GenBank-Updates@genbank.bio.net (03/06/91)
LOCUS ATUCBG1 3710 bp ds-DNA BCT 06-MAR-1991 DEFINITION A.tumefaciens beta-D-glucosidase (cbg-1) gene, complete cds. ACCESSION M59852 KEYWORDS beta-D-glucosidase. SOURCE A.tumefaciens DNA. ORGANISM Agrobacterium tumefaciens Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Rhizobiaceae. REFERENCE 1 (bases 1 to 3710) AUTHORS Castle,L.A. and Morris,R.O. JOURNAL Unpublished (1990) STANDARD full staff_entry FEATURES Location/Qualifiers RBS 169..173 /gene="cbg1" CDS 181..2637 /EC_number="3.2.1.21" /product="beta-D-glucosidase" /gene="cbg1" /codon_start=181 misc_feature 213..223 /note="active site" /gene="cbg1" BASE COUNT 718 a 1084 c 1168 g 740 t ORIGIN 1 cgatcggctc gggacacgga cagaattact cactgctcta tgccttggcg gcggtcagtg 61 cctttttggg cgcgctggca atcgtgccgg tacgtggcgc acgttgataa gatcaatatt 121 tcgggcggga acgcctgcct aatggtcccc atcggaccga ttaacatggg aggatcccct 181 atgatcgacg atattctcga taagatgaca ctggaggagc aggtgtcgct gctctcgggc 241 gcggatttct ggacgaccgt cgcgatcgag cggctcggcg tgccgaagat caaggttacc 301 gacggcccca atggcgcacg cggcggcggg tcgctggtcg gcggcgtcaa gtccgcctgc 361 ttcccggtgg caatcgcgct tggagcgacg tgggacccgg agctcatcga gcgcgccggc 421 gtggcgctgg gaggacaagc caagagcaag ggcgcgtcgg tgcttctggc gccgaccgtc 481 aacattcacc gctccggcct caatggccgc aacttcgaat gctattcgga agacccggcg 541 ctgaccgccg cctgcgccgt cgcctatatc aatggcgtgc agagccaggg tgtggccgcc 601 acgatcaagc acttcgtcgc caacgagtcc gagatcgagc ggcagaccat gtcctccgat 661 gtcgatgagc ggacgctgcg cgaaatctat ctgccgcctt tcgaggaggc ggtgaagaag 721 gccggcgtga aggccgtcat gtcctcctac aacaagctca acggcaccta tacgagcgaa 781 aatccctggc tgctgacgaa agtcctgcgc gaggaatggg gcttcgacgg cgtggtcatg 841 tccgactggt tcggctcgca ctcgacggct gaaaccatca atgccgggct cgatctggag 901 atgccggggc cttggcggga tcgcggcgaa aagctggtcg ccgcagtccg ggaaggcaag 961 gtaaaggccg agaccgtgcg cgcttcggca cggcgtattc tccttctgct cgaacgcgtc 1021 ggcgcctttg aaaaggcgcc tgatctcgcc gaacacgcgc ttgatctgcc ggaagatcgt 1081 gcgctcatcc gccaactcgg tgcggagggt gctgtactcc tgaagaatga cggagtgctg 1141 ccgcttgcca agtcgtcctt cgaccagatc gccgtcatcg gccccaatgc ggcttccgca 1201 cgcgtcatgg gcggaggaag cgcgcggatt gccgcgcatt atacggtgag cccacttgag 1261 ggcattcgcg cggcgctgtc caacgccaac agcctccgcc atgcggtcgg ctgtaacaac 1321 aaccggctca tcgacgtctt cagcggcgag atgacggtgg aatacttcaa gggacgcggc 1381 ttcgagagcc gtccggtcca tgtcgagacc gtcgaaaagg gcgaattctt ctggttcgat 1441 cttccgtccg gcgaccttga tctcgccgat ttttcggcgc gcatgacggc gaccttcgtg 1501 ccgcaggaaa ccggtgaaca catcttcggc atgaccaatg ctgggcttgc tcggctgttc 1561 