GenBank-Updates@genbank.bio.net (03/05/91)
LOCUS POL2CG1 7434 bp ss-RNA VRL 05-MAR-1991
DEFINITION Poliovirus type 2 RNA, complete genome.
ACCESSION D00625
KEYWORDS complete genome.
SOURCE Poliovirus type 2 (strain W-2), cDNA to genome RNA, clones p1308,
and p1345.
ORGANISM Poliovirus type 2
Viridae; ss-RNA nonenveloped viruses; Isometric ss-RNA viruses;
Picornaviridae; Enterovirus.
REFERENCE 1 (bases 1 to 7434)
AUTHORS Pevear,D.C., Oh,C.K., Cunningham,L.L., Calenoff,M. and Jubelt,B.
TITLE Localization of genomic regions specific for the attenuated, mouse-
adapted poliovirus type 2 strain W-2
JOURNAL J. Gen. Virol. 71, 43-52 (1990)
STANDARD full staff_entry
COMMENT Cleavage sites within the polyprotein-coding region are assigned
based on the original poliovirus protein sequences (Kitamura et.
al., 1981).
FEATURES Location/Qualifiers
CDS 748..7365
/note="polyprotein"
mat_peptide 748..954
/note="P1-A"
mat_peptide 955..1767
/note="P1-B"
mat_peptide 1768..2481
/note="P1-C"
mat_peptide 2482..3384
/note="P1-D (VP1, major surface capsid protein)"
mat_peptide 3385..3831
/note="P2-A"
mat_peptide 3832..4122
/note="P2-B"
mat_peptide 4123..5109
/note="P2-C (nonstructural protein)"
mat_peptide 5110..5370
/note="P3-A (nonstructural protein)"
mat_peptide 5371..5436
/note="P3-B"
mat_peptide 5437..5985
/note="P3-C (protease)"
mat_peptide 5986..7362
/note="P3-D (polymerase)"
BASE COUNT 2149 a 1741 c 1730 g 1814 t
ORIGIN 5' end of viral RNA.
1 ttaaaacagc tctggggttg ttcccacccc agaggcccac gtggcggcca gtacactggt
61 atcgcggtac ctttgtacgc ctgttttata ctcccttccc ccgtaactta gaagcacaac
121 gtccaagttc aatagaaggg ggtacaaacc agtaccacca cgaacaagca cttctgttcc
181 cccggtgagg ctgtataggc tgtttccacg gctaaaagcg gctgatccgt tatccgctca
241 tgtacttcga gaagcctagt atcaccttgg aatcttcgat gcgttgcgct caacactcaa
301 ccccagagtg tagcttaggt cgatgagtct ggacgttcct caccggcgac ggtggtccag
361 gctgcgttgg cggcctacct gtggcccaaa gccacaggac gctagttgtg aacaaggtgt
421 gaagagccta ttgagctacc tgagagtcct ccggcccctg aatgcggcta atcctaacca
481 cggagcaggc agtggcaatc cagcgaccag cctgtcgtaa cgcgcaagtt cgtggcggaa
541 ccgactactt tgggtgtccg tgtttccttt tatttttaca atggctgctt atggtgacaa
601 ttattgatag ttatcataaa gcaaattgga ttggccatcc ggtgagaatt tgattattaa
661 attactctct tgttgggatt gctcctttga aatcctgtgc actcacacct attggaatta
721 cctcattgtt gagatattat taccactatg ggcgcccaag tctcatcaca gaaagttgga
781 gcccatgaga attcaaacag agcttatggt ggatccacca ttaattacac tactattaat
841 tattacaggg attctgcgag caatgccgct agtaagcagg actttgcaca agacccatcc
901 aagttcactg aacccattaa agatgttctc attaagaccg ctcccacgct aaactctcct
