GenBank-Updates@genbank.bio.net (03/05/91)
LOCUS PLVGR 5987 bp ss-RNA VRL 05-MAR-1991
DEFINITION Potato leafroll luteovirus genomic RNA.
ACCESSION D00530 X14600
KEYWORDS RNA-dependent RNA polymerase; coat protein; readthrough..
SOURCE Potato leafroll luteovirus (strain 1, a field isolate obtained from
infected tubers of the potato variety Cara grown in Scotland), cDNA
to viral RNA.
ORGANISM Potato leaf roll virus
Viridae; ss-RNA nonenveloped viruses; Isometric ss-RNA viruses;
Luteovirus.
REFERENCE 1 (bases 1 to 5987)
AUTHORS Mayo,M.A., Robinson,D.J., Jolly,C.A. and Hyman,L.
TITLE Nucleotide sequence of potato leafroll luteovirus RNA
JOURNAL J. Gen. Virol. 70, 1037-1051 (1989)
STANDARD full staff_entry
COMMENT Submitted in computer readable form by M. A. Mayo on 24-Jan-1990.
In [J. Gen. Virol. 70, 1037-1051 (1989)], they suggested that the
viral polymerase was translated by
frameshift readthrough near the end of the 70K polypeptide, and 53K
protein was translated by readthrough of the amber termination
codon of the coat protein gene. In the sequence, 5' end may not be
the extreme end (discussed in [J. Gen. Virol. 70, 1037-1051
(1989)]).
FEATURES Location/Qualifiers
CDS <1645..3495
/note="ORF2b (put. RNA-dependent RNA polymerase) (see
comment)"
CDS <4320..5846
/note="ORF5 (53K protein) (see comment)"
virion 1..5987
/note="potato leafroll luteovirus genome RNA (see
comment)"
CDS 175..918
/note="ORF1 (28K protein)"
CDS 308..2227
/note="ORF2a (70K protein)"
variation 479..479
/note="t in cloned cDNA from first RNA preparation ( first
clones); c in cloned cDNA from second RNA preparation
(second clones)"
variation 521..521
/note="c in first clones; t in second clones"
variation 571..571
/note="c in first clones; t in second clones"
variation 937..937
/note="c in first clones; t in second clones"
variation 1474..1474
/note="g in first clones; a in second clones"
variation 1611..1611
/note="a in first clones; g in second clones"
variation 1755..1755
/note="t in first clones; c in second clones"
variation 1774..1774
/note="t in first clones; c in second clones"
variation 1969..1969
/note="g in first clones; a in second clones"
variation 2004..2004
/note="g in first clones; a in second clones"
variation 2020..2020
/note="a in first clones; g in second clones"
variation 2031..2031
/note="c in first clones; t in second clones"
variation 2083..2083
/note="c in first clones; a in second clones"
variation 2085..2085
/note="c in first clones; t in second clones"
variation 2118..2118
/note="c in first clones; a in second clones"
variation 2125..2125
/note="c in first clones; a in second clones"
variation 2169..2169
/note="t in first clones; g in second clones"
variation 2470..2470
/note="g in first clones; a in second clones"
variation 2565..2565
/note="g in first clones; a in second clones"
variation 2583..2583
/note="g in first clones; a in second clones"
variation 2586..2586
/note="a in first clones; g in second clones"
variation 2649..2649
/note="c in first clones; t in second clones"
variation 2898..2898
/note="t in first clones; c in second clones"
variation 3000..3000
/note="t in first clones; c in second clones"
