GenBank-Updates@genbank.bio.net (03/06/91)
LOCUS GCALIP2 1767 bp ss-mRNA BCT 06-MAR-1991 DEFINITION G.candidum mRNA for lipase II. ACCESSION D00697 KEYWORDS lipase; lipase II. SOURCE G.candidum, cDNA to mRNA, clone pGCL2. ORGANISM Geotrichum candidum Eukaryota; Plantae; Thallobionta; Basidiomycotina; Deuteromycotina. REFERENCE 1 (bases 1 to 1767) AUTHORS Shimada,Y., Sugihara,A., Iizumi,T. and Tominaga,Y. TITLE cDNA cloning and characterization of Geotrichum candidum lipase II JOURNAL J. Biochem. 107, 703-707 (1990) STANDARD full staff_review COMMENT The N- and C-terminal amino acid of the mature lipase II were determined. FEATURES Location/Qualifiers CDS <1..1674 /note="lipase II precursor" sig_peptide <1..39 /note="lipase II signal peptide" mat_peptide 40..1671 /note="lipase II" misc_signal 682..696 /note="common sequence of lipase (Gly-X-Ser-X-Gly)" misc_feature 886..894 /note="potential N-glycosylation site" misc_feature 1129..1137 /note="potential N-glycosylation site" misc_feature 1767..1767 /note="polyadenylation site" BASE COUNT 378 a 492 c 397 g 500 t ORIGIN 178 bp upstream of BamHI site. 1 ttcttagccg ctgccgtaaa cctagctggc gtcctggccc aggccccacg gccgtctctc 61 aatggcaacg aggtcatctc tggtgtcctt gagggcaagg ttgatacctt caagggaatc 121 ccatttgctg accctccttt gaatgacttg cgattcaagc acccccagcc tttcactgga 181 tcctaccagg gtcttaaggc caatgatttt agccctgctt gtatgcagct tgatcctggc 241 aactctctca ctttgcttga caaagctctg ggattggcaa aagtcatccc cgaagaattt 301 agaggtcccc tttatgatat ggccaagggt accgtgtcga tgaatgagga ctgtctttac 361 ctcaatgttt tccgccctgc tggcaccaag cctgatgcta agctccccgt catggtttgg 421 atttacggtg gtgcgtttgt ttacggttct tctgctgcct accctggtaa cagctacgtt 481 aaggaaagta tcaacatggg ccagcccgtt gtgtttgttt ccatcaacta ccgtaccggt 541 ccatttggat tcctgggtgg tgatgccatc accgctgagg gcaacaccaa cgctggtctg 601 cacgaccagc gcaagggtct cgagtgggtt agcgacaaca ttgccaactt tggtggtgat 661 cccgataagg tcatgatttt cggtgagtcc gctggtgcca tgagtgttgc tcaccagctt 721 attgcctatg gtggtgacaa cacctacaac ggaaagaagc ttttccactc tgccattctt 781 cagtctggtg gccctcttcc ttaccacgac tctagctccg ttggtcccga tatttcctac 841 aacagatttg ctcagtatgc cggatgtgac actagtgcca gtgccaacga cactctggag 901 tgtctccgca gcaagtccag ctctgtcttg cacgatgccc agaactctta cgatctcaaa 961 gatctgtttg gtctactccc tcaattcctt ggatttggtc ccagacccga cggcaacatt 1021 attcccgatg ccgcttatga gctcttccgc agcggtagat acgccaaggt tccctacatt 1081 agcggtaacc aggaggatga aggtactgct tttgctcctg ttgctctcaa cgctaccacg 1141 actccccatg ttaagaagtg gttgcagtac attttctacg atgcttccga ggcttccatt 1201 gaccgtgttt tgtcgctgta cccgcagacc ctctctgttg gctcgccctt ccgcactggc 1261 attcttaatg ccctgacccc ccagttcaag cgtgttgcgg ccatcttgtc cgatatgctt 1321 ttccagtctc cccgccgcgt gatgcttagc gccaccaagg acgttaaccg ctggacttac 1381 ctttcgaccc atctgcacaa cctggtgcca tttttgggta ctttccatgg caacgagctt 1441 atcttccaat tcaatgtgaa cattggcccc gctaactcct accttcgtta ctttatttcc 1501 tttgccaacc accatgaccc taatgttggt actaacctgc tccagtggga tcaatacact 1561 gatgaaggca aggagatgct tgagattcac atgaccgata atgtcatgag aactgatgac 1621 tacagaattg agggaatctc aaactttgag actgacgtta atctctacgg ttaaaacctt 1681 ctgtttatag ccacttgcta tgcgccgtgc ataccgggga tgtaattttt aagtagctgt 1741 cctcaattaa aaaatttaaa gcattat //