961 aatatcgagg cgtgtgggta tagcgacaga gtgatgcaac taaccctagg caattctacc
1021 attaccacac aggaggcggc caattctgtc gttgcatacg gccggtggcc cgagtacatc
1081 aaggattcag aagcaaatcc tgtggaccag ccaactgagc cggacgttgc cgcatgcagg
1141 ttttacacac tagacactgt tacttggcgc aaggagtcca gagggtggtg gtggaaactg
1201 cctgatgcgc taaaggatat gggattattc ggccagaaca tgttctacca ctacctcggg
1261 agggctagct atactgtgca cgtacagtgt aatgcttcaa agtttcacca gggcgccctc
1321 ggggtatttg cagttccaga aatgtgcctg gcaggcgaca gtgcaaccca catgcttaca
1381 aaatatgaga atgcaaatcc gggtgagaaa gggggtgagt tcaaagggag ttttactctg
1441 gatactaacg ctaccaaccc tgcacgcaac ttttgtcccg ttgattatct cttcgggagc
1501 cgagtactgg cgggaaatgc gtttgtttac ccacatcaga taattaatct gcgcaccaac
1561 aactgtgcca cgttggtgct gccatacgtt aattcacttt ccatagacag catgacaaaa
1621 cacaacaatt ggggaattgc tatccttccg ctggcaccac ttgactttgc caccgagtcc
1681 tccactgaga tacccattac tctaactatt gcccctatgt gttgtgaatt caatgggttg
1741 cgcaacatca ctgtacccag aactcaaggg ttgccagtct taaacactcc aggaagcaac
1801 cagtacttaa cagcagacaa ctatcaatct ccatgtgcga tacccgagtt tgatgtgaca
1861 ccacccatag acatcccggg ggaagtgcgc aacatgatgg aattggcaga gatagacact
1921 atgatacctc tcaatctgac gagccagcgc aagaacacca tggatatgta cagagtcgaa
1981 ctgaatgatg cggctcactc tgacacacca atattgtgtc tttcactgtc tccagcatca
2041 gatcctaggc tagcacacac tatgctaggt gaaatactga actactacac acactgggca
2101 gggtctttga agttcacatt tctcttctgc ggctcaatga tggccactgg taaattgcta
2161 gtgtcctatg cacctcctgg tgcgaaagcc cctgaaagcc gcaaagaagc gatgctcggc
2221 acccacgtga tctgggacat cgggttacag tcatcatgca ctatggtggt accttggatt
2281 agcaacacca catacagaca aactatcaac gatagcttca cagaaggagg gtatatcagt
2341 atgttttacc aaactagagt tgttgtgcca ttgtccactc ctagaaagat ggacatattg
2401 ggctttgtgt cagcctgcaa tgacttcagt gtgcgcctgt tgcgtgacac gacgcacata
2461 agccaagagg ttatgccaca aggattgggt gatttaattg aaggggttgt tgagggagtc
2521 acgagaaatg ctttgacacc actgacacct gtcaacaact tgcctgatac acgatctagc
2581 ggcccagccc actctaagga aacaccagcg ctgacagccg tagagacagg ggccaccaac
2641 ccattggtgc cttcagacac ggtacaaact cgtcacgtca tccaaaagcg gacgcggtcg
2701 gagtctacgg ttgagtcttt cttcgcaaga ggagcttgtg tggccattat tgaagtggat
2761 aatgatgctc caacaaggcg tgccagtaaa ttattttcag tctggaagat aacttacaag
2821 gacaccgttc agttaagacg taagttggag ttctttacat attcaaggtt tgacatggag
2881 ttcacctttg tggttacatc caattatacc gatgcaaaca atgggcacgc actgaatcaa
2941 gtttaccaga taatgtacat accacctggg gcaccgatcc ctggcaagcg gaatgattac
3001 acatggcaaa cgtcatctaa cccatcagtg ttttacactt acggggcacc tccagctaga
3061 atatcagtgc cctacgtggg cattgccaat gcatattctc atttttacga tgggtttgcc