gtggacggcg aactggtggt cgatggctat gacggttgga cgaagggtga gaactttttt 1621 ggaaccgcga acagcgagca gcgtcgggcg gtaacgcttg gggccgcacg ccgctaccgg 1681 gttgtggtcg aatatgaggc gccgaaggcc agcctggacg gcatcaacat atgtgcgctc 1741 cgcttcggtg tcgaaaagcc gctcggcgat gccgggattg cggaggcggt cgaaaccgcc 1801 cgcaagtccg atatcgtact gctcctcgtc ggccgtgagg gcgagtggga caccgaaggt 1861 ctggatctgc ccgacatgcg cctgccgggt cgccaggagg agctgatcga ggcggtcgcc 1921 gaaaccaatc ccaacgtggt cgtggtactg caaacgggtg gtcccatcga gatgccatgg 1981 ctcggcaagg tgcgtgcggt gctgcagatg tggtatcccg gccaggaact tggcaatgcg 2041 cttgcggacg ttctctttgg tgatgtcgag cctgccggcc gcttgccaca gaccttcccg 2101 aaggcgctca cggataattc cgccattacc gacgatccgt cgatctatcc tggccaggac 2161 ggccatgtgc gctacgcgga agggatcttc gtcggctatc gccatcacga tacaagagag 2221 atcgaaccac tcttcccctt cggcttcggt cttggctaca cccgctttac ctggggtgcc 2281 ccgcaactat cgggaacgga aatgggggcg gatggtctta cggtgacggt cgatgtcacc 2341 aatataggcg acagggcggg atcggacgtg gtgcagctct atgttcactc tcccaatgcc 2401 agggtcgagc ggccgttcaa ggagctgcgt gcctttgcga agctcaagct ggccccgggc 2461 gcgaccggta cggcggtgct gaagatcgct cctcgcgact tggcttactt cgatgtcgag 2521 gccggtcgtt tccgggctga tgcgggcaag tacgagctga tcgtggcggc cagcgccatc 2581 gatatccggg caagcgtaag tattcacttg ccggtcgatc atgtgatgga gccgtagcgc 2641 aagcattcaa tatgagccgc gccgcctatc ttagaaggag gcggcgcgat ccgcgaagtg 2701 agtgggacag ggcctggcga tcgctctctt aatgtacata cctgggatgt atatttccag 2761 gccgatgcgc tatgatacat ccatgtacac acatgggagc atcccgctat gcagatgacc 2821 aaggcattca agaacggcaa ttcccaagcc gtacgcatcc cagcagagat tgcctatgag 2881 cgcacggaca tggagctgga gattgagcgg gtaggcgatg aaatccgcat tcgtcctgtc 2941 cgccagtcgt tggccggcgt catggatgtt ttcgcacgct tttcccccaa tttcatgagc 3001 gagggcaggg gtgaccaaga gcaggacgag cgcgagagaa gatctgatgc cgcgctatat 3061 gctcgatacg aatatgtgca tctatctgat gaagaaccag cccgagcaag tcgctcgtcg 3121 ttttgccgct tgctacgtcg gtgacgtggt gatgtcggcc attaccttcg cggagctgga 3181 ttacggtgtt gccgcgtctg atgccccgga acgggagcgc ggcaaccttg atgccttggt 3241 gaccctcatt gcgtagagcc gttcgatatc gccgctgccc acgcttatgg cccagtacgc 3301 atggcaacgc gagagcaaga aagaccatct gacagctgat cgcatcgcgc tcattgcctt 3361 gatacgtgct gtaccacaac acacgagatt catgctatcc actcaatcga actgctgata 3421 ctgaggctgc gttcgtgctt acggcagcgc cgactaaagg aaccatcgtc ccttagtcat 3481 ctcagatcca tgccttcatc ggcattgcga tcatgacaca cggctcattt cagacttgga 3541 aacagcctag aacaaccgct ccagccagcc gtgcttgtcg ggcgcacgtc cgttttgaat 3601 gtcgaggagt gccgccttca ggcgcgtggt caccggaccg gcgccgccgt cgccaatggt 3661 gaaattgtgc ttgcggccct tgagcttgcc gatcggagtc accactgcag //