GenBank-Updates@genbank.bio.net (01/25/91)
LOCUS MZEFEGLU 5617 bp ss-mRNA PLN 25-JAN-1991
DEFINITION Zea mays ferredoxin-dependent glutamate synthase mRNA, complete
cds.
ACCESSION M59190 J05739
KEYWORDS ferredoxin-dependent glutamate synthase.
SOURCE Zea mays seedlings, cDNA to mRNA.
ORGANISM Zea mays
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
Commelinidae; Cyperales; Poaceae.
REFERENCE 1 (bases 1 to 5617)
AUTHORS Sakakibara,H., Watanabe,M., Hase,T. and Sugiyama,T.
TITLE Molecular cloning and characterization of complementary DNA
encoding for ferredoxin-dependent glutamate synthase in maize leaf
JOURNAL J. Biol. Chem. 266, 2028-2035 (1991)
STANDARD unannotated staff_entry
FEATURES Location/Qualifiers
CDS 328..5178
/EC_number="1.4.7.1"
/codon_start=328
/product="ferredoxin-dependent glutamate synthase"
sig_peptide 328..618
/EC_number="1.4.7.1"
/codon_start=328
/product="ferredoxin-dependent glutamate synthase"
mat_peptide 619..5175
/EC_number="1.4.7.1"
/evidence=EXPERIMENTAL
/codon_start=619
/product="ferredoxin-dependent glutamate synthase"
BASE COUNT 1478 a 1241 c 1440 g 1458 t
ORIGIN
1 ccgcctctca cctctccagt ccccaccccc gcccccgtcc gctccctatc ctgttcgttc
61 ccctcctctc cacacgatcc cctcccttgt cggcttccgc gcgggcggga gtggccgcgg
121 ccacgactcc accgcctcct gctcgcgcca ccacccctcg gcccctcgcg gcgccgcgcg
181 accggcgcgt ggtgcgcgtt tgcctgcccg tcgtcggccc cttcttatta gttattatta
241 ggcggaggag aggctagctc tgcggggggt cgccgcgcgc cgccgaatcg gatcacacgc
301 ctccccttcc cgccaggcca gggggccatg gccacgctcc cgcgcgccgc gccgcccacg
361 cccgccgcgc tgctcccgct gccccgcgcc gcgccgccgc tgctgctcgc cgggagggcc
421 gccgccgcgc gccgctctcg gctgcgcgcg cggggcccgt ccgcggcggc gcgccggagc
481 tgggtcgtcg cctccgccgc ttcctcctcc tcacgggccg tggtcggcgg cgtcgcccgc
541 cgggaggccc ctcccgcgcc gcagaagccc acgcagcagg ctgcggactt gaaccatatc
601 ttgtctgaac gtggagcttg tggtgttgga tttgtcgcaa acttgaaaaa tatgtcttca
661 tttgacattg ttcgtgatgc tcttatggct cttgggtgca tggagcatcg tggtggttgt
721 ggagcggaca gtgactcagg tgatggtgca ggactgatga gtgccgttcc atgggacttg
781 tttgatgact gggccagcaa gcaagggctt gctctctttg acagaagaaa tacaggtgta
841 ggcatggtct tccttccaca agatgagaag tcaatggaag aagccaaagc tgcaactgaa
901 aaggtttttg tggatgaagg ccttgaggtt cttggttgga gacccgttcc ttttaatgta
961 tcagttgtag gtcgcaatgc aaaagaaacc atgcctaata tacaacaaat atttgtgaaa
1021 gttgcaaaag aagataatgc tgatgacata gagagagaat tatacatcag ccgaaagctg
1081 atagagcggg ctgcaaaatc ttttagttgg gcagatgaac tttatttctg ctctttatca
1141 agcagaacga ttgtctacaa gggaatgcta cgatctgagg ttcttgggca attctattta
1201 gaccttcaga atgaactgta taaatcacct tttgccatat atcatcgaag attcagtaca
1261 aatacaagtc ctcgatggcc tcttgctcaa cctatgaggt tacttggaca caacggagaa
