GenBank-Updates@genbank.bio.net (03/07/91)
LOCUS FVBCRE 1955 bp ds-DNA BCT 07-MAR-1991
DEFINITION Flavobacterium sp. creatinase gene.
ACCESSION D00656
KEYWORDS creatinase; creatine amidinohydrolase.
SOURCE Flavobacterium sp. (strain U-188) genomic DNA, clone pKLS501.
ORGANISM Flavobacterium sp.
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Neisseriaceae.
REFERENCE 1 (bases 1 to 1955)
AUTHORS Koyama,Y., Kitao,S., Otake,H., Suzuki,M. and Nakano,E.
TITLE Cloning and expression of the creatinase gene from Flavobacterium
sp. U-188 in Escherichia coli
JOURNAL Agric. Biol. Chem. 54, 1453-1457 (1990)
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by: Yasuji
Koyama Kikkoman Corporation Research and Development Division 399
Noda Noda-shi Chiba 278 Japan Phone: 0471-23-5571
FEATURES Location/Qualifiers
repeat_region 339..345
/note="inverted repeat"
repeat_region 352..358
/note="inverted repeat"
misc_binding 574..579
/note="pot. SD sequence"
CDS 590..1726
/note="creatinase (EC 3.5.3.3)"
repeat_region 1808..1820
/note="inverted repeat"
repeat_region 1823..1836
/note="inverted repeat"
BASE COUNT 380 a 677 c 571 g 327 t
ORIGIN
1 gatcgggcgc atggaggcca acctccagga gccgatcgag ctggacgagc tggccgaggt
61 catccagctg tcgcgccgtc acctcgagcg gctgtttcgc aaatacctgc gctgctcgcc
121 ctcgcgctac tacatgagct cgcctgctgc gcgcccgcca gctgttgcgc cagaccgagt
181 tgccggtggt gcaggtcgcg gcgaagctgc ggtttccatt cggcgcagac cttctccaag
241 agttaccgcg aacatttcgg catgcgccca gtggcgagcg tgtcgaccga tccctgctgt
301 gtcgcgcagc cgtgcactga gcgccagctt cccgttggcc cgcctcctcg taggcgggtt
361 ttttttgccc gttcgtcagc ccctctcagg cgctgcagcc cagtggcgac gggcctgccg
421 gcctgccgtg atggccttat gaccccgccc gggccgttcg gcagcccagc gaatctgacc
481 aacttgaggt cggatacgtg caaaaggccc ccgggggttc tgttgcaatg tcttcaacgg
541 cagccagtcc ggtgtgccag atcagtgaat aacaagaagg gttctcgtca tgcaaatgcc
601 caaaacactc cgcatccgca acggtgaaaa ggtcaagtcc accttctccg cgcaggaata
661 cgccaaccgc catgccaagc tgcgcgcgca cctggcggcc gagaacatcg atgctgcggt
721 cttcacctcg taccacaaca tcaactacta ctccgacttc ctgtactgct ccttcggtcg
781 tccctacgcc ctggtcgtga cgcaggacga cgtgatcagc atcagcgcca acatcgacgg
841 tggccagccg tggcgccgca ccgtgggcac cgacaacatc gtctacaccg actggcagcg
901 cgacaactac ttcgtcgcca tccagcaggc gctgcccagg gcgcgtcgca tcggtatcga
961 gcacgaccac ctgaacctgc agaaccgcga caagctggcc gcccgctacc cggatgccga
1021 gctggtcgac gtggccgccg cctgcatgcg catgcgcatg atcaagtcgg ctgaagagca
1081 cgagatgatc cgccatggcg cgcgcgtcgc cgacatcggt ggcgcggcca tcgtcgaggc
1141 cctgcgtgat caggtgccgg agtacgaagt ggcgctgcac gcgacccagg ccatggtgcg
1201 cgccattgcc gagaccttcg acaacgtcga gctgatggac acctggacct ggttccagtc
1261 gggcatcaac accgacggcg cgcacaaccc ggtgaccacc cgcaaggtca acaagggcga
1321 catcctcagc ctgaactgct tcccgatgat cgccggttac tacaccgcgc tggagcgtac
1381 cctgttcctc gaccactgct ccgacgacca cctgcgcatg tggcaggcca acgtcgaagt
1441 gcacgaggcc ggcctcaagc tgatcaaacc gggcatgcgc tgcagcgaca tcgccaagga
1501 gctcaacgag atcttcctca agcacgatct gctgcagtac cgcaccttcg gttacggtca
1561 ctccttcggc accctgagcc actactacgg ccgcgacggg cctggagctg cgcgaggaca
1621 tcgacaccgt tctggagccg ggcatggtgg tctccatgga gccgatgatc atgctgcccg
1681 aaggtcgtcc gggcgccggt ggcgtaccgc gagcacgaca tcctgatcgt caacgagaac
1741 ggtgccgaga acatcaccaa gttcccgtac ggtccggaga gaaacatcat ccgcaaataa
1801 ccggtgcgcc ccgcagggtc aggacctgcg cggggcaact ggctgcaaca cgcaaaaccg
1861 aacctccgtg ccacacgcct ggaagcccgc ttcccgtcga ctgcgctttg actccccgtt
1921 gccgaacaca acaacaagag gaacaggcga tgatc
//