GenBank-Updates@genbank.bio.net (03/06/91)
LOCUS TRN4654RE 831 bp ds-DNA BCT 06-MAR-1991
DEFINITION Transposon Tn4654 resolvase (tnpR) gene, complete cds, and
transposase (tnpA) gene, 3' end.
ACCESSION D90148
KEYWORDS class II transposon; resolvase; tnpA gene; tnpR gene; toluene
degradative transposon; transposon.
SOURCE Pseudomonas putida (strain mt-2) transposon Tn4653 on TOL plasmid
pWWO DNA.
ORGANISM Transposon Tn4654
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 831)
AUTHORS Tsuda,M., Minegishi,K.-I. and Iino,T.
TITLE Toluene transposons Tn4651 and Tn4653 are class II transposons
JOURNAL J. Bacteriol. 171, 1386-1393 (1989)
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Masataka Tsuda
Department of Microbiology
Yamaguchi University School of Medicine
Ube, Yamaguchi 755
Japan
Phone: 0836-22-2227
Fax: 0836-22-2315
FEATURES Location/Qualifiers
misc_binding 52..93
/note="put. resolvase binding site II"
misc_signal 84..89
/note="put. -35 region"
misc_binding 98..127
/note="put. resolvase binding site III"
misc_signal 107..112
/note="put. -10 region"
misc_binding 180..186
/note="put. SD sequence"
CDS 190..750
/note="resolvase (gtg start codon, tnpR)"
CDS 754..>831
/note="transposase (tnpA)"
BASE COUNT 181 a 254 c 254 g 142 t
ORIGIN 1 bp upstream of SalI site.
1 gtcgactttg gatatttcgt gctgtcgcct tctgaaagtg acagccgcgc cgcttagtca
61 gaatagagtc atctttcgca tttttgacac atgcctgcga aggtcataga tttcagcctg
121 acagaaacgg ggtttgaggc acaacggaac agaaggagca cttaagccgc cttcaaccaa
181 ggagacatcg tgcaggggca ccgcatcggc tacgtccggg tcagcagctt cgaccagaac
241 ccggaacgcc agctggaaca gacacaggtg agcaaggtgt tcaccgacaa ggcatcgggc
301 aaggacaccc agcgccccca gctcgaagcg ctgctgagct tcgtccgcga aggcgataca
361 gtggtagtgc acagcatgga tcgcctggcg cgcaacctgg atgacctgcg ccgccttgtg
421 cagaagctga cccagcgcgg cgtgcgtatc gaattcctga aagagggcct agtcttcacc
481 ggcgaggact cgccgatggc caacctcatg ctgtcggtga tgggtgcctt cgccgagttc
541 gagcgcgccc tgatccgcga gcggcagcgt gagggtatcg ccttggccaa gcagcgtggc
601 gcgtaccgag gccgcaagaa agccctgtca gatgagcagg ctgctaccct gcggcagcga
661 gcgacggccg gcgagcccaa ggcgcagctt gcccgcgagt tcaacatcag ccgggaaacc
721 ctctaccagt acctccgcac ggacgactga tacatgccgc gtcgcttgat cctctcggcc
781 acggagcggg acaccctgct tgcgttgcca gaaagccagg atgacatgat c
//