1321 attaacacaa tacaggggaa cttgaactgg atgcgatcaa gggaaaccac actaaagtct
1381 cctgtatggc gaggtcgtga gcatgaaatt tgcccgtttg gtgaccctaa agcatctgat
1441 tcagcgaatc ttgacagtac agctgaattg ctgctaagaa gtggtaggag tcctgcagaa
1501 gctcttatga ttctcgtccc tgaggcatat aagaaccatc caacattatc tataaaatac
1561 cccgaggtaa ctgactttta tgactactac aaaggacaaa tggaagcttg ggatggacct
1621 gctttacttc tatttagtga tgggaggact gtaggagcaa ctcttgatag gaatggtctc
1681 cgtccagcac gctattggag aacgtcagat gacttcgttt atgtcgcatc tgaagttggt
1741 gttataccaa tggatgagtc aaaagtagta atgaaaggac gattaggtcc tggaatgatg
1801 ataactgttg atctacagac tggacaggtg cttgaaaaca cagaagtaaa gaaaactgtg
1861 gcttcagcga gcccctatgg gacttggttg caagaatgca cacgtttgat aaagcctgtc
1921 aacttcctct cttcaactat catggacaat gagactgttt tgagacatca acaggcattt
1981 ggctattcaa gtgaagatgt gcaaatggtg attgaatcaa tggcttcaca aggaaaggaa
2041 ccaacatttt gtatgggcga tgacattcca ttagctgtat tatcacaaag accacatttg
2101 ttatatgatt atttcaagca gcggtttgcg caggttacaa atcctgcaat tgatcccctt
2161 agagaaggtt tagttatgtc tcttgaggtt aatattggga aaagaggcaa catcttggag
2221 gttggccctg aaaatgctga tcaggttgca ctatcaagcc ctgtgctgaa tgaaggtgaa
2281 ctggaaactc tattgaacga ctcaaaactt aagcccaaag tactgtcaac atacttcgat
2341 attcgtaaag gtctagatgg ttcacttgat aagacaattc aggctctttg tgaagaagct
2401 gatgctgctg tgcggagtgg ctctcaactt cttgttcttt ctgatcgttc tgaagcacct
2461 gaacctactc gtcctgccat accaatactt ctagctgttg gcgccatcca ccagcatttg
2521 attcagaatg gtctccgtat gtctgcttct attgttgctg atactgctca gtgttttagc
2581 acacatcatt ttgcctgtct gatcggatat ggtgccagtg ctgtgtgtcc ttatctggca
2641 ctagaaacat gccggcaatg gaggctgagc aacaaaactc ttaatttgat gcggaatggg
2701 aagatgccca cagtgaccat cgagcaggca caaaggaact ttatcaaggc agtaaaatca
2761 ggtctgctca aaattctttc aaaaatgggt atctcactgc tctcaagcta ttgtggagct
2821 cagatctttg aaatttatgg tcttggtcaa gaagttgttg atcttgcctt ctgtggaagt
2881 gtatcgaaaa ttggaggtct cacacttgat gagctgggta gggagacctt atcattctgg
2941 gtgaaggcgt tctctgaaga tactgcaaag aggctagaaa actttggatt tatccaatcc
3001 agacctggag gtgaatacca tgcaaataat cctgagatgt ccaagctgtt gcacaaagca
3061 attcgcgaga aacgtgataa tgcgtacact gtctaccaac aacatcttgc aagccgtcct
3121 gtcaatgttc ttcgagatct tcttgaattg aaaagtgatc gtgcacctat tcctattggc
3181 aaagtggagt ctgcaacctc cattgttgaa cgtttttgta ctggtggaat gtccctagga
3241 gccatttcaa gggaaacgca tgaagctatt gcaattgcaa tgaatagaat aggtgggaaa
3301 tctaattcag gagaaggtgg tgaggatcca atccgttgga atcccctcac tgatgttgta
3361 gatggttatt ctccaacact tcctcatctc aagggtcttc agaatggtga cacagccaca
3421 agtgccatta agcaggttgc atctggacgt tttggagtta caccgacatt cttagtaaat
3481 gctgaccaaa ttgagataaa gattgcgcaa ggtgcaaagc ctggtgaagg aggtcaactt
