GenBank-Updates@genbank.bio.net (03/05/91)
LOCUS HAMEF1A 1722 bp ss-mRNA ROD 05-MAR-1991
DEFINITION Chinese hamster elongation factor EF-1 alpha mRNA, complete cds.
ACCESSION D00522
KEYWORDS elongation factor EF-1 alpha; phosphatidylinositol.
SOURCE Chinese hamster fibroblast V79-UF cells, cDNA to mRNA, clones
lambda-EF3 and 5.
ORGANISM Cricetulus longicaudatus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Cricetidae; Cricetinae;
Cricetini.
REFERENCE 1 (bases 1 to 1722)
AUTHORS Hayashi,Y., Urade,R., Utsumi,S. and Kito,M.
TITLE Anchoring of peptide elongation factor EF-1 alpha by
phosphatidylinositol at the endoplasmic reticulum membrane
JOURNAL J. Biochem. 106, 560-563 (1989)
STANDARD full staff_entry
COMMENT The 50 kDa protein anchored at the endoplasmic reticulum membrane
in chinese hamster fibroblast cells was identical with human EF-1
alpha, except that Ser was replaced by Asp at position 222.
FEATURES Location/Qualifiers
CDS 49..1437
/note="EF-1 alpha"
misc_feature 1722..1722
/note="poly(A) site"
BASE COUNT 490 a 364 c 428 g 440 t
ORIGIN 48 bp upstream of the EF-1 alpha initiation codon.
1 ccgtcagaac gcaggtgttg tgaaaaccac cgctaattca aatccaacat gggaaaggaa
61 aagactcata tcaacatcgt tgtcattgga cacgtagatt caggcaagtc caccacaacc
121 ggccacctga tctacaaatg tggtggaatc gacaaacgaa ccatcgaaaa gtttgagaag
181 gaggctgccg agatgggaaa aggctccttc aagtatgcct gggtcttgga caaactgaaa
241 gctgagcgtg aacgtggtat cactattgac atctccctgt ggaaatttga gaccagcaaa
301 tactatgtga ccatcattga tgccccagga cacagagact ttattaaaaa catgattact
361 ggcacttctc aggctgactg tgctgtcctg attgttgctg ctggtgttgg tgaatttgag
421 gctggtatct ccaagaatgg gcagacccgt gagcatgccc ttctggctta caccctgggt
481 gtgaaacagc taattgttgg tgttaacaaa atggattcca ccgagccacc ttacagccag
541 aagagatacg aggaaattgt gaaggaagtc agcacctaca ttaagaaaat tggctacaac
601 cctgacacag tagcatttgt gccaatttct ggttggaatg gtgacaacat gctggagcct
661 agtgctaata tgccttggtt caagggatgg aaagtcaccc gtaaagatgg cagtgccagt
721 ggtaccacac tgctggaagc tttggattgt atcctgccac caactcgtcc cactgacaag
781 ccactgcgac tgcccctcca ggatgtctat aaaattggtg gtattgggac tgttcctgtg
841 ggccgagtag agactggtgt tttaaaacct ggaatggtgg ttacctttgc tccagtcaac
901 gttacaacag aagtaaagtc tgttgaaatg caccatgaag ctttgagtga agctcttcct
961 ggagacaatg tgggcttcaa tgtaaagaac gtgtctgtca aagatgtcag acgtggcaac
1021 gttgctggtg acagcaaaaa tgacccacca atggaagcag ccggcttcac tgctcaggtg
1081 attatcctga accatccagg ccaaataagt gctggctatg ctcctgtact ggattgtcac
1141 acagctcaca tagcatgcaa gtttgctgag cttaaggaga agattgatcg ccgttctggt
1201 aagaagctgg aagatggccc taaattcttg aaatctggtg atgcagccat cgttgatatg
1261 gttcctggca agcccatgtg tgttgagagc ttctctgact atcctccact gggtcgtttt
1321 gctgttcgtg acatgaggca gacagttgct gtgggtgtca tcaaagctgt ggacaagaag
1381 gctgctggag cgggcaaagt caccaagtcg gcccagaaag ctcagaaggc taaatgaata
1441 ttacccctaa cacctgccac cccagtctta atcagtggtg gaagaacggt ctcagaactg
1501 tttgtctcaa ttggccattt aagtttaata gtgaaagact ggttaatgat aacaatgcat
1561 cggaaaacct tcaggaggaa aggagaatgt tttgtggaac atttttgtgt gtgtggcagt
1621 tttaagttat tagttttcaa aatcagtact ttttaatgga aacaacttga ccaaaaatct
1681 gtcacagaat tttgagaccc attaaaatac aagtttaatg ag
//