacaggtgga
361 tcgttttttg acccgtttaa cggctataac tccggttttt ggcaaaaagc agatggttat
421 tcgaatggaa atatgttcaa ctgcacgtgg cgggctaata acgtatcaat gacgtcattg
481 ggtgaaatgc gtttagcgct aacaagccca tcttataaca agtttgactg cggggaaaac
541 cgttctgttc aaacatatgg ctatggactt tatgaagtca gaatgaaacc agctaaaaac
601 acagggatcg tttcatcgtt cttcacttac acaggtccaa cagatggaac tccttgggat
661 gagattgata tcgaattttt aggaaaagat acaacaaagg ttcaatttaa ctattataca
721 aatggtgcag gaaaccatga gaagattgtg gatctcgggt ttgatgcagc caatgcctat
781 catacgtatg cattcgattg gcagccaaac tctattaaat ggtatgtcga tgggcaatta
841 aaacatactg caacaaacca aattccgaca acacctggaa agatcatgat gaacttatgg
901 aatggcacgg gtgtcgatga atggcttggc tcctacaatg gtgtaaatcc gctatacgct
961 cattatgatt gggtgcgcta tacaaaaaaa taatgccaaa ggtgaaagag cctgctgcaa
1021 atatagcagg ctcttatgat tgtaatgaga agttgttggc gtgaatcttc tattcactca
1081 taatatc
//