3541 cctgggaaaa aagtcagtgc atacattgct cgtctgagga actccaaacc tggagttcct
3601 ctaatctccc cgcctccaca ccatgacatt tattctatcg aggatcttgc acagttgatt
3661 tatgacctcc atcagataaa tcctaaagct aaagtatctg taaagcttgt gtctgaagct
3721 ggaattggaa ctgtagcttc tggagtttca aaggcgaatg cagacataat tcagatatca
3781 ggtcatgatg gtggtactgg agctagccca atcagctcca tcaaacatgc tgggggtccg
3841 tgggaacttg gtcttacaga aacaaatcag acacttatac aaaatggatt gagagagagg
3901 gtagtactta gggtggacgg tggattcagg agtggtcaag atgttcttat tgctgctgct
3961 atgggagctg atgaatatgg ctttggttct gtagccatga tagctactgg atgtgtcatg
4021 gcacggattt gtcacacaaa taattgtcca gttggagtgg ctagtcagag agaagagctt
4081 cgtgcaaggt ttcctggtgt tcctggtgat cttgttaact acttcctctt tgttgccgag
4141 gaggttcgag ctgcattagc ccagttgggt tatgagaagt tggatgacat cattgggcga
4201 acagatttgc ttaaaccaaa gcacatctca ttggtgaaaa cgcagcacat tgatcttgga
4261 tacctcctat caaatgctgg attgcctgaa tggagcagct cccaaattag gagccaggac
4321 gtccacacta atggccctgt tcttgatgag acaatccttg cagatcctga gatagctgat
4381 gctattgaga atgagaagga agtttccaag gcatttcaaa tctataatgt tgacagagct
4441 gtctgtggtc gggtagcagg tgtgattgcc aagaagtatg gagacacagg ttttgctggc
4501 cagctcaaca tcacgttcaa tggaagtgca gggcagtcct ttggttgttt cctgactcct
4561 ggaatgaaca ttcggctagt tggggaggcc aatgattatg ttggaaaggg tatggctggt
4621 ggagaactgg tggtagttcc tgtagacaaa acaggatttg ttcctgagga tgctactata
4681 gttggaaaca cttgtctgta tggagctaca ggtggtcagg tatttgtgag aggcaaggca
4741 ggagaaagat ttgctgttag aaactctcta tgtcaggcag tggttgaggg caccggagat
4801 cattgctgtg agtacatgac tggtggctgc gtagtcgtac ttggcaaagc tgggaggaat
4861 gttgcagctg gaatgactgg tggtcttgcc tacattctag atgaggatga tacacttgtc
4921 ccaaaggtca acaaggaaat cgtcaaaatg cagagagtga atgctccagc tgggcagatg
4981 cagctaaagg gcttgattga ggcctatgtt gaaaaaacgg gcagcgagaa aggtattgcg
5041 attttgaggg aatgggaggc ctatttgccg ctcttctggc aactggtacc gcccagtgaa
5101 gaagactcgc ctgaggcttg tgcagagttt gagagagtac ttgccaagca agcaaccacc
5161 caattgtctg cgaagtgatc gaatcagact acaggaaggt ggtaggtgtt ttctatttga
5221 cacaaagtga tctcatctta tcacttcaag aaaatcgaag accataaccg gcactctgag
5281 gcaccgaagt gcccgtctga agactgcaac tttgtggagg ctctgcccag gccatctttt
5341 tgagttgagc taacagaacc gagggttctt ctcgacgctc gtgcacctag gtaacccctg
5401 taaattggtt tgctgacgct aggctgcaag gggcctgccc tgtttgaact gctgtactgt
5461 acatgattct tggccgttgc cctgtgtaat tatgttttgg ttagctcgta aatttatacg
5521 catccaatgg aggcatacaa tgcaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
5581 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa
//GenBank-Updates@genbank.bio.net (03/07/91)
LOCUS MZEFEGLU 5617 bp ss-mRNA PLN 07-MAR-1991
DEFINITION Zea mays ferredoxin-dependent glutamate synthase mRNA, complete
cds.
