GenBank-Updates@genbank.bio.net (03/06/91)
LOCUS TTHLDHYT 1133 bp ds-DNA BCT 06-MAR-1991
DEFINITION T.aquaticus gene for L-lactate dehydrogenase, complete cds.
ACCESSION D00585
KEYWORDS L-lactate dehydrogenase; oxidoreductase.
SOURCE Thermus aquaticus (strain YT-1) DNA.
ORGANISM Thermus aquaticus
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Neisseriaceae.
REFERENCE 1 (bases 1 to 1133)
AUTHORS Ono,M., Matsuzawa,H. and Ohta,T.
TITLE Nucleotide sequence and characteristics of the gene for L-lactate
dehydrogenase of Thermus aquaticus YT-1 and the deduced amino acid
sequence of the enzyme
JOURNAL J. Biochem. 107, 21-26 (1990)
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Makoto Ono
Department of Agricultural Chemistry
The University of Tokyo
Bunkyo-ku
Tokyo 113
Japan
Phone: 03-812-2111 x5151
Fax: 03-812-0544
The nucleotide sequence had high homology (85.8% identity) with the
LDH gene of T. caldophilus.
FEATURES Location/Qualifiers
CDS 166..1098
/note="L-lactate dehydrogenase (EC 1.1.1.27) (gtg start
codon)"
BASE COUNT 161 a 372 c 414 g 186 t
ORIGIN 2 bp upstream of RsaI site.
1 gtactccacg taggcccctt cggggacctc cagggtgagg gggcccttca ggggtatggg
61 gttcctttcc cagtcggtga agtccccgat cagggccttg gcctggggcg gagggtagaa
121 ggtgacgcgc cttttgccga tctccaccac gggtatactc tacccgtgaa ggtcggcatc
181 gtgggtagcg gcttcgtggg gagcgccacc gcttacgccc tggtcctcca gggggtggcc
241 cgggaggtgg tgctggtgga cttggatcgg aagctggccc aggcccacgc cgaggacatc
301 ctccacgcca ccccctttgc ccacccggtc tgggtgcgct ccggctggta cgaggacctg
361 gagggggcca gggtggtgat cgtggccgcg ggggtggccc aacgcccggg ggagaccagg
421 ctccagcttc tggaccgcaa cgcccaggtc ttcgccgacg tggtccccaa gatcctgaag
481 gccgccccgg aggcggtcct cctgatcgcc acgaatcccg tggacgtgat gacccaggtg
541 gcctaccgcc tctccggcct acccccggag cgggtggtgg gctcggggac catcctggac
601 accgcccgct tccgggccct cctggcccag cacctcctgg tggcgcccca gtcggtccac
661 gcctacgtgg tgggggagca cggggactcg gaggtcctgg tttggtccag cgcccaggtg
721 ggaggggtag acctcgaggc cttcgcccag gccagggggc gcgcccttac ccccgacgac
781 cgcctccgga tagacgaagg ggtgcgccgg gccgcttacc gcatcattga gggcaagggg
841 gccacctact atggcatcgg agcgggcctg gcccggctca cccgggccat cctcaccgac
901 gagaaggggg tgtttacggt tagcctcttc accccggaag tggagggggt ggaggaggtg
961 gccctttccc tgccccgcat cctgggggct cgtggggtgg aggccaccct ttacccccgg
1021 ctgaacgagg aggagcgcca ggccctcagg cggagcgccg agatcctcaa aggggcggcc
1081 tcggccttgg gcttttaaac cttgtccaga tgggggaaga gggtgaggag ctc
//