GenBank-Updates@genbank.bio.net (03/06/91)
LOCUS LBAHELJ 3364 bp ds-DNA BCT 06-MAR-1991
DEFINITION L.helveticus helveticin J genes, complete cds.
ACCESSION M59360 M30121
KEYWORDS bacteriocin; helveticin.
SOURCE L.helveticus (strain 481) DNA.
ORGANISM Lactobacillus helveticus
Prokaryota; Bacteria; Firmicutes; Regular asporogenous rods;
Lactobacillaceae.
REFERENCE 1 (bases 1 to 3364)
AUTHORS Joerger,M. and Klaenhammer,T.
TITLE Cloning, expression, and nucleotide sequence of the Lactobacillus
helveticus 481 gene encoding the bacteriocin helveticin J
JOURNAL J. Bacteriol. 172, 6339-6347 (1990)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS 1..484
/note="ORF1"
/codon_start=2
/partial
-35_signal 1391..1396
/gene="helveticin"
-10_signal 1414..1419
/gene="helveticin"
RBS 1626..1632
/gene="helveticin"
CDS 1638..1952
/note="ORF2"
/codon_start=1638
sig_peptide 1638..1706
/note="ORF2"
/codon_start=1638
mat_peptide 1707..1949
/note="ORF2"
/codon_start=1707
RBS 1971..1975
/gene="helveticin"
CDS 1983..2984
/product="helveticin"
/gene="helveticin"
/codon_start=1983
RBS 3285..3291
/gene="helveticin"
CDS 3297..3364
/note="ORF4"
/codon_start=3297
/partial
BASE COUNT 1153 a 479 c 648 g 1084 t
ORIGIN
1 gaattccaaa gatgcagatc ctatttatgt aggaaaaaac aactacaaat atgctttaac
61 gcattatgaa accttcaagg gcaagacaat tagtccagct aaggttcaaa acgttaaatt
121 tagagttgaa aagatagtca gattccacgg caaaattagt ggtgctccat tgtacttagt
181 ggcttctaag gataagaagt acagctgctg gactacgcaa gcaatgcttc aatattatta
241 cttcaatagc aaggggatgc gcggagtagt aaatccattg aagagaattg ctaatagaag
301 tgctgataag aatattatta gtctaaagaa taaacaaaat aaacgtgact ttaatgcagc
361 aatgaaggct gctaataagc ttaagggcag tcagaagaag tttgttgtaa attctttgaa
421 gcaacttaag aaagataaca atattggcgt tgaaggcgac aacttgcttt tgtttggttt
481 ttaaagtgaa aaaaatacgt taaaagaaca tagaaaaact gatgcatcta agctatgaag
541 ctaggagcat cagttttttg ttacattaga tcgtcattgt ctttaaaaag ttgcttggtt
601 gcagcggtca ttctttcggg gatgccatag ttctgcataa aggttcctgg ttcttgaatg
661 tagtcaccat ttttgcagga atagtcgtaa ataatcttaa gagaaaatag atctgcttcc
721 tcttcttccg agttttgtct tgaaaagcta ggccaatagg cgatacctga atcacctaac
781 attaagtgac caatttcgtg accaatgata aaaggcaatt cattaggatt atgccaatta
841 gtattgatta ccattttatg agcattctta aatgaaagtg ctgggtcgta tggctctcct
901 ttaaccaaga tgtatgaaag accgtgctta aaagcataat tgagtaagta ctcaattaac
961 tcttctttac ctaagtttct catttcttgt ctgcacgacc ttccttaatg tcatcttcca
1021 ttagtcctcg aatcatattg agatatctct caggaacgtt gtaaccatga tatgaataag
1081 gcttttcttc gtctagtgga atagaagttg gttgtccttt tgcaggtata ggggtaggat
1141 ccatcagttt taccttttag gtaatcgatt gaagtattta gtacttcggc aacggttcta
