GenBank-Updates@genbank.bio.net (03/06/91)
LOCUS LP1PLPREP 2093 bp ds-DNA BCT 06-MAR-1991
DEFINITION Plasmid pLP1 (from Lactobacillus plantarum) replication protein
(Rep) gene, complete cds.
ACCESSION M31223
KEYWORDS replication protein.
SOURCE Plasmid pLP1 L.p(from Lactobacillus plantarum (strain CCM 1904))
DNA.
ORGANISM Plasmid pLP1
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 2093)
AUTHORS Bouia,A., Bringel,F., Frey,L., Kammerer,B., Belarbi,A.,
Guyonvarch,A. and Hubert,J.-C.
TITLE Structural organization of pLP1, a cryptic plasmid from
Lactobacillus plantarum CCM 1904
JOURNAL Plasmid 22, 185-192 (1989)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Plasmid 22, 185-192
(1989)] kindly submitted
by B.Kammerer, 11-JAN-1990.
FEATURES Location/Qualifiers
misc_binding 249..253
/note="ribosome binding site"
CDS 262..1215
/note="replication protein"
/codon_start=262
repeat_region 1335..1351
/note="direct repeat A"
repeat_region 1352..1368
/note="direct repeat B"
repeat_region 1369..1385
/note="direct repeat C"
repeat_region 1386..1402
/note="direct repeat D"
repeat_region 1403..1419
/note="direct repeat E"
repeat_region 1420..1436
/note="direct repeat F"
repeat_region 1437..1453
/note="direct repeat G"
repeat_region 1456..1467
/note="direct repeat H"
repeat_region 1468..1484
/note="direct repeat I"
repeat_region 1485..1501
/note="direct repeat J"
repeat_region 1504..1520
/note="direct repeat K"
repeat_region 1521..1537
/note="direct repeat L"
repeat_region 1538..1554
/note="direct repeat M"
rep_origin 2051..2064
/note="origin of replication (put.)"
BASE COUNT 639 a 335 c 466 g 653 t
ORIGIN 1 bp upstream of HindIII site.
1 aagcttgatt aggcttaatt gggcttgtat ccattgattt tataggcttt tggtgtatta
61 ttagggttat aaattggttg aaagaaagac aaaataaaaa cccacgtgca aattcctagt
121 ttggccgctc ggaacacgtg agttgattat catttgcgat ttatagccta ttctagggga
181 aaagccctat gatgtcaagg ttataagctt attgaaaaag atagtcagct ccttcacgtg
241 gataaactgg aggagctttt tatgtcagaa atttttgaag ataaaactga aaatggcaaa
301 gttagacctt ggcgagaacg gaagattgaa aatgtgcgct atgccgaata tttggcaatc
361 ttagaattta aacgggcaca tgatgtacgg ggttgtggtg aagttttgcg ttttcgtaag
421 attggcgagc acttaaaact ttatcaaacg tggttttgtc ataaacgatt gtgtccattg
481 tgtaattgga gaaggagcat gaaaaactcg agccagttaa aacaaattat tgcggaagca
541 gttgcaagag agcctaaagg acggtttttg tttttaactt taaccgttaa aaacgctcat
601 tcagcagagg agttaaaagt gtctttaaga gctttgacta aagcctttaa taagctaact
661 cgctataaaa aagtgactaa aaatttattg ggttatttac gttcaacgga aattaccgtt
721 aatgaacaag acgggtcata taatcaacac ttgcatgtgt tgctgtttgt aaaatcaagt
781 tattttaaga attcaaataa ttatttagca caagcagaat gggcaaaatt atggcaaaaa
841 gccttgaaag ttgattatga gcctgtggtg catgtgcagg ctgttaaagc taacaaacgt
901 aaaggaactg actctttgca agctagtgcc gaagaaacgg cgaaatacga ggtaaaatca
961 gctgattata tgacggctga tgatgagcgt aatttggtgg tgattaaaaa tttggagtat
1021 gccttagctg gaacacgaca aatcagctat ggtggattat taaagcaaat taagcaagat
1081 ttgaaacttg aagatgttga gaatggtgat ttagttcatg ttggcgatga agattacacc
1141 aaagagcaaa tggaagctgc ggaagaagtt gtcgcaaaat gggattttaa taaacaaaat
1201 tattttattt ggtaaagaga atgtcaggat atgatcaacg gtaaatccgt tggcatatcc
1261 cttttttgtt gtcagcttgc tgacttctga tacaggtttt agcattactc caatttattt
1321 ggagtgtaag tgcacattat catgtagtgc gcattatcat gtagtgcgca ttatcatgta
1381 gtgcgcatta tcatgtagtg cgcattatca tgtagtgcgc attatcatgt agtgcgcatt
1441 atcatgtagt gcgcacatta tcatgtacat tatcatgtag tgcgcattat catgtagtgc
1501 gcacattatc atgtagtgcg cattatcatg tagtgcgcat tatcatgtag tgcgcactta
1561 cacacaacat gaagttgtgt tgtgctaaac ccatcaaaac ctgcatcaga tttcgcgttg
1621 ctcaaacgta actgacttgc gtcagtttgg aacattcaaa aataaataag ttcagtcgct
1681 agctccttcg aactttttta tttttgaacg ttaattttaa aggctcttat ttgcgttcta
1741 agcgatttta gctaacagtt agctatctaa ctgtctgtca acggtaaatc gacttagagg
1801 ggcttattga gccttacagg cgatattagc ccctcttgga ggctttaagg agttgataga
1861 ctagacaata ccaaaagcct gacgtcttgg aaaacaagcc cttgttttcc cgagcccagc
1921 ggcggcaagc gttacggtcc agctggttca gctggtcagt gtggctgaaa gccacggttt
1981 aaaaaaagca gttcagcggt ttttgctgat ctgctttttg gggtttaaaa acgcaatttt
2041 tggcgttttc ttcttatctt gatactatta gcaacaacta gttttttaaa atc
//