GenBank-Updates@genbank.bio.net (03/05/91)
LOCUS NPOHIM 4200 bp ds-DNA VRL 05-MAR-1991
DEFINITION Orgyia pseudotsugata polyhedrosis virus HindIII M region.
ACCESSION D00508
KEYWORDS .
SOURCE Orgyia pseudotsugata polyhedrosis virus DNA.
ORGANISM Orgyia pseudotsugata polyhedrosis virus
Viridae; ds-DNA enveloped viruses; Baculoviridae; Baculovirus;
Nuclear polyhedrosis viruses.
REFERENCE 1 (bases 1 to 4200)
AUTHORS Gombart,A.F., Blissard,G.W. and Rohrmann,G.F.
TITLE Characterization of the genomic organization of the HindIII M
region of the multicapsid nuclear polyhedrosis virus of Orgyia
pseudotsugata reveals major differences among baculoviruses
JOURNAL J. Gen. Virol. 70, 1815-1828 (1989)
STANDARD full staff_entry
COMMENT The transcription initiation sites were determined by primer
extension analysis.
FEATURES Location/Qualifiers
misc_signal 34..38
/note="putative promoter element"
CDS 51..629
/note="ORF1"
misc_signal 622..626
/note="putative promoter element"
misc_feature 624..625
/note="mRNA start site"
CDS 641..952
/note="ORF2"
misc_feature 753..753
/note="mRNA start site"
misc_feature 772..772
/note="mRNA start site"
misc_feature 874..875
/note="mRNA start site"
misc_feature 948..949
/note="mRNA start site"
misc_signal 949..953
/note="putative promoter element"
misc_feature 953..953
/note="mRNA start site"
misc_feature 957..958
/note="mRNA start site"
CDS 995..1888
/note="ORF3"
misc_feature 1593..1593
/note="mRNA start site"
misc_feature 1614..1614
/note="mRNA start site"
misc_feature 1792..1793
/note="mRNA start site"
misc_signal 1853..1857
/note="putative promoter element"
misc_feature 1854..1855
/note="mRNA start site"
CDS 1890..2576
/note="ORF4"
misc_signal 2406..2410
/note="putative promoter element"
misc_feature 2583..2584
/note="mRNA start site"
CDS 2591..3865
/note="ORF5"
misc_signal 3842..3847
/note="polyA signal"
misc_feature 3900..3903
/note="mRNA termination site"
CDS 4085..>4200
/note="p26, amino terminus"
BASE COUNT 1006 a 1218 c 1160 g 816 t
ORIGIN 3 bp upstream of HincII site.
1 gttaacaaaa cttaatactt tctccgccct aatgtaagac ggacattgac atggcgaacg
61 ccgattcgct cgacgcccgc gcgttcagtt acgcccccga cgccagcttt gaagtgatta
121 taatcacaaa cgcgcccaac gaccacgacg ggtatctgga gctgaacgcc gcggcacgct
181 tgttggcgcc gtttcaaaaa aacatatccg ccctatggac aagcgcggcg ccgtcgcaca
241 agcttgttag aaacaacaaa aactatctgc acgtttttgg gctgtttaaa tatttgcaaa
301 actacaattt caacgctaaa ccgcacccgc ccgaatacta cactgtgaaa tcggtaattt
361 gcgaccttat agcgggcgcg cagggcaaaa cgtttgaccc gctgtgtgag atcaagacgc
421 aactgtgcgc cattcaagaa agcctgaacg aggcaatagt gacgttgaac agccacgcga
481 ccgccgagcc cgcgcccggt gcgccttgcg acgcgcgcga gttggtcgaa acgctgcatt
541 cggaatacag ccaaaagctg acgtttgcta ccgacaccat tctggacaac gtgaaaagca
601 tcaaggacct ggtgtgcctc aataagtgaa aattttagcc atgaattttt gggcggcttt
661 tagcgcgtgc ttggtggggt acctggtcta ctcgggccgc ttgaacggcg aactgcagga
721 gatcaaatct attctaataa tagcgtacga ggcggcggac aagcgttaca ggggcgtgat
781 cgacgaaatt gagtcgctca aaacggacac gtttatgatg ttgtcgaact tgcaaaacaa
841 cacaattcgc acgtgggacg ccgttgcgaa aaacggcaaa aaaattgcca atttggacga
901 gcgcgtcaac gggctgctgg ccaagcacgc ggtgcccgcg ctcgtgagat aagttattat
961 caaaactttg gctgcagcta caatatcaca caatatgacg cccaacaaca acgtaatgtt
1021 cgacgacgcg tcggtgatgt ggatcgacgc cgactacatc taccaaaact ccaaaatgcc
1081 gctgtccacg ttccaacagc tgctcttctc cattccgtcc aagcatcgca aaatgatcaa
1141 cgacatcggc aacccggcgt gcaacccgcc gtcgtgctcc tttccgccca gcaacagcac
1201 ggtcaagtac atggtggaca tttacggcgc ggcggtgctg gcgctgcgct gcccgtcgct