variation 3009..3009
/note="t in first clones; c in second clones"
variation 3186..3186
/note="t in first clones; c in second clones"
variation 3282..3282
/note="c in first clones; t in second clones"
variation 3525..3525
/note="a in first clones; g in second clones"
variation 3572..3572
/note="t in first clones; a in second clones"
CDS 3693..4319
/note="ORF3 (put. coat protein)"
CDS 3718..4188
/note="ORF4 (17K protein)"
variation 3748..3748
/note="t in first clones; g in second clones"
variation 3930..3930
/note="g in first clones; a in second clones"
variation 3939..3939
/note="a in first clones; t in second clones"
variation 4501..4501
/note="t in first clones; g in second clones"
variation 4510..4510
/note="g in first clones; c in second clones"
variation 4511..4511
/note="t in first clones; a in second clones"
variation 4697..4697
/note="t in first clones; c in second clones"
variation 4817..4817
/note="t in first clones; c in second clones"
variation 4883..4883
/note="c in first clones; t in second clones"
variation 4886..4886
/note="t in first clones; c in second clones"
variation 4895..4895
/note="t in first clones; c in second clones"
variation 4898..4898
/note="c in first clones; t in second clones"
variation 5038..5038
/note="c in first clones; t in second clones"
variation 5042..5042
/note="g in first clones; a in second clones"
variation 5071..5071
/note="a in first clones; g in second clones"
variation 5199..5199
/note="c in first clones; t in second clones"
variation 5215..5215
/note="g in first clones; a in second clones"
variation 5247..5247
/note="t in first clones; c in second clones"
variation 5339..5339
/note="c in first clones; t in second clones"
variation 5341..5341
/note="c in first clones; t in second clones"
variation 5352..5352
/note="t in first clones; c in second clones"
variation 5393..5393
/note="c in first clones; t in second clones"
variation 5426..5426
/note="t in first clones; c in second clones"
variation 5477..5477
/note="c in first clones; t in second clones"
variation 5490..5490
/note="a in first clones; g in second clones"
variation 5495..5495
/note="t in first clones; c in second clones"
variation 5511..5511
/note="a in first clones; t in second clones"
variation 5741..5741
/note="t in first clones; g in second clones"
variation 5767..5767
/note="c in first clones; t in second clones"
BASE COUNT 1665 a 1514 c 1447 g 1361 t
ORIGIN 5' end of viral RNA.
1 ctttatgata ctatgcaaat tgtgctacca gaaatccgta cagtatgcgt aggattagcc
61 gcttcaatgg gatctgttat cctggtcgga ggagaaatta ccaaacgtct agcattcccg
121 agaaattgca gctttagcgc ataaactcta cactcattgc aaacgttata gcatatgatt
181 gtattgaccc agtctggaac cttgcttttt gaccaaagat ttaaactctc aaagtttctc
241 ttcgttgtca ttgcaacagg ctttcctctt ctcctgcagc aagcgagctt aatttacggc
301 tataatcatg aacagattta ccgcatatgc cgctcttttc ttcatgttct ccctttgctc
361 aactgcaaaa gaggcaggat ttctacatcc ggccttcaac ttccgaggca cctccactat
421 gagtgcctcg agtggggatt actctgcggc acccaccccg ctatacaaat cgtgggcctt
481 accatcgtca ttaaacttga cgacccaacc accgccgccg cttacagatc ggagctacta
541 cgagttagtt caagctctta catccaaaat gcggctggat tgtcaaacgg ttggggacat
601 gacatggagg catttgtcag aaatgctatt tgcctcctgg aactccgtga aagaagtatc
