GenBank-Updates@genbank.bio.net (03/06/91)
LOCUS STYLIVBC 3617 bp ds-DNA BCT 06-MAR-1991
DEFINITION S.typhimurium livB and livC gene.
ACCESSION D00478
KEYWORDS leucine-,isoleucine-,valine-binding protein; livB; livC.
SOURCE Salmonella typhimurium (strain LT2) genomic DNA.
ORGANISM Salmonella typhimurium
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 3617)
AUTHORS Ohnishi,K., Nakazima,A., Matsubara,K. and Kiritani,K.
TITLE Cloning and nucleotide sequence of livB and livC, the structural
genes encoding proteins of the high-affinity branched-chain amino
acid transport in Salmonella typhimurium
JOURNAL J. Biochem. 107, 202-208 (1990)
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Kazuyoshi Kiritani
Department of Microbiology
School of Pharmacy
Hokuriku University
Ho-3 Kanagawa-Machi, Kanazawa 920-11
Japan
Phone: 0762-29-1161
FEATURES Location/Qualifiers
misc_signal 194..223
/note="promoter region for livB"
CDS 355..1452
/note="livB protein precursor"
sig_peptide 355..417
/note="livB signal peptide"
mat_peptide 418..1452
/note="livB protein"
misc_signal 1499..1531
/note="terminator"
misc_signal 2252..2282
/note="promoter region for livC"
CDS 2452..3561
/note="livC protein precursor"
sig_peptide 2452..2520
/note="livC signal peptide"
mat_peptide 2521..3561
/note="livC protein"
misc_signal 3583..3603
/note="attenuator"
BASE COUNT 934 a 898 c 971 g 814 t
ORIGIN Map position 76 min.
1 aagcttcgcg ctgcgatcga agcgtaatct tcgcgaattg ccaatgaacc ctcgaatgtg
61 aattcggggg ttttgttttt tgtaggctgg ataaggcgtt tacgctgcca tccggcaata
121 cgtttgcccg atggcgcttc gcttatcggg cctacggacc cttcctctcc cccaccgaca
181 caaatctgtt atctggatgt aaatttagct attccaaaat aataaaaatc gggtatgttt
241 tagcagagta tgctgctaaa gcacgggtag ctagccaata atcgaaataa agtgctgaac
301 aataacacca caacacacgt aacaaccaga ataatgggga ttatcaggat gaatatgaag
361 ggtaaaacgt tattggcagg atgtatcgcc ctgtcattaa gccatatggc attcgccgac
421 gacattaaag tcgcggttgt cggcgcgatg tccggtccgg tggcgcagta tggcgaccag
481 gaatttaccg gtgcggagca ggcaattgcc gatatcaatg cgaaaggcgg tattaaaggc
541 gataagctcg tcgcggtgaa atatgacgac gcctgcgacc cgaaacaggc ggtagctgtc
601 gctaacaaag tggtgaacga cggcatcaag tatgttatcg gtcacttatg ttcctcttcc
661 acacagccag cgtctgatat ctacgaagac gaaggcatcc tgatgataac cccggcggcg
721 accgcgccgg agctgaccgc gcgcggctat aagttggttt tgcgcaccac cggtctggac
781 tctgaccagg ggccaacggc ggcaaagtat attctggaga aggtaaaacc gcagcgcatc
841 gcgattatcc acgataagca gcagtacggc gaagggctgg cgcgcgcggt gcaggacggt
901 ctgaagaaag gcggcgttaa cgtcgtattc tttgacggca tcaccgctgg cgaaaaagat
961 ttctccactc tggttgcgcg cctgaagaaa gagaatatcg actttgtcta ctacggcggt
1021 tatcacccgg aaatggggca gattttgcgc cagtcccgcg ccgcagggct gaaaacccag
1081 tttatggggc cggaaggggt ggcgaacgtg tcgctgtcta acatcgccgg agagtcagcg
1141 gaaggcttac tggtcaccaa accgaagaac tacgaccagg tcccggcgaa caaaccgatt