ACCESSION M59190 J05739
KEYWORDS ferredoxin-dependent glutamate synthase.
SOURCE Zea mays (cv. Golden Cross Bantam T51) seedling, cDNA to mRNA.
ORGANISM Zea mays
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Liliopsida;
Commelinidae; Cyperales; Poaceae.
REFERENCE 1 (bases 1 to 5617)
AUTHORS Sakakibara,H., Watanabe,M., Hase,T. and Sugiyama,T.
TITLE Molecular cloning and characterization of complementary DNA
encoding for ferredoxin-dependent glutamate synthase in maize leaf
JOURNAL J. Biol. Chem. 266, 2028-2035 (1991)
STANDARD unannotated staff_entry
FEATURES Location/Qualifiers
CDS 328..5178
/EC_number="1.4.7.1"
/product="ferredoxin-dependent glutamate synthase"
/gene="glutamate synthase"
/codon_start=328
sig_peptide 328..618
/product="ferredoxin-dependent glutamate synthase"
/codon_start=328
mat_peptide 619..5175
/product="ferredoxin-dependent glutamate synthase"
/evidence=EXPERIMENTAL
/codon_start=619
BASE COUNT 1478 a 1241 c 1440 g 1458 t
ORIGIN
1 ccgcctctca cctctccagt ccccaccccc gcccccgtcc gctccctatc ctgttcgttc
61 ccctcctctc cacacgatcc cctcccttgt cggcttccgc gcgggcggga gtggccgcgg
121 ccacgactcc accgcctcct gctcgcgcca ccacccctcg gcccctcgcg gcgccgcgcg
181 accggcgcgt ggtgcgcgtt tgcctgcccg tcgtcggccc cttcttatta gttattatta
241 ggcggaggag aggctagctc tgcggggggt cgccgcgcgc cgccgaatcg gatcacacgc
301 ctccccttcc cgccaggcca gggggccatg gccacgctcc cgcgcgccgc gccgcccacg
361 cccgccgcgc tgctcccgct gccccgcgcc gcgccgccgc tgctgctcgc cgggagggcc
421 gccgccgcgc gccgctctcg gctgcgcgcg cggggcccgt ccgcggcggc gcgccggagc
481 tgggtcgtcg cctccgccgc ttcctcctcc tcacgggccg tggtcggcgg cgtcgcccgc
541 cgggaggccc ctcccgcgcc gcagaagccc acgcagcagg ctgcggactt gaaccatatc
601 ttgtctgaac gtggagcttg tggtgttgga tttgtcgcaa acttgaaaaa tatgtcttca
661 tttgacattg ttcgtgatgc tcttatggct cttgggtgca tggagcatcg tggtggttgt
721 ggagcggaca gtgactcagg tgatggtgca ggactgatga gtgccgttcc atgggacttg
781 tttgatgact gggccagcaa gcaagggctt gctctctttg acagaagaaa tacaggtgta
841 ggcatggtct tccttccaca agatgagaag tcaatggaag aagccaaagc tgcaactgaa
901 aaggtttttg tggatgaagg ccttgaggtt cttggttgga gacccgttcc ttttaatgta
961 tcagttgtag gtcgcaatgc aaaagaaacc atgcctaata tacaacaaat atttgtgaaa
1021 gttgcaaaag aagataatgc tgatgacata gagagagaat tatacatcag ccgaaagctg
1081 atagagcggg ctgcaaaatc ttttagttgg gcagatgaac tttatttctg ctctttatca
1141 agcagaacga ttgtctacaa gggaatgcta cgatctgagg ttcttgggca attctattta
1201 gaccttcaga atgaactgta taaatcacct tttgccatat atcatcgaag attcagtaca
1261 aatacaagtc ctcgatggcc tcttgctcaa cctatgaggt tacttggaca caacggagaa
