GenBank-Updates@genbank.bio.net (03/06/91)
LOCUS WMVCP 3332 bp ss-RNA VRL 06-MAR-1991
DEFINITION Watermelon mosaic virus 2 49kda protease, nuclear inclusion protein
and coat protein genes.
ACCESSION D00592
KEYWORDS 49kda protease; coat protein; nuclear inclusion protein.
SOURCE Watermelon mosaic virus 2, cDNA to viral RNA, clone pWMVII-3.2.
pWMVII-3.2.
ORGANISM Watermelon mosaic virus 2
Viridae; ss-RNA nonenveloped viruses; Rod-shaped ss-RNA viruses;
Potyvirus.
REFERENCE 1 (bases 1 to 3332)
AUTHORS Quemada,H., Sieu,L.C., Siemieniak,D.R., Gonsalves,D. and
Slightom,J.L.
TITLE Watermelon mosaic virus II and zucchini yellow mosaic virus:
Cloning of 3'-terminal regions, nucleotide sequence, and phylogenic
comparisons
JOURNAL J. Gen. Virol. (1990) In press
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Jerry L. slightom
The Upjohn Company
Molecular Biology Unit 7242
Kalamazoo, MI 49007
U.S.A
Phone: 616-385-5304
Fax: 616-385-7373
The longest ORF contains a translation termination codon at
position 803. They suggest the presence of this termination codon
is most likely an artifact of the recombinant DNA process in [J.
Gen. Virol. (1990) In press].
FEATURES Location/Qualifiers
mat_peptide <1..658
/note="49kda protease"
mat_peptide 659..2209
/note="nuclear inclusion protein"
misc_feature 803..805
/note="termination codon in ORF"
mat_peptide 2210..3052
/note="coat protein"
misc_feature 3309..3332
/note="poly(A) stretch"
BASE COUNT 1043 a 550 c 813 g 926 t
ORIGIN 1016 bp upstream of BamHI site.
1 tctgtcggtc acaaggaaac aatgtttgga gttgggtatg gttctttcat tatcaccaat
61 gggcacttat ttagaaggaa caatgggatg cttacagtta aaacatggcc atggcggatt
121 tgtcatacat aacaccacgc agttaagaat ccattttatt caaggtaaag atgcaatctt
181 gattcgtatg ccgaagattt tcctccgttt gcaaagcgca acttctttag acaaccaaaa
241 cgtgaggaac gagtttgcat ggttggaaca aactttcaag agaagagctt acgggcaaca
301 gtctcagagt tcttccatta ttctacctga aggaaaggga tctttctgga tacattggat
361 aacgactcag gatggttttt gtgggttacc tcttgtgtct gtcaatgacg gacatgttgt
421 tggtatacat ggattaacat ctaatgattc agagaagaat ttctttgttc catttaccga
481 tgggttcgag aaggagtatt tggacaacgc tgataatcta tcgtgggaca aacattggtt
541 ttgggaaccg agtaagattg cgtggggccc tttgaactta gtagaggagc agccaaagga
601 agagtttaag atatcaaaac ttgtatctga tctctttggt aacacagtag cagtgcagag
661 cagaaaggaa agatgggttc tggatgccat ggaagggaat ttagtggctt gtgggcaagc
721 tgatagcgca ctagtgacaa agcatgtggt taaaggaaag tgcccttatt ttgcgcaata
781 tctctcactg catgatggag cgtgacagtt ctttgagcca ttgatggggg cataccagcc
841 aagccggttg aataaagacg ctttcaagaa agatttcttt aaatacaaca aaccggttgt
901 tctgaatgaa gttgacttta atgcattcga gaaagcagtt gagggagtga taacaatgat
961 ggttgacttt gaattcgcag aatgcttatt tgtgactgat cctgacgaaa tttatggatc
1021 cctgaacatg aaagctgctg ttggtgctca atataaagga aagaaacagg actacttttc
