GenBank-Updates@genbank.bio.net (03/07/91)
LOCUS RATIGF1A 1052 bp ss-mRNA ROD 07-MAR-1991
DEFINITION Rat insulin-like growth factor I mRNA.
ACCESSION D00698
KEYWORDS insulin-like growth factor I.
SOURCE Rat liver, cDNA to mRNA.
ORGANISM Rattus sp.
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (sites)
AUTHORS Shimatsu,A. and Rotwein,P.
TITLE Mosaic evolution of the insulin-like growth factors: Organization,
sequence, and expression of the rat insulin-like growth factor I
gene
JOURNAL J. Biol. Chem. 262, 7894-7900 (1987)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 1052)
AUTHORS Kato,H., Okoshi,A., Miura,Y. and Noguchi,T.
TITLE A new cDNA clone relating to larger molecular species of rat
insulin-like growth factor-I mRNA
JOURNAL Agric. Biol. Chem. 54, 1599-1601 (1990)
STANDARD full staff_entry
COMMENT
[J. Biol. Chem. 262, 7894-7900 (1987)] sites.
The difference in the size of IGF-I mRNA has been suggested to be
primarily due to differences in the 3'-untranslated region in [J.
Biol. Chem. 262, 7894-7900 (1987)].
The cDNA clone to IGF-I mRNA reported in [Agric. Biol. Chem. 54,
1599-1601 (1990)] gives direct evidence
for this hypothesis.
FEATURES Location/Qualifiers
CDS 43..426
/note="insulin-like growth factor I precursor"
mat_peptide 109..318
/note="insulin-like growth factor mature peptide"
variation 606..606
/note="g in [Agric. Biol. Chem. 54, 1599-1601 (1990)];
deletion in [J. Biol. Chem. 262, 7894-7900 (1987)]"
variation 625..625
/note="c in [Agric. Biol. Chem. 54, 1599-1601 (1990)]; a
in [J. Biol. Chem. 262, 7894-7900 (1987)]"
variation 645..645
/note="a in [Agric. Biol. Chem. 54, 1599-1601 (1990)];
deletion in [J. Biol. Chem. 262, 7894-7900 (1987)]"
misc_signal 710..715
/note="polyadenylation signal"
misc_feature 731..731
/note="polyadenylation site (putative)"
misc_feature 1023..1023
/note="polyadenylation site (putative)"
BASE COUNT 301 a 256 c 225 g 270 t
ORIGIN 788 bp upstream of AvaIII site.
1 ggacgtacca aaatgagcgc acctccaata aagatacaca tcatgtcgtc ttcacatctc
61 ttctacctgg cactctgctt gctcaccttt accagctcgg ccacagccgg accagagacc
121 ctttgcgggg ctgagctggt ggacgctctt cagttcgtgt gtggaccaag gggcttttac
181 ttcaacaagc ccacaggcta tggctccagc attcggaggg caccacagac gggcattgtg
241 gatgagtgtt gcttccggag ctgtgatctg aggaggctgg agatgtactg tgctccgctg
301 aagcctacaa agtcagctcg ttccatccgg gcccagcgcc acactgacat gcccaagact
361 cagaaggaag tacacttgaa gaacacaagt agaggaagtg caggaaacaa gacctacaga
421 atgtaggagg agcctcccga ggaacagaaa atgccacgtc accgcaagat cctttgctgc
481 ttgagcaacc tgcaaaacat cggaacacct gccaaatatc aataatgagt tcaataccat
541 ttcagagatg ggcatttccc tcaatgaaat acacaagtaa acattccgac attgtcttta
601 ggagtggttt gttaaaaaaa aaaacaaaaa acaaaaacaa aaacaaaaaa aaagcttgca
661 ccttgcaaaa gtggtcctgg cgtgggtaga ttgctgttaa tcctttatca ataacgttct
721 atagagaata tataaatata tatataatta tatctcctag tccctgcctc ttaagagccg
781 aaaatgcatg ggtgttgtag acattcggtt gcactaaatt cctctctgaa ttttggctgc
841 tgaagccgtt catttagcaa ctgtttatag gtggttgatg aatggttcct tatctccatt
901 tcttcctatg tagcttaagc cgcttccttc acagaatcta ataatctcgt ctaggccatt
961 agccctgccc tttcttaaca ttcttgtatt tgttgaattt ggcctcctcg aaagcaatag
1021 caactgggtg gcccacccaa gttttaacgc cc
//