661 cctcaaagcg gcctccgtga ccttatgggc aattatcaac atttggttcg gtctctattg
721 gacgcttgca aggttgatca ctttgttcct ctggactttc agcatagaag ccttatgctt
781 aattttgctc ggttgtataa ccagcttgat ctacaagggc gcgctaagtc tttcagagca
841 cttaccggtt ttcctgttta tgtcccctct gaagattatt tggagggcag ctttctccaa
901 aaggaattac aagaatgaga gggctgtgga aggatacaaa gggttttcgg tcccacagaa
961 accgccaaag tctgccgtaa ttgaactaca acatgaaaac ggcagccatc tcgggtacgc
1021 gaactgcatt cgcttgtaca gtggagagaa cgccttggtg acagctgaac actgtctaga
1081 aggcgctttc gcaacgtcgt tgaaaactgg aaacaggatt ccgatgtcga ctttctttcc
1141 cattttcaaa agtgcccgta atgatatctc catactagta ggtccaccca actgggaagg
1201 tctactatca gtcaaaggag ctcatttcat tacagctgac aaaatcggca aaggtcctgc
1261 ctctttctac actcttgaga aaggggagtg gatgtgccat agtgccacca tagatggagc
1321 ccatcaccag ttcgtgtctg ttttatgcaa cactggaccc ggatattccg gaacagggtt
1381 ttggtcttca aagaatctgc ttggtgtgct taaaggcttc ccactggaag aggagtgtaa
1441 ctacaatgtt atgtctgtta taccctcgat cccgggaatc acttccccaa attatgtgtt
1501 tgagtcgacc gccgtaaaag gccgcgtctt ctcggatgaa gctgtgaaag agctagagcg
1561 ggaagcatcc gaagccgtca agaagcttgc cagatttaaa tcacttaccg acaagaactg
1621 ggctgatgat tatgactccg atgaggatta cggtctggag agagaggctg caacaaatgc
1681 gcccgcagag aaaactgctc aaacaaactc agcagagaag actgctccat caacttcagc
1741 agagaaaact gctctaacaa acaagccttt aaatgggcaa gcggcaccgt ccgccaaaac
1801 aaacggcaac tccgacatcc ccgacgccgc tacaagcgca ccaccaatgg acaaaatggt
1861 cgaacagatc atcacagcta tggtggggag aatcaatctc tcggagatag aggagaagat
1921 agtgagcagg gtgtctcaga aagccctgca gaagcccaaa caaaagaagc gcggaaggcg
1981 tggagggaag aacaagcaaa acagtttacc tcctacttca acgcaatcta caagtggggc
2041 gcccaagaag gaggctgccc cccaggcttc aggaagtgcg ggcacatccc gcgctactac
2101 cacccccgca ccagaggcga aacccagtgg gggcaaaaac tctgccaagt tcaccccgag
2161 ctggcggata aaacagcagg attcggctgg ccaaaagccg gatctgaagc tgaactccaa
2221 agcctgaatc tacaggctgc caggtggctc caacgcgcgg agtcagccac tatccctggc
2281 gcggaagcaa gaaagcgcgt gattgagaaa acagttgagg catacagaaa ttgtgtaact
2341 aacgccccac tgtgctccct taaatccaaa ctggattggg ctggctttca acaagatatc
2401 cgtgaagcag tccagtccct tgagctagac gctggtgtag gcattcccta tatcgcgtat
2461 ggcctccccg cacaccgagg atgggttgag gaccataagc ttctcccagt gctcactcag
2521 ctgacctttg accgactaca gaagatgtcg gaggccagct ttgaggatat gagcgcagaa
2581 gagctagttc aagaagggct ctgtgatcct atcagactat ttgtcaaagg agagccccac
2641 aaacagagca aactcgatga aggccgctac cgcctcatca tgtctgtttc cttggtggat
2701 caactggtag cccgggttct gttccaaaat cagaacaaaa gggaaatttc cctgtggagg
2761 tctgtgcctt ccaaacccgg ttttggcctt tcaactgaca ctcaaactgc tgaattcttg
2821 gagtgtcttc aaaaggtgtc tggagcgcca tctgtggaag aattgtgtgc aaatcacaag
2881 gagtacacgc gcccaactga ctgttccggt ttcgactggt cagtcgcgta ttggatgctg
2941 gaggatgata tggaggtgag aaatcgcctg acatttaata acacccagct caccaagcgt
3001 cttcgggctg cctggttgaa gtgcatagga aactccgtcc tgtgcctgtc cgatggcact
3061 ttacttgccc aaactgttcc cggtgtgcaa aagagcggaa gttacaatac aagttcctcc
3121 aactctagaa tccgggttat ggctgcctat cactgtggcg ccgactgggc aatggccatg
3181 ggggatgatg ctctcgaagc ccccaactcc gacctggagg agtataaaac actaggtttc
3241 aaagtcgagg taggtcgaga actcgaattc tgttcacaca tcttcagaaa tccgaccctc
