GenBank-Updates@genbank.bio.net (03/06/91)
LOCUS GCALIP2 1767 bp ss-mRNA BCT 06-MAR-1991
DEFINITION G.candidum mRNA for lipase II.
ACCESSION D00697
KEYWORDS lipase; lipase II.
SOURCE G.candidum, cDNA to mRNA, clone pGCL2.
ORGANISM Geotrichum candidum
Eukaryota; Plantae; Thallobionta; Basidiomycotina;
Deuteromycotina.
REFERENCE 1 (bases 1 to 1767)
AUTHORS Shimada,Y., Sugihara,A., Iizumi,T. and Tominaga,Y.
TITLE cDNA cloning and characterization of Geotrichum candidum lipase II
JOURNAL J. Biochem. 107, 703-707 (1990)
STANDARD full staff_review
COMMENT The N- and C-terminal amino acid of the mature lipase II were
determined.
FEATURES Location/Qualifiers
CDS <1..1674
/note="lipase II precursor"
sig_peptide <1..39
/note="lipase II signal peptide"
mat_peptide 40..1671
/note="lipase II"
misc_signal 682..696
/note="common sequence of lipase (Gly-X-Ser-X-Gly)"
misc_feature 886..894
/note="potential N-glycosylation site"
misc_feature 1129..1137
/note="potential N-glycosylation site"
misc_feature 1767..1767
/note="polyadenylation site"
BASE COUNT 378 a 492 c 397 g 500 t
ORIGIN 178 bp upstream of BamHI site.
1 ttcttagccg ctgccgtaaa cctagctggc gtcctggccc aggccccacg gccgtctctc
61 aatggcaacg aggtcatctc tggtgtcctt gagggcaagg ttgatacctt caagggaatc
121 ccatttgctg accctccttt gaatgacttg cgattcaagc acccccagcc tttcactgga
181 tcctaccagg gtcttaaggc caatgatttt agccctgctt gtatgcagct tgatcctggc
241 aactctctca ctttgcttga caaagctctg ggattggcaa aagtcatccc cgaagaattt
301 agaggtcccc tttatgatat ggccaagggt accgtgtcga tgaatgagga ctgtctttac
361 ctcaatgttt tccgccctgc tggcaccaag cctgatgcta agctccccgt catggtttgg
421 atttacggtg gtgcgtttgt ttacggttct tctgctgcct accctggtaa cagctacgtt
481 aaggaaagta tcaacatggg ccagcccgtt gtgtttgttt ccatcaacta ccgtaccggt
541 ccatttggat tcctgggtgg tgatgccatc accgctgagg gcaacaccaa cgctggtctg
601 cacgaccagc gcaagggtct cgagtgggtt agcgacaaca ttgccaactt tggtggtgat
661 cccgataagg tcatgatttt cggtgagtcc gctggtgcca tgagtgttgc tcaccagctt
721 attgcctatg gtggtgacaa cacctacaac ggaaagaagc ttttccactc tgccattctt
781 cagtctggtg gccctcttcc ttaccacgac tctagctccg ttggtcccga tatttcctac
841 aacagatttg ctcagtatgc cggatgtgac actagtgcca gtgccaacga cactctggag
901 tgtctccgca gcaagtccag ctctgtcttg cacgatgccc agaactctta cgatctcaaa
961 gatctgtttg gtctactccc tcaattcctt ggatttggtc ccagacccga cggcaacatt
1021 attcccgatg ccgcttatga gctcttccgc agcggtagat acgccaaggt tccctacatt
1081 agcggtaacc aggaggatga aggtactgct tttgctcctg ttgctctcaa cgctaccacg
1141 actccccatg ttaagaagtg gttgcagtac attttctacg atgcttccga ggcttccatt
1201 gaccgtgttt tgtcgctgta cccgcagacc ctctctgttg gctcgccctt ccgcactggc
1261 attcttaatg ccctgacccc ccagttcaag cgtgttgcgg ccatcttgtc cgatatgctt
1321 ttccagtctc cccgccgcgt gatgcttagc gccaccaagg acgttaaccg ctggacttac
1381 ctttcgaccc atctgcacaa cctggtgcca tttttgggta ctttccatgg caacgagctt
1441 atcttccaat tcaatgtgaa cattggcccc gctaactcct accttcgtta ctttatttcc
1501 tttgccaacc accatgaccc taatgttggt actaacctgc tccagtggga tcaatacact
1561 gatgaaggca aggagatgct tgagattcac atgaccgata atgtcatgag aactgatgac
1621 tacagaattg agggaatctc aaactttgag actgacgtta atctctacgg ttaaaacctt
1681 ctgtttatag ccacttgcta tgcgccgtgc ataccgggga tgtaattttt aagtagctgt
1741 cctcaattaa aaaatttaaa gcattat
//