GenBank-Updates@genbank.bio.net (03/06/91)
LOCUS PSENAHGH 2160 bp ds-DNA BCT 06-MAR-1991
DEFINITION Pseudomonas putida salicylate hydroxylase (nahG) gene, complete
cds, and nahH gene, 3' end.
ACCESSION M60055 J05317
KEYWORDS salicylate hydroxylase.
SOURCE P.putida (strain PpG7) DNA.
ORGANISM Pseudomonas putida
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Pseudomonadaceae.
REFERENCE 1 (bases 1 to 2160)
AUTHORS You,I.-S., Ghosal,D. and Gunsalus,I.C.
TITLE Nucleotide sequence analysis of the Pseudomonas putida PpG7
salicylate hydroxylase gene (nahG) and its 3'-flanking region
JOURNAL Biochemistry 30, 1635-1641 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS 1..1305
/EC_number="1.14.13.1"
/product="salicylate hydroxylase"
/gene="nahG"
/codon_start=1
RBS 1792..1796
/note="ORF"
CDS 1802..2128
/note="ORF"
/codon_start=1802
repeat_unit 1954..1962
/rpt_type=inverted repeat
repeat_unit 1991..1998
/rpt_type=inverted repeat
RBS 2123..2131
/gene="nahH"
CDS 2137..2160
/gene="nahH"
/codon_start=2137
/partial
BASE COUNT 404 a 634 c 698 g 424 t
ORIGIN
1 atgaaaaaca ataaacttgg cttgcgcatc ggtatcgtcg gcggcggaat ttccggcgtt
61 gccttagcac tggaactctg ccgctactcc catatccagg tacagctgtt cgaggctgcg
121 ccggctttcg gtgaggtcgg tgccggcgtg tcctttggcc ccaacgcggt gcgcgccatt
181 gtcggcctgg gcttgggcga ggcctacctg caggttgccg accgtacttc ggagccctgg
241 gaggacgtgt ggttcgaatg gcggcgcggc agcgatgcca gctatctggg agccaccatc
301 gctccgggcg tgggccagtc ctcggtacac cgggcggatt tcatcgacgc cctagtaact
361 cacctcccag aaggtatcgc ccaattcggg aagcgcgcca cccaggtcga gcagcagggt
421 ggcgaagtgc aagtgctgtt caccgacggc acagagtacc gctgcgacct tctgatcggt
481 gccgacggaa tcaagtcagc gctccgtagc catgtgctgg aaggtcaggg gctggcccca
541 caagtgccgc gattcagcgg cacctgtgcc tatcggggga tggtcgacag cctgcatctg
601 cgagaagcct atcgggccca tggcatcgac gagcacttgg tggacgtgcc gcagatgtac
661 ctagggctcg acggccatat cctcaccttt ccagtgagga atggcggcat catcaacgtg
721 gtggctttca tctccgaccg tagcgagccg aagccgacct ggcctgcgga tgccccttgg
781 gtgcgtgagg cgagccagcg cgagatgctc gatgccttcg cgggttgggg ggatgccgcg
841 cgcgccctgc tggagtgcat cccggcacca actctctggg cactgcatga cctggcggag
901 ctgccgggct acgtgcacgg tcgggtcgtc ctgatcggcg acgcagctca cgccatgctg
961 ccgcaccaag gtgccggtgc tggccaaggg cttgaggacg cctacttcct cgcccgcctg
1021 ttgggcgata cgcaggccga tgccggcaac ctcgccgagc tgcttgaagc ctacgacgac
1081 ctgcgccgcc ctcgtgcctg tcgcgtgcag caaacctcct gggagaccgg cgagttatac
1141 gagttgcgcg accccgtcgt cggtgcgaac gagcagctgc tgggggaaaa cctggcgacc
1201 cgcttcgact ggctgtggaa ccacgacctc gacactgacc tggccgaggc ccgtgcgcgg
1261 ctgggttggg agcatggtgg cgggggtgcg ctacgtcaag ggtgacaacg ttgacaccga
1321 tcgcggattc gacggcaaat agttcgagtg cgttctggac atcggctatg tggtgcagag
1381 gggctcgctt aaggggctga gcgtcagggg tggaatgtca ctgcgagttc catccaccag
1441 actgacgtcg ataagaaccg cctgatcgtt aactacacgt gaaagctgat ctaagcatgg
1501 gcggcctacc gaccctagcg tcagtgggcc gcagccacaa cggcaaccta atatggtttc
1561 ccttgcatgc tgcgccgcag agcgtcgctg gagataacaa tgcacaacaa taacaagatg
1621 ctttgctcgt tccgttccac ctttaccgtc agtgctctgg cattggccct ctgcaactcc
1681 ctggcaagag cggtcaaggc ggccgaaccg atcaagattg gtcttttgat ttccgacagc
1741 ggcatcttcg cggccccgag tgaagctact ccgtttctgc cacggttgag cggagggaga
1801 tatgtcagag gtctttgaaa tcactgtgca gcctggtgga gagcgctttg tctgtcagcc
1861 tcagcaatca gcgttgcatg ccatggagac gcagggcaag cgctgcttac ctgtgggctg
1921 tcgcggcggc ggttgtggct tgtgcaaggt gagggtgctt gccggtgact acgagagcgg
1981 gcgcgtgagc tgcaagcacc taccggtaga agcacgcgaa caaggctatg ccttggcctg
2041 ccgactgttt gcccgcagcg atctttgtat cgagcgttac tcaaagccgt gcagtgaaag
2101 tacggtcgac caacaacaaa gagaataggt gatttcatga acaaaggtgt aatgcgcccc
//