3121 aaagtaccac tagctggcca agcctcaaca gagggtgact cgctgtatgg agcggcttca
3181 ttgaacgact tcggatcact ggctgttcga gtggtgaatg accacaaccc tacgaaactc
3241 acttcaaaaa tcagagtgta catgaaacca aagcacgtca gagtgtggtg tccgcgaccc
3301 cctcgagcag tcccatacta cggaccaggg gttgactaca aggatggact aaccccactg
3361 ccagagaaag gcttgataac ctatggtttt ggccaccaaa ataaggcagt gtacacggca
3421 ggttacaaaa tttgcaatta ccacctcgcc acccaggaag acttacaaaa tgcgataaac
3481 attatgtgga ttagagacct tttagtagtg gaatccaaag cccaaggcat agactcaatt
3541 gctagatgta actgccacac tggagtgtac tactgtgaat ccaggaggaa gtactacccg
3601 gtctcttttg tcggccccac ctttcagtac atggaagcaa atgagtacta tccagcccga
3661 taccaatccc acatgttaat tggccatggt tttgcatctc caggggactg tggtgggatt
3721 ctcaggtgcc aacatggagt aattggaatc attacagctg gaggagaagg cctagtcgct
3781 ttctcggaca tcagagatct gtacgcatac gaggtggagg ctatggagca gggagtctcc
3841 aactatattg agtcccttgg ggctgcattt gggagtggat tcacccagca aataggaaac
3901 aaaatttcag aactcactag catggtcacc agcactataa ctgagaaact actaaagaat
3961 ctcattaaaa taatttcatc ccttgttatc atcaccagaa actatgaaga cacgaccaca
4021 gtgctggcta ctcttgctct cctcggttgt gatgcgtccc catggcaatg gctaaagaag
4081 aaagcctgtg acatcttgga aatcccctac atcatgcgac agggcgatag ctggttgaag
4141 aagtttacag aggcatgcaa tgcagccaag ggattggaat gggtgtctaa taaaatatcc
4201 aaatttattg actggctcaa agagaagatc attccacagg ctagagacaa gctagagttt
4261 gttaccaaac tgaagcaact agaaatgttg gagaaccaaa ttgcaaccat tcatcaatcg
4321 tgcccaagtc aggagcatca agaaatcctg ttcaataacg tgagatggtt atccatacag
4381 tcaaggagat ttgccccgct ctatgcggtc gaggctaaga gaatacaaaa gttagagcac
4441 acgattaaca actacgtaca gttcaagagc aaacaccgta ttgaaccagt atgtttgttg
4501 gtgcacggta gcccaggcac gggcaagtca gttgccacca atttaattgc cagagcaata
4561 gcagagaagg agaacacctc cacatactca ctaccaccag atccctccca tttcgatggg
4621 tacaagcaac aaggtgtggt gatcatggat gatttgaatc agaatccaga tggagcagac
4681 atgaagctgt tttgtcagat ggtctctact gtagaattca taccaccaat ggcttcgcta
4741 gaagaaaagg gtattttgtt cacatctaat tacgttttgg cctcaaccaa ttccagtcgc
4801 atcaccccac caactgttgc gcacagcgat gccctagcca ggcgctttgc atttgacatg
4861 gacatacaaa tcatgagcga gtattctaga gatggaaaat tgaacatggc gatggcaact
4921 gaaatgtgta agaactgtca tcaaccagca aacttcaaga gatgttgccc attggtgtgt
4981 ggcaaagcca tccagctgat ggacaaatct tccagagtca gatatagtat agatcagatt
5041 actaccatga ttattaatga gaggaacaga agatcaagta tcggtaattg catggaggca
5101 cttttccaaa gtcctcttca atacaaagac ctgaaaatag acattaagac cacacctcct
5161 cctgagtgca tcaatgattt gctccacgca gttgattctc aagaggtaag agactactgt
5221 gagaagaagg gttggatagc agacatcact agtcaggtgc aaaccgaaag aaacatcaat