1201 gtggatgcca tcaaagccaa gaaacaagat cctagcggcg cattcgtgtg gaccacctac
1261 gccgcgctgc aatcgttgca ggcgggcctg aaccactccg acgatccggc ggaaatcgcc
1321 aaatacctga aaggcgccac ggtcgacacc gtaatgggac cgctgtcgtg ggatgagaaa
1381 ggcgatctga aaggatttga gttcggcgtg tttgactggc atgcgaatgg tacggcgaca
1441 gacgctaagt gattttctgg cgcctttcgc gctgcaggtg cgttggctac gttattcaac
1501 cccggtcact tacttatgta agctcccggg gatttcataa cttgccgcct gcctgcaacg
1561 cgaaattctt ggaaaattgc gaggtggttt ttatcttttc tcccggcatg taggccggat
1621 aagcgtcagc gccatccggc tttcttttag cgcttctccc agccgtcatg ctgggcggta
1681 aaacctaacg cctgcataaa cgcggccatc acgctgcggt cttcaacacc gacatccgcc
1741 atccaccacg acgagacgtt gggattatcg cggatgactt cttctaccag gtattgcccg
1801 acgccgcggc gacggctgat ttcacgcacg cgtaaggaat ccagcgcgcc ctgcgttccg
1861 ctcagcgtca ctcttacggc gcccaacaaa cgctcattaa accgcgcggc ataaatccgg
1921 tgcgtttcat ctacgcttaa tgaggaggcg gaatattccg gccagatttt gcccagatcg
1981 atctgatcct gagcgctaaa gtgttctaaa cgaagaatgg tcatcttcag cgacggcttg
2041 tcccgatttc aaaagatgcg atcagtgtac cgaaacaggc aggccagatg ctttctcttt
2101 tttgtgtcat ttaccaggtt attaattttt taacaacgat aattacagtt tgaagcatta
2161 gagaatggaa aacaggcagc ataattccct gaatgctagt gaattattcc gcttaatatg
2221 ccgttatttt atgctgacaa gcatgctttt ttttgtttat ctatagcgaa aagcagaata
2281 ttatcttttc ttaatagact gaaaaataga gattttaatc ttattatgct ttaaatcgtg
2341 cgctaactca ttaatgagtc agtaaaaagc gcaccattta taaaaagtac agtctgcttt
2401 ttaaccagca aaaaacaaaa catataacat cacgaatggg gatacaggca catgaaacgg
2461 aaagcgaaaa caataatcgc agggattgtt gcattagcag tctcgcaggg ggcaatggca
2521 gatgatatta aagtcgccat agtcggggcg atgtccggcc cggtagcgca atggggcgat
2581 atggaattta acggcgcgcg ccaggccatt aaagacatca acgcgaaagg cgggattaaa
2641 ggcgataagc tggtcggcgt agagtacgat gatgcctgcg atccaaaaca ggcggtggcg
2701 gtggccaaca aaatcgttaa cgacggtatt cagtacgtta ttggtcactt gtgttcttct
2761 tctactcagc cagcatccga tatctatgaa gatgaaggta ttctgatgat ctccccgggg
2821 gcgactaacc cggagctgac ccagcgcggc tatcagtaca ttatgcgtac cgccggcctg
2881 gactcctccc aggggccaac agccgcgaaa tacatcctgg aaacggtgaa accgcagcgc
2941 atcgctatca ttcacgataa acagcaatac ggcgaaggac tggcgcgctc cgtgcaggat
3001 ggcctgaagc agggcaatgc caatattgtc ttttttgatg gtattaccgc tggcgaaaaa
3061 gatttctccg ccctgattgc ccgcttgcaa aaagagaata tcgactttgt gtattacggc
3121 ggctactacc cggaaatggg gcagatcgta cgccaggcgc gggctaatgg cctgaaaacg
3181 caatttatgg ggccggaagg cgtaggtaac gcgtcgctgt ccaatattgc gggcggtgcg
3241 gcggaaggca tgttggtgac gatgccaaaa cgttatgacc aggacccggc gaataaagcg
3301 attgtcgaag cgctgaaagc cgacaagaaa gatcccagcg gtccgtacgt ctggatcacc
3361 tacgccgccg tccagtcact ggcgaccgca atgacgcgta gcgccagcca tcgtccgctg
3421 gatctggtga aagatcttaa agctaacggg gctgataccg ttattgggcc gctgaaatgg
3481 gatgaaaaag gcgatcttaa gggatttgaa tttggcgtct tccagtggca cgccgacggc
3541 tcgtcaaccg tcgcgaagta atgccagaag ataagttatc ccaccgcccg cacgccgggc
3601 ggtttaaaaa ggttacc
//