1081 tggcatggat agttttgata aagagcgctt gttgtacctt agctgtgaga gactttttaa
1141 tggagagaag ggaatttgga atggttcctt aaaggcggag ttgagaccaa ttgagaaagt
1201 gcaagcaaac aaaacaagaa cattcacagc agcaccgctt gacacattac ttggagctaa
1261 agtttgcgtt gatgatttca ataatcaatt ttatagcttc aatttaaaat gtccgtggac
1321 agttggcatg actaagtttt atgggggttg ggataagcta atgagaagtt tgcctgatgg
1381 ctggacatat tgccacgcag atgggtcaca gttcgacagt tctctaactc cattgttgtt
1441 gaatgctgtc ctcagtatca gatgttgctt catggaagat tggtgggtcg gaagggaaat
1501 gcttgaaaac ctttacgctg agatagttta cacaccaatc ttagcacctg atggtacaat
1561 ttttaaaaag ttcagaggga acaacagtgg gcagccatct acagttgttg acaatacact
1621 tatggttgtc attgctatgt attattcgtg ctgtaaacaa ggctggtcag aggaggacat
1681 tgaaaggagg ctggtgttct ttgccaatgg tgatgatatt atcctggcag ttaaggatga
1741 agatgtgtgg ctgtatgaca ctctaagtgc ttcattcgct gagctgggcc taaattacaa
1801 ttttgatgag cgaacaaaga aaagagagga actgtggttc atgtcacacc aggccatgct
1861 agttgatgga atatacattc caaagcttga acctgaaagg atagtttcca tcttagagtg
1921 ggataggagc aaggaactta tgcaccgcac tgaggcaatt tgtgcagcta tgatagaagc
1981 atggggatat actgagcttt tgcaggaaat tcgcaaattc tatttgtggc ttctgagcaa
2041 agatgaattt aaggaacttg ctgcatccgg aaaagctcca tacatagctg agacagcatt
2101 gagaaaactc tacacagatg tcaacacaca accaagtgaa ttgcaaagat accttgaagt
2161 tttggatttt aaccatatcg atggttgctg tgaatcagtg tctttacaat caggaaaaga
2221 aacagttgaa aatttggacg cagggaaaga atcaaagaaa gatgccagtg acaaagggaa
2281 taagccgcag aactcgcaag ttggtcaggg tagcaaggaa ccaacaaaaa ccggcacagt
2341 cagcaaggat gtaaatgttg gatcgaaagg aaaagaagtc ccacgactac aaaagataac
2401 aaagaaaatg aatcttccaa cagttggtgg gaaaatcatt cttagcttag accatttgct
2461 tgagtacaaa cctagtcaag ttgatttgtt taacactcga gcaacaaaaa cacaatttga
2521 atcatggtac agcgcagtca aagttgagta tgatcttaat gatgagcaaa tgggtgtgat
2581 tatgaatggt tttatggttt ggtgtatcga taacggtaca tctccagatg tcaatggagt
2641 gtgggtaatg atggatgggg aagagcaagt tgagtaccca ctaaagccaa ttgttgaaaa
2701 tgcaaagcca actttgagac aaatcatgca ccatttctca gatgcagcgg aagcatatat
2761 tgaaatgaga aactctgaaa gtccgtatat gcctagatac ggattactga gaaatttgag
2821 agacagggaa ttagcacgct atgcttttga cttctatgag gttacttcta aaacgccaaa
2881 tagggcaaga gaagcaatag ctcaaatgaa ggccgcggct ctcgcgggag ttaacagcag
2941 gttatttgga cttgatggta atatctcgac caattccgaa aatactggga ggcacactgc
3001 aagggacgta aatcagaata tgcatacttt gttgggtatg ggtccaccgc agtaaagact
3061 aggtaaactg gtcacagtta gcatttcggg tcgttataag ttttctataa tataacatgt
3121 cgcactttat tttagtatag tgtgatttca tcacctttgt actgtttatg ttagcgtggt
3181 ttaaccacct ttgtgtgtgc tttatattat agtttatgcg tagcagggag aaccattaca
3241 atgccggagt tgtttgtagt gtgatttcat cacggttaat agccgaggta cggtaatgtt
3301 tgttgcctaa aaaaaaaaaa aaaaaaaaaa aa
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