GenBank-Updates@genbank.bio.net (03/07/91)
LOCUS LISPRFA 1560 bp ds-DNA BCT 07-MAR-1991
DEFINITION L.monocytogenes listeriolysin regulatory protein (prfA) mRNA,
complete cds.
ACCESSION M55160
KEYWORDS lysteriolysin regulatory protein; prfA protein; transcriptional
activator protein.
SOURCE Listeria monocytogenes (strain EGD) DNA.
ORGANISM Listeria monocytogenes
Prokaryota; Bacteria; Firmicutes; Regular asporogenous rods;
Lactobacillaceae.
REFERENCE 1 (bases 1 to 1560)
AUTHORS Leimeister-Waechter,M., Haffner,C., Domann,E., Goebel,W. and
Chakraborty,T.
TITLE Identification of a gene that positively regulates expression of
listeriolysin, the major virulence factor of Listeria monocytogenes
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 87, 8336-8340 (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
-35_signal 262..266
/gene="prfA"
-10_signal 280..285
/gene="prfA"
RBS 399..404
/gene="prfA"
CDS 412..1119
/product="listeriolysin regulatory protein"
/gene="prfA"
/codon_start=412
terminator 1120..1170
/gene="prfA"
BASE COUNT 564 a 271 c 252 g 473 t
ORIGIN
1 gctgcagcat taaacaacaa agtagagcaa ttcgtactca acttaacatc ggaaaaagtt
61 cgaggattag gcatactaat catggacttc cccgaaaaac aaacaattaa aaacatcata
121 aaaaacaata aattcaacta acatatatta ttcctacaaa aaagggttag tatatctccg
181 agcaacctcg gaaccatata ctaactctat ttcaatttta acatctaaat aaatccgttt
241 ttaaatatgt atgcatttct tttgcgaaat caaaatttgt ataataaaat cctatatgta
301 aaaaacatca tttagcgtga ctttctttca acagctaaca attgttgtta ctgcctaatg
361 tttttagggt attttaaaaa agggcgataa aaaacgattg ggggatgaga catgaacgct
421 caagcagaag aattcaaaaa atatttagaa actaacggga taaaaccaaa acaatttcat
481 aaaaaagaac ttatttttaa ccaatgggat ccacaagaat attgtatttt tctatatgat
541 ggtatcacaa agctcacgag tattagcgag aacgggacca tcatgaattt acaatactac
601 aaaggggctt tcgttataat gtctggcttt attgatacag aaacatcggt tggctattat
661 aatttagaag tcattagcga gcaggctacc gcatacgtta tcaaaataaa cgaactaaaa
721 gaactactga gcaaaaatct tacgcacttt ttctatgttt tccaaaccct acaaaaacaa
781 gtttcataca gtctagctaa atttaatgat ttttcgatta acgggaagct tggctctatt
841 tgcggtcaac ttttaatcct gacctatgtg tatggtaaag aaactcctga tggcatcaag
901 attacactgg ataatttaac aatgcaggag ttaggatatt caagtggcat cgcacatagc
961 tcagctgtta gcagaattat ttccaaatta aagcaagaga aagttatcgt gtataaaaat
1021 tcatgctttt atgtacaaaa tcttgattat ctcaaaagat atgcccctaa attagatgaa
1081 tggttttatt tagcatgtcc tgctacttgg gaaaattaaa ttaaatcaaa aacagtattc
1141 ctcaatgagg aatactgttt tatattttat tcgaataaag aacttacaga agcattttca
1201 tgaacgcgta cgattgcttc accaagaaga gctgcaacag atagttgttc cattttgtca
1261 atccattttt cttctggaag agcgatggag tttgtaacaa ctaatttttc gattggcgat
1321 tcttcaatac gtttcatagc cgacctgaaa gaactgggtg cgaacaacat gcgtaaactt
1381 ttgttgcgcc agctataacc accgcaattc ctggagttat tttttctacc aagagaagtg
1441 gcaacataat aatcgtcgcg ataatcgata ctaaaaagcc aatcgcaaca attattaaaa
1501 cccattttag taacatgaaa aataagtgct tttgcgggta tttccaactt tttcggatag
//