1201 aggcatcgcc accaggtctt ttattcttcc aattatagat agaattagta cctaacccag
1261 ccttatcatt cacttcacgt aagttcattt tatatttttt cgctaattct ttagtacgct
1321 ctaattcaat catggcaagc attctccgaa tattttaaat tattaaataa gtatttttac
1381 taaaaatgta ttgacaatat tagtctttgg acgtaatata agaactgtca acgagatatg
1441 aaagaaataa gaaaaagcct agaaacacgt gtgtaagtaa gaaaggaatc atgtgcttaa
1501 ggttatttct tataccatta tattagtata aatactaata taagtcaacg aatattagta
1561 tctagttgat aaattttatt caaagaggtg gtgccaatga ctgtgcaata aggaaaaaga
1621 gaggtgaaga agaaatcatg gatattcatg attacgttga attgatagct ttagcgtttt
1681 gggttattag tgttgtaagt gttggtatct tgagtcatgt tcattttaag aataagaggc
1741 tggaacagtt tcgtattact gctgatgatt tgatgaaaaa ctacgttggt ttgtacaaca
1801 aagaaagttt agccagcgat caaaaaatca atcggattgt caatgcagta gtagacggac
1861 tagaagctaa aggttttaaa gtggaagacc aagatgtaaa ggatattttt gcaaaggtcg
1921 caaaaattat taatgaaaat tcttctaagt aggaagatag agattttttc ggaggtttta
1981 ttatgaagca tttaaatgaa acaactaatg ttagaatttt aagtcaattt gatatggata
2041 ctggctatca agcagtagtt caaaaaggca atgtaggttc aaaatatgta tatggattac
2101 aacttcgcaa aggtgctact actatcttgc gtggttaccg tggaagtaaa attaataacc
2161 ctattcttga attatctggt caagcaggtg gtcacacaca gacatgggaa tttgctggtg
2221 atcgtaaaga cattaatggt gaagaaagag caggtcaatg gtttataggt gttaaaccat
2281 cgaaaattga aggaagcaaa attatttggg caaagcaaat tgcaagagtt gatcttagaa
2341 atcaaatggg acctcattat tcaaatactg actttcctcg attatcctac ttgaatcgcg
2401 ccggttctaa tccatttgct ggtaataaga tgacgcatgc cgaagccgca gtatcacctg
2461 attatactaa gtttttaatt gctactgttg aaaataactg tattggtcat tttactatat
2521 acaatttaga tacaattaat gaaaaacttg atgaaaaggg aaatagtgaa gatgttaatc
2581 tcgaaactgt taaatacgaa gatagtttta tcattgataa tttatatggt gatgataata
2641 attctattgt aaattcaatt caagggtatg atttggataa tgatggaaat atttatattt
2701 ccagtcaaaa agcgccagat tttgatggct cttattatgc acatcataag cagattgtta
2761 agattccata ttatgctcgg tctaaagaaa gcgaagacca atggagagct gtaaatttaa
2821 gcgaattcgg tggcttggat attccaggta aacatagtga agttgaaagc atccaaatta
2881 ttggtgagaa tcattgttac ttaactgttg catatcattc taaaaataaa gcgggtgaaa
2941 ataaaactac tttgaatgag atttatgaat tatcttggaa ttagattctt gttagtggtc
3001 tcgatttaga tataaactaa caaaagcgga tgaaatattc attattgaaa ttcatcgctt
3061 tttattttta attaaattat tggatatact tataatatat attgctggat atattgctgg
3121 gataagagta aaataattat aggcattatt tctaaattaa aaggacaatt attatgataa
3181 aaaacaagat tatatcagct tcaattgcag cattaatggc tgtaagtcca gtgcttccac
3241 ttagctcaca ggctcatacg gttcaagctg cagataattc tgtcagaaag acagttatgc
3301 ataattcaat tgcttatgat aaagatggca attcaacagg tcaaaagtat tacgcttacg
3361 gatc
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