5281 agagcgatga ctattcttca ggcggtcacc acatttgccg cagttgctgg agtggtgtat
5341 gtgatgtaca aactctttgc agggcatcaa ggagcgtata cagggcttcc caacaagaga
5401 cccaatgtcc ccaccatcag gactgccaag gttcagggcc caggatttga ctacgcagtg
5461 gcaatggcca aaagaaacat tcttacggca actaccatta agggagagtt cacaatgctc
5521 ggagtgcatg ataatgtggc cattctacca acccacgcat caccgggtga aacaatagtc
5581 attgatggca aggaagtaga ggtactggat gctaaagccc tggaggacca ggccgggacc
5641 aacctagaaa tcaccattgt cactctcaaa agaaatgaga agttcaggga catcagacca
5701 cacatcccca ctcaaatcac tgagacgaat gatggagttt taattgtgaa cactagtaag
5761 taccccaaca tgtatgttcc tgtcggtgct gtgactgaac aggggtatct taatctcggt
5821 ggacgccaaa ctgctcgtac tttaatgtac aactttccaa cgagagcagg tcaatgtggc
5881 ggagttatca cctgcactgg caaggtcatc gggatgcatg ttggtgggaa cggttcacat
5941 gggttcgcag cagccctgaa gcgatcctat ttcactcaga gtcaaggtga aatccagtgg
6001 atgagaccat caaaagaagt gggctacccc gttattaatg ctccatctaa aactaaactg
6061 gaacccagtg cattccatta tgtgtttgaa ggtgtcaagg aaccagctgt gctcaccaaa
6121 agtgacccca gattgaagac agattttgaa gaggctatct tttccaagta cgtgggaaat
6181 aagattactg aagtagatga gtacatgaaa gaagctgtcg atcattacgc aggccagctc
6241 atgtcactag acatcaacac agaacaaatg tgccttgagg atgcaatgta tggcactgac
6301 ggtctcgaag ctctagacct cagtaccagt gctgggtatc cctatgtggc aatggggaag
6361 aagaaaagag acattttgaa taagcaaacc agagatacaa aagaaatgca aaggcttctg
6421 gacacctatg gtattaattt acctttagtc acctatgtga aagacgagct tagatccaag
6481 accaaagtgg aacagggcaa gtccaggcta attgaggcct caagtctcaa tgactctgtc
6541 gccatgagga tggcttttgg caacttgtac gcagcattcc acaagaaccc aggtgtagtg
6601 acaggatcgg ctgttggctg tgacccagat ttgttttgga gtaaaatacc agtcctcatg
6661 gaggaaaaac tctttgatta cacgggttat gatgcttcac taagccccgc ctggtttgag
6721 gctctcaaga tggttctaga gaaaattggg tttggtgata gagtggatta cattgattat
6781 ctgaatcact cgcaccatct atataaaaac aagacatatt gtgttaaggg cggtatgcca
6841 tctggctgct ctggcacctc aatttttaat tcaatgatta ataatctaat aatcaggact
6901 ctcttactga aaacctacaa gggcatagat ttagaccacc tgaagatgat agcctatggt
6961 gatgatgtaa ttgcttccta cccccatgag gttgatgcta gtctcctagc ccaatcagga
7021 aaagactatg gactaaccat gacaccagct gacaaatcag ccacctttga aacagtcaca
7081 tgggagaatg taacattctt gaaaagattc tttagagcag atgaaaagta tccctttctg
7141 gtacatccag tgatgccaat gaaagaaatt cacgaatcaa ttagatggac taaagatccc
7201 agaaacactc aggatcatgt tcgctcactg tgcttattgg cttggcacag tggcgaggaa
7261 gagtacaata aatttttagc taagattaga agtgtgccaa tcggaagagc tttactgctc
7321 cctgagtact ccacattgta ccgccgttgg ctcgactcat tttagtaacc ctacctcagt
7381 cgaattggat tgggtcatac tgttgtaggg gtaaattttt ctttaattcg gagg
//