1321 attaacacaa tacaggggaa cttgaactgg atgcgatcaa gggaaaccac actaaagtct
1381 cctgtatggc gaggtcgtga gcatgaaatt tgcccgtttg gtgaccctaa agcatctgat
1441 tcagcgaatc ttgacagtac agctgaattg ctgctaagaa gtggtaggag tcctgcagaa
1501 gctcttatga ttctcgtccc tgaggcatat aagaaccatc caacattatc tataaaatac
1561 cccgaggtaa ctgactttta tgactactac aaaggacaaa tggaagcttg ggatggacct
1621 gctttacttc tatttagtga tgggaggact gtaggagcaa ctcttgatag gaatggtctc
1681 cgtccagcac gctattggag aacgtcagat gacttcgttt atgtcgcatc tgaagttggt
1741 gttataccaa tggatgagtc aaaagtagta atgaaaggac gattaggtcc tggaatgatg
1801 ataactgttg atctacagac tggacaggtg cttgaaaaca cagaagtaaa gaaaactgtg
1861 gcttcagcga gcccctatgg gacttggttg caagaatgca cacgtttgat aaagcctgtc
1921 aacttcctct cttcaactat catggacaat gagactgttt tgagacatca acaggcattt
1981 ggctattcaa gtgaagatgt gcaaatggtg attgaatcaa tggcttcaca aggaaaggaa
2041 ccaacatttt gtatgggcga tgacattcca ttagctgtat tatcacaaag accacatttg
2101 ttatatgatt atttcaagca gcggtttgcg caggttacaa atcctgcaat tgatcccctt
2161 agagaaggtt tagttatgtc tcttgaggtt aatattggga aaagaggcaa catcttggag
2221 gttggccctg aaaatgctga tcaggttgca ctatcaagcc ctgtgctgaa tgaaggtgaa
2281 ctggaaactc tattgaacga ctcaaaactt aagcccaaag tactgtcaac atacttcgat
2341 attcgtaaag gtctagatgg ttcacttgat aagacaattc aggctctttg tgaagaagct
2401 gatgctgctg tgcggagtgg ctctcaactt cttgttcttt ctgatcgttc tgaagcacct
2461 gaacctactc gtcctgccat accaatactt ctagctgttg gcgccatcca ccagcatttg
2521 attcagaatg gtctccgtat gtctgcttct attgttgctg atactgctca gtgttttagc
2581 acacatcatt ttgcctgtct gatcggatat ggtgccagtg ctgtgtgtcc ttatctggca
2641 ctagaaacat gccggcaatg gaggctgagc aacaaaactc ttaatttgat gcggaatggg
2701 aagatgccca cagtgaccat cgagcaggca caaaggaact ttatcaaggc agtaaaatca
2761 ggtctgctca aaattctttc aaaaatgggt atctcactgc tctcaagcta ttgtggagct
2821 cagatctttg aaatttatgg tcttggtcaa gaagttgttg atcttgcctt ctgtggaagt
2881 gtatcgaaaa ttggaggtct cacacttgat gagctgggta gggagacctt atcattctgg
2941 gtgaaggcgt tctctgaaga tactgcaaag aggctagaaa actttggatt tatccaatcc
3001 agacctggag gtgaatacca tgcaaataat cctgagatgt ccaagctgtt gcacaaagca
3061 attcgcgaga aacgtgataa tgcgtacact gtctaccaac aacatcttgc aagccgtcct
3121 gtcaatgttc ttcgagatct tcttgaattg aaaagtgatc gtgcacctat tcctattggc
3181 aaagtggagt ctgcaacctc cattgttgaa cgtttttgta ctggtggaat gtccctagga
3241 gccatttcaa gggaaacgca tgaagctatt gcaattgcaa tgaatagaat aggtgggaaa
3301 tctaattcag gagaaggtgg tgaggatcca atccgttgga atcccctcac tgatgttgta
3361 gatggttatt ctccaacact tcctcatctc aagggtcttc agaatggtga cacagccaca
3421 agtgccatta agcaggttgc atctggacgt tttggagtta caccgacatt cttagtaaat