1261 gttctccgac cagctgctaa ccacgtttac tgctaacaac tacttgagct actgcaaccg
1321 ccaacggccg tgcccgcagc cgccgtgccc gcagccgccc ttcgactgcg cgcaaaccca
1381 aatcctggac gcgttggaaa aactggcgcg ccagagcgac ctggtcgtga acagcctcaa
1441 ccagatctca ctgaaccaat ccaaccagtt tttggaactg tccaacacgc tgaacacggt
1501 gcgcgcccaa aacgcgcaga tcttggcggc gctggaaacc accaaagacg caatcttgac
1561 gcgcctcaac gcgttggtgg acgacattaa agcggcgctg cccgaccaat ccgcccaact
1621 gcaagaattg gccgacaagc tgctggacgc aatcaactcg gtcgcgcaaa cgcttcgcgg
1681 cgaaatgaac aacaccaatt cgattctaac caatttggcg tccagcatca ccaacatcaa
1741 cagcacgttg aacaacttgc tggccgccat tgagggtata ggcggcgacg gcggcggtct
1801 tggcgacgca gataggcaag cgctaaacga ggtgctcagc ctggtcaccg aaataagaaa
1861 cattttgatg ggcaccgctc gcaagtagca tgcccagaga cacgaaaccg tacagccggc
1921 cggccaacgc gccgcgcccg ggcgttaaaa ccgagcgctc aaaccagttt aaagcggcgt
1981 caaccaaata cggcaaacgc gtcaacgacg cgaacaagga aacgcgcccc gtggcgttcg
2041 tggacatcaa gctcaaccac cgcgccacgc ccgaaatgga gcaacggatg caggccgtgt
2101 acgcgcggca aaagccgtcc atattcaaca aatccgccgt agcagagttc attaaaaatc
2161 gcacgtttgt ggtggcgttt gcgcacccca cgtacacgat acagtttaac aaaattccca
2221 ccgtcgacca gctgcgtgtc tattgttcca acattaacaa ggacttgttg ttcaaagata
2281 aaaaccaaca aatgcgaaac gcgctgccca acgcggcgtc atttcgcgtg caaattaacg
2341 acgagccgct gtttaaggtg caaaccgtcg tgtacgacaa agaatccgcc caattggtgt
2401 tgacgataag gtttttgccc agacaaacaa tggagctgct gccgatgaaa aagtgcgttg
2461 tatatctgaa catattgtcg aacgcgtctg tggattgggc agtgccggcg gatttgctgc
2521 gcgcgtttgc caagctggcg ctggcgccgc ccatccccgc gccgcagaac gtttaacgcc
2581 ccgcttagcc atgcacgcgc cgctcaccgc ggagcagcgc gacgtgtacg acaagtacaa
2641 gtttgccacg tacgcgcggt cggtggcgtt gacgcgcgcg cagctcgacc agtggcgcga
2701 caacaaggtt atcgttccgg aacccgtgtc gcgcgcagag acgctgcgcg tggaggccgc
2761 cacgcgcggc cagagcaaga acgcgctgtg gaacctgctg cggctggacc gcagcaccgc
2821 gtcgcgctcg tcgggcggcg tcgcactgcg ctcgtccgcg ctggcgtttg gcaacgcgca
2881 agagaaccgg ctaaagttgg ccaacggaga gctgttcgaa cggctgggcc ggctggccgc
2941 ggagcgcgcg ggctgccccg tcgccgagac ggtgctggac tgcggcatgt ttattagcgc
3001 gtttgggctg cactcggcgt cgcccgacgc ctatttcgct atggccgacg gctcgtgcgt
3061 gcccgtggag atcaagtgcc cgttcaacta ccgcgacacc acggtggacc aaatgcgctt
3121 ggagctgggt aaggccaacc gcaagtaccg cgtcaagcac acggcgctgc tcgtcaacaa
3181 ggcgggcccc gcgcaatttg aggtggtcaa gacgcacgac cactatcggc aaatgcaacg
3241 gcagatgtac gtgatgcgca acgcgcccgt gtgtttttac gtggtgcggt ttaaacacaa
3301 cctggtggcg ctggccgtgc cgcgcgacga cgatttttgc cgaaaagaag cggccgcgga
3361 gggcgccgcg ttcgtggctt tcgcaacgga gaacgcgggg cgcgtgcagt ttaagcgcgg
3421 cgaccgccgc cgcgcgtctt ttgcccaaaa cgccgccgac cacggataca acgccgcgca
3481 ggtggacgcg ctggtgcgcc gcggactgta tttgtcgtac gggcagttgc gctgcgggca
3541 ttgcgacgcg tttgcgctgg acggcccgcg ggcgtttgag ctggccatgg cgcgcccaca
3601 cgaacaatgc gacgggctgg cgctgcagga gcacgagttc gacaatgttg cgtttctcga
3661 tttcaccaaa cgctacacca gtttggtcga caagcgctgc gacgacgccc gcgcactgcg
3721 cgtcgacggc ttctacgtgg acgacgccgg cgccgtgaaa accttttgtt gcggcgtgca
3781 cggcagcaat gcgtcacggc gccaccttcc cacgtgttcg tattatttag caatgggtgt
3841 aaataaaata caaaataata tgtgatttat ttattggtaa taattaaaaa ctgtttgcac
3901 aaacttgatt tttatttcgg caagaaccat tcgtccatgt atttgtttga cacgttcgtt
3961 ttgcattcaa ccggtttgca cgcgtcgctg ttttgcgcgc acgcaatcac acatatttgt
4021 cacatcgcta tttaaaagga aacgcggtcg caaaagctca aacgttattt gaaaccggcg
4081 cgcaatgtac ataaagatgg aggttgaatt tgacgaagac acgggcgccc ttcaaatcgg
4141 cggacaagag gtgtttgtaa tggtgttcga gcccggtcaa gaggtttttg acaaaagctt
//