3301 gccgttccgg tcaacaccaa caaaatgctt tacaagttga tccatggtta taatccggaa
3361 tgtggcaatc cagaagtgat tcaaaactat ctggctgcag tattctctgt gctgcaggaa
3421 ctccgacacg atcgtgagct cgttgccaag ctccaccagt ggttggttcc gagtgccacc
3481 acaaaagaac actgaaggag ctcactaaaa ctagccaagc atacacgagt tgcaagcatt
3541 ggaagttcaa gcctcgttac atcaaccgga ctaaatagat tataaattct tagcgggatt
3601 tgctttagga ttctcatccg caatcccatt ttcagtagcc ggtttatatt ttgtttacct
3661 aaagatttcc tcccacgtgc gatcaattgt taatgagtac ggtcgtggtt aaaggaaatg
3721 tcaatggtgg tgtacaacaa ccaagaatgc gaagaaggca atcccttcgc aggcgcgcta
3781 acagagttca gccagtggtt atggtcacgg cccctgggca acccaggcgc cgaagacgca
3841 gaagaggagg caatcgccgc tcaagaagaa ctggagttcc ccgaggacga ggctcaagcg
3901 agacattcgt gtttacaaag gacaacctcg tgggcaacac ccaaggaagt ttcaccttcg
3961 ggccgagtct atcagactgt ccggcattca aggatggaat actcaaggcc taccatgagt
4021 ataagatcac aagcatctta cttcagttcg tcagcgaggc ctcttccacc tcctccggtt
4081 ccatcgctta tgagttggac ccccattgca aagtatcatc cctccagtcc tacgtcaaca
4141 agttccaaat tacgaagggc ggcgccaaaa cttatcaagc gcggatgata aacggggtag
4201 aatggcacga ttcttctgag gatcagtgcc ggatactgtg gaagggaaat ggaaaatctt
4261 cagattccgc aggatccttc agagtcacca tcaaggtagc tttgcaaaac cccaaatagg
4321 tagactccgg atcagagcct agtccaagcc cacaaccaac acccactcca actccccaga
4381 agcacgagcg atttattgct tacgttggca tacctatgct aaccattcag gccagggaga
4441 acgacgacca aatcatattg ggttccttag ggagccaaag gatgaaatat atagaggacg
4501 agaaccagaa ttatacaaag tttagttctg agtattactc tcaatcgagc atgcaagccg
4561 tccctatgta ttacttcaat gtcccgaaag ggcaatggtc agtcgacatc agctgcgaag
4621 ggtatcaacc cactagcagc acctcggatc caaaccgggg taggagtgac gggatgatcg
4681 cgtattcaaa cgcggattcc gattattgga atgttggtga agcggatggt gtcaaaattt
4741 cgaagctacg caacgataac acctaccgcc aaggtcaccc agaacttgaa attaactcgt
4801 gtcattttcg cgagggtcaa ctccttgaac gggacgctac aattagcttc cacgttgaag
4861 cgcctactga tgggcgattc ttccttgttg gtcctgccat ccagaaaacc gcaaagtata
4921 actatactat ctcatacggt gactggacgg accgagacat ggaactgggg ctgatcaccg
4981 tggtgcttga tgaacattta gaaggcactg gttcggctaa cagagtgcgg cggcccccac
5041 gggagggcca cacctatatg gcctcgccgc acgaaccgga aggaaaaccg gttggaaata
5101 aaccaaggga cgaaaccccg atacaaacgc aggaaagaca acctgatcaa actccgtctg
5161 acgacgtatc cgatgctggt tcggtaaaca gcggcggccc aactgagtcg ctgcgattgg
5221 agttcggggt aaactcagat agtacctacg atgctacagt cgatggtaca gactggccca
5281 gaattcctcc accaaggcac ccacctgaac ctagagtttc cggcaattca agaactgtca
5341 ctgacttttc ttcgaaagcc gatctattgg agaattggga tgccgaacac ttcgaccctg
5401 gttattccaa agaagatgtc gctgctgcta ctattatagc gcacggcagt attcaagatg
5461 ggcgaagtat gttggagaag agagaggaaa atgtcaagaa caaaacctcc tcctggaagc
5521 ccccgtcact taaagcggtg agcccagcca tagccaaatt gcgctcgatt cgcaaatccc
5581 aaccccttga gggagggacc cttaataaag acgccactga tggtgtctca tctattggca
5641 gtggttctct aacaggtggc acgcttaaga ggaaggcaac tattgaagaa cgtttactgc
5701 agaccttaac aactgaacaa aggctgtggt acgagaattt taagaaaact aaccctccag
5761 ctgctaccca atggctgttt gaatatcagc cacctcccca agtagataga aacatagctg
5821 aaaagccatt ccaagggagg aaatgagtcg actcacgact taaaactgag tgtccgccgg
5881 acattaagcg gaacgaaagc cgaaaggtga ttaggctctc aacgtctgct agagaccgtc
5941 gaaagacgcg actgtgtagc caggatcctc ttacagggtt gtgtagt
//