3481 gctgaccaaa ttgagataaa gattgcgcaa ggtgcaaagc ctggtgaagg aggtcaactt
3541 cctgggaaaa aagtcagtgc atacattgct cgtctgagga actccaaacc tggagttcct
3601 ctaatctccc cgcctccaca ccatgacatt tattctatcg aggatcttgc acagttgatt
3661 tatgacctcc atcagataaa tcctaaagct aaagtatctg taaagcttgt gtctgaagct
3721 ggaattggaa ctgtagcttc tggagtttca aaggcgaatg cagacataat tcagatatca
3781 ggtcatgatg gtggtactgg agctagccca atcagctcca tcaaacatgc tgggggtccg
3841 tgggaacttg gtcttacaga aacaaatcag acacttatac aaaatggatt gagagagagg
3901 gtagtactta gggtggacgg tggattcagg agtggtcaag atgttcttat tgctgctgct
3961 atgggagctg atgaatatgg ctttggttct gtagccatga tagctactgg atgtgtcatg
4021 gcacggattt gtcacacaaa taattgtcca gttggagtgg ctagtcagag agaagagctt
4081 cgtgcaaggt ttcctggtgt tcctggtgat cttgttaact acttcctctt tgttgccgag
4141 gaggttcgag ctgcattagc ccagttgggt tatgagaagt tggatgacat cattgggcga
4201 acagatttgc ttaaaccaaa gcacatctca ttggtgaaaa cgcagcacat tgatcttgga
4261 tacctcctat caaatgctgg attgcctgaa tggagcagct cccaaattag gagccaggac
4321 gtccacacta atggccctgt tcttgatgag acaatccttg cagatcctga gatagctgat
4381 gctattgaga atgagaagga agtttccaag gcatttcaaa tctataatgt tgacagagct
4441 gtctgtggtc gggtagcagg tgtgattgcc aagaagtatg gagacacagg ttttgctggc
4501 cagctcaaca tcacgttcaa tggaagtgca gggcagtcct ttggttgttt cctgactcct
4561 ggaatgaaca ttcggctagt tggggaggcc aatgattatg ttggaaaggg tatggctggt
4621 ggagaactgg tggtagttcc tgtagacaaa acaggatttg ttcctgagga tgctactata
4681 gttggaaaca cttgtctgta tggagctaca ggtggtcagg tatttgtgag aggcaaggca
4741 ggagaaagat ttgctgttag aaactctcta tgtcaggcag tggttgaggg caccggagat
4801 cattgctgtg agtacatgac tggtggctgc gtagtcgtac ttggcaaagc tgggaggaat
4861 gttgcagctg gaatgactgg tggtcttgcc tacattctag atgaggatga tacacttgtc
4921 ccaaaggtca acaaggaaat cgtcaaaatg cagagagtga atgctccagc tgggcagatg
4981 cagctaaagg gcttgattga ggcctatgtt gaaaaaacgg gcagcgagaa aggtattgcg
5041 attttgaggg aatgggaggc ctatttgccg ctcttctggc aactggtacc gcccagtgaa
5101 gaagactcgc ctgaggcttg tgcagagttt gagagagtac ttgccaagca agcaaccacc
5161 caattgtctg cgaagtgatc gaatcagact acaggaaggt ggtaggtgtt ttctatttga
5221 cacaaagtga tctcatctta tcacttcaag aaaatcgaag accataaccg gcactctgag
5281 gcaccgaagt gcccgtctga agactgcaac tttgtggagg ctctgcccag gccatctttt
5341 tgagttgagc taacagaacc gagggttctt ctcgacgctc gtgcacctag gtaacccctg
5401 taaattggtt tgctgacgct aggctgcaag gggcctgccc tgtttgaact gctgtactgt
5461 acatgattct tggccgttgc cctgtgtaat tatgttttgg ttagctcgta aatttatacg
5521 catccaatgg aggcatacaa tgcaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
5581 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa
//