GenBank-Updates@genbank.bio.net (03/06/91)
LOCUS LBAGAL 3660 bp ds-DNA BCT 06-MAR-1991
DEFINITION L.delbrueckii bulgaricus beta-galactosidase gene, complete cds.
ACCESSION M23530
KEYWORDS galactosidase.
SOURCE L.delbrueckii bulgaricus (strain B131) DNA.
ORGANISM Lactobacillus delbrueckii
Prokaryota; Bacteria; Firmicutes; Regular asporogenous rods;
Lactobacillaceae.
REFERENCE 1 (bases 1 to 3660)
AUTHORS Schmidt,B.F., Adams,R.M., Requadt,C., Power,S. and Mainzer,S.E.
TITLE Expression and nucleotide sequence of the Lactobacillus bulgaricus
beta-galactosidase gene cloned in Escherichia coli
JOURNAL J. Bacteriol. 171, 625-635 (1989)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [J. Bacteriol. 171,
625-635 (1989)] kindly submitted
by B.F.Schmidt, 29-MAR-1989.
FEATURES Location/Qualifiers
misc_binding 452..458
/note="ribosomal binding site"
CDS 468..3491
/note="beta-galactosidase"
/codon_start=468
misc_feature 3477..3531
/note="region of dyad symmetry"
BASE COUNT 941 a 927 c 996 g 796 t
ORIGIN 1bp upsteam of HindIII site.
1 aagcttcact ttggcagcca gtctccgggc aattaatgaa cttggacatg gttgacgacc
61 cggtctttgc cgataaaaag ttaggcgacg gctttgccct ggtgccagca gacggtaagg
121 tctacgcgcc atttgccggt actgtccgcc agctggccaa gacccggcac tcgatcgtcc
181 tggaaaatga acatggggtc ttggtcttga ttcaccttgg cctgggcacg gtcaaattaa
241 acgggactgg ctttgtcagc tatgttgaag agggcagcca ggtagaagcc ggccagcaga
301 tcctggaatt ctgggacccg gcgatcaagc aggccaagct ggacgacacg gtaatcgtga
361 ccgtcatcaa cagcgaaact ttcacaaata gccagatgct cttgccgatc ggccacagcg
421 tccaagccct ggatgatgta ttcaagttag aagggaagaa ttagaaaatg agcaataagt
481 tagtaaaaga aaaaagagtt gaccaggcag acctggcctg gctgactgac ccggaagttt
541 acgaagtcaa tacaattccc ccgcactccg accatgagtc cttccaaagc caggaagaac
601 tggaggaggg caagtccagt ttagtgcagt ccctggacgg ggactggctg attgactacg
661 ctgaaaacgg ccagggacca gtcaacttct atgcagaaga ctttgacgat agcaatttta
721 agtcagtcaa agtacccggc aacctggaac tgcaaggctt tggccagccc cagtatgtca
781 acgtccaata tccatgggac ggcagtgagg agattttccc gccccaaatt ccaagcaaaa
841 atccgctcgc ttcttatgtc agatactttg acctggatga agctttctgg gacaaggaag
901 tcagcttgaa gtttgacggg gcggcaacag ccatctatgt ctggctgaac ggccacttcg
961 tcggctacgg ggaagactcc tttaccccaa gcgagtttat ggttaccaag ttcctcaaga
1021 aagaaaataa ccgcctggca gtggctctct acaagtattc ttccgcctcc tggctggaag
1081 accaggactt ctggcgcatg tctggtttgt tcagatcagt gactcttcag gccaagccgc
1141 gtctgcactt ggaggacctt aagcttacgg ccagcttgac cgataactac caaaaaggaa
1201 agctggaagt cgaagccaat attgcctacc gcttgccaaa tgccagcttt aagctggaag
1261 tgcgggatag tgaaggtgac ttggttgctg aaaagctggg cccaatcaga agcgagcagc
1321 tggaattcac tctggctgat ttgccagtag ctgcctggag cgcggaaaag cctaaccttt
1381 accaggtccg cctgtattta taccaggcag gcagcctctt agaggttagc cggcaggaag
