GenBank-Updates@genbank.bio.net (03/05/91)
LOCUS PLVCP 693 bp ss-RNA VRL 05-MAR-1991
DEFINITION Potato leaf roll virus 23K ORF (putative coat protein gene) and 17K
ORF.
ACCESSION D00436
KEYWORDS coat protein.
SOURCE Potato leaf roll virus, cDNA to RNA genome, clone LP79.
ORGANISM Potato leaf roll virus
Viridae; ss-RNA nonenveloped viruses; Isometric ss-RNA viruses;
Luteovirus.
REFERENCE 1 (bases 1 to 693)
AUTHORS Kawchuk,L.M., Martin,R.R., Rochon,D.M. and McPherson,J.
TITLE Identification and characterization of the potato leafroll virus
putative coat protein
JOURNAL J. Gen. Virol. 70, 783-788 (1989)
STANDARD full staff_entry
COMMENT The nucleotide sequence was determinated by the dideoxynucleotide
method of Sangar using double-stranded plasmid DNA as template.
Comparison of the potato leaf roll virus (PLRV) 23K ORF with the
barley yellow dwarf virus (BYDV) coat protein gene gave 58.0%
similarity at the nucleotide sequence and 47.1% similarity at the
amino acid sequence. This and other observations suggested that 23K
ORF encodes the PLRV coat protein. On the other hand, there is less
homology (31.1%) between the 17K ORFs of PLRV and BYDV at the amino
acid sequence.
FEATURES Location/Qualifiers
CDS 34..660
/note="23k ORF (putative coat protein)"
CDS 59..529
/note="17k ORF"
BASE COUNT 193 a 181 c 178 g 141 t
ORIGIN 613 bp upstream of BamHI site.
1 taaagatttc ctcccacgtg cgatcaattg ttaatgagta cggtcgtggt taaaggaaat
61 gtcaatggtg gtgtacaaca accaagaagg cgaagaaggc aatcccttcg caggcgcgct
121 aacagagttc agccagtggt tatggtcacg gcccctgggc aacccaggcg ccgaagacgc
181 agaagaggag gcaatcgccg ctcaagaaga actggagttc cccgaggacg aggctcaagc
241 gagacattcg tgtttacaaa ggacaacctc gtgggcaact cccaaggaag tttcaccttc
301 gggccgagtc tatcagactg tccggcattc aaggatggaa tactcaaggc ctaccatgag
361 tataagatca caagcatctt acttcagttc gtcagcgagg cctcttccac ctcctccggt
421 tccatcgctt atgagttgga cccccattgc aaagtatcat ccctccagtc ctacgtcaac
481 aagttccaaa ttacgaaggg cggcgccaaa acttatcaag cgcggatgat aaacggggta
541 gaatggcacg attcttctga ggatcagtgc cggatactgt ggaagggaaa tggaaaatct
601 tcagatcccg caggatcctt cagagtcacc atcagggtgg ctttgcaaaa ccccaaatag
661 gtagactccg gatcagagcc tggtccaagc cca
//