GenBank-Updates@genbank.bio.net (03/05/91)
LOCUS GFLRNA3 1114 bp ss-RNA VRL 05-MAR-1991 DEFINITION Grapevine fanleaf virus, satellite RNA (RNA3), complete sequence. ACCESSION D00442 KEYWORDS satellite RNA. SOURCE Grapevine fanleaf virus (strain F13), satellite RNA (RNA3), cDNA to RNA3, clone pA4 and pA5. ORGANISM Grapevine fanleaf virus Viridae; ss-RNA nonenveloped viruses; Isometric ss-RNA viruses; Nepovirus. REFERENCE 1 (bases 1 to 1114) AUTHORS Fuchs,M., Pinck,M., Serghini,M.A., Ravelonandro,M., Walter,B. and Pinck,L. TITLE The nucleotide sequence of satellite RNA in grapevine fanleaf virus, strain F13 JOURNAL J. Gen. Virol. 70, 955-962 (1989) STANDARD full staff_entry COMMENT The satellite RNA has a polyadenylated 3' end, and probably a protein (VPg) linked to the 5' terminus, and produces 39K protein in a wheatgerm translation system. Furthermore there is the weaker upper band. It may be readthrough product of 39K protein. FEATURES Location/Qualifiers misc_feature 3..10 /note="consensus sequence at the 5' end of nepovirus RNAs" CDS 15..1040 /note="39K product, P3" variation 720..720 /note="t in clone pA5; c in clone pA4" BASE COUNT 246 a 295 c 313 g 260 t ORIGIN 5' end of satellite RNA. 1 tatgaaaaat ttctatggac tcttacgtta ccgtggatcc atcttttcac tctcccagaa 61 tctcacttga gattctggtc cctaccaagt atgcgaagct gtttaccctt aagcagctct 121 ctcgcatgct tgcattgtcg tgtaagcacc gtgcacggca agccgctaac ccggtaagca 181 aacggacctc ccgagaccga aatgggagta aaacaatggg tcagggtcct tctgctgtgg 241 ccccgcaagt gtcgaaggga cacaatcagc aagtagatgg aggggtttgt ctagccccgg 301 tgaagtcaaa gcgtgcggta cggcgtgaaa agcgccgtac tgccgctaag aaggccacta 361 ataaggccaa gaccgagacc aaactcgtta aaaagggtgg gtctagtatc cacgccccaa 421 aggcaccgaa gcggacgtcg tatttgtcta gtctgctttc gagtccttct ggggcgaaag 481 ccaaaatggg ggctctgagc aagccccccc aaacaaaaaa tgctcccgac gccaacgagg 541 gtggtttcac cctcactgct atcacaccgg ctgagtgccg ggcggaagct aggcgcagat 601 tccaccccat cacaggatcc tctcgtggtc cttatgggtt ctgcacccgc tcccgtgagg 661 gttgtggcgt gtgtgccgat tgtgtggaaa agaaggcaca cctggacttc aaccggagtt 721 ttgacactat tggcacttcg cgtgtcattc gtgtcgactc gatgatggaa gaagtggccg 781 aagacttggc cagccctagt gtcctagaac cctctgggtt ctgggctcct gctgaaaagc 841 aggctccttc tggagagggg cattcccgta gaaggtgcga cgtggtgaca ctcgcacgcg 901 taactccggt tctccggatg ttgcgtaagg tcgatcccac tcttgtggac aaccgacttt 961 tatgggaggc cgcgtttagg acggtctttc ctcagcgcaa gtgcgtgtat ccccacggtt 1021 gcttctgtga ccgtgggtag ggaagattgc agcgggacat cgtttgttta tagtctggcg 1081 taagctggga ttttggttgc tcattagcca actc //