1441 tgggtttccg caactttgaa ctaaaagacg ggattatgta ccttaacggc cagcggatcg
1501 tcttcaaggg ggccaaccgg cacgaatttg acagtaagtt gggtcgggct atcacggaag
1561 aggatatgat ctgggacatc aagaccatga agcgaagcaa catcaatgct gtccgctgct
1621 ctcactaccc gaaccagtcc ctcttttacc ggctctgtga caagtacggc ctttacgtca
1681 ttgatgaagc taacctggaa agccacggca cctgggaaaa agtggggggg cacgaagatc
1741 ctagcttcaa tgttccaggc gatgaccagc attggctggg agccagctta tcccgggtga
1801 agaacatgat ggctcgggac aagaaccatg cttcaatcct aatctggtct ttaggcaatg
1861 agtcttacgc cggcactgtc tttgcccaaa tggctgatta cgtccggaag gctgatccga
1921 cccgggttca gcactatgaa ggggtgaccc acaaccggaa gtttgacgac gccacccaga
1981 ttgaaagccg gatgtatgct ccggccaagg taattgaaga atacttgacc aataaaccag
2041 ccaagccatt tatctcagtt gaatacgctc acgccatggg caactccgtc ggtgacctgg
2101 ccgcctacac ggccctggaa aaataccccc actaccaggg cggcttcatc tgggactgga
2161 ttgaccaagg actggaaaaa gacgggcacc tgctttatgg gggcgacttc gatgaccggc
2221 caaccgacta tgaattctgc gggaacggcc tggtctttgc tgaccggact gaatcgccga
2281 aactggctaa tgtcaaggcc ctttacgcca accttaagtt agaagtaaaa gatgggcagc
2341 tcttcctcaa aaacgacaat ttatttacca acagctcatc ttactacttc ttgactagtc
2401 ttttggtcga tggcaagttg acctaccaga gccggcctct gacctttggc ctggagcctg
2461 gcgaatccgg gacctttgcc ctgccttggc cggaagtcgc tgatgaaaaa ggggaggtcg
2521 tctaccgggt aacggcccac ttaaaagaag acttgccttg ggcggatgag ggcttcactg
2581 tggctgaagc agaagaagta gctcaaaagc tgccggaatt taagccggaa gggcggccag
2641 atttagttga ttccgactac aacctaggcc tgaaaggaaa taacttccaa attctcttct
2701 ccaaggtcaa gggctggccg gtttccctca agtatgccgg tagggaatac ttgaagcggc
2761 tgccggaatt taccttctgg cgggccctga cggacaacga ccggggagct ggttacggct
2821 atgatctggc ccggtgggaa aatgccggca agtatgcccg cttgaaagac atcagctgcg
2881 aggtcaagga agactccgtt ttggtcaaga ctgcctttac gttgcctgtc gccttaaagg
2941 gtgatttaac cgtgacctat gaagtcgatg gacggggcaa gattgctgta acagctgact
3001 tcccaggcgc ggaagaagct ggtctcttgc cagcctttgg cttgaacctg gccctgccaa
3061 aagaactgac cgattaccgc tactatggtc tgggacctaa tgagagctac ccagaccgct
3121 tggaaggtaa ttacctgggc atctaccagg gagcggtaaa aaagaacttt agcccatatc
3181 gtccgcagga aacgggcaac cggagcaagg ttcgctggta ccagctcttt gatgaaaagg
3241 gcggcttgga atttacggcc aatggggcag acttgaactt gtctgctttg ccatattctg
3301 ccgcccaaat tgaagcagcg gaccacgctt ttgaactgac taacaattac acttgggtta
3361 gagccttaag cgcccagatg ggggtcggcg gggatgactc ctgggggcag aaggtccacc
3421 cggaattctg cctggatgct caaaaagccc gccagcttcg cctggtgatt cagccccttt
3481 tactaaaata aatgctacaa ttgacttaac aggatgaaat tttagtaaaa gcaaagcgag
3541 tgaggaagat ggcaacgatc agagaagtgc caaggcagcc ggcgtgtcgc tagcgacggt
3601 ttcccgggtc ttgaactatg accagaccct gtcagtcaat gaggcaaccc ggcagatttg
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