GenBank-Updates@genbank.bio.net (01/24/91)
LOCUS ECOTRAX 1413 bp ds-DNA BCT 24-JAN-1991
DEFINITION Plasmid F (from Escherichia coli K-12) conjugative transfer
co-repressor (finO) and traX genes, complete cds, and DNA helicase
(traI), 3' end.
ACCESSION M38047
KEYWORDS DNA helicase; conjugative transfer co-repressor; finO gene;
traI gene; traX gene.
SOURCE Plasmid F (from E.coli K12) DNA.
ORGANISM Plasmid F
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 1413)
AUTHORS Cram,D.S., Loh,S.M., Cheah,K.C. and Skurray,R.A.
TITLE Nucleotide sequence and evolution of the distal end of the transfer
region on plasmids F and R6-5: Identification of new open reading
frames
JOURNAL Unpublished (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by S.M.Loh, 28-AUG-1990.
Monash University
Dept Microbiology
Wellington Road
Clayton, Melbourne
Victoria, Australia 3168
FEATURES Location/Qualifiers
CDS 1..33
/gene="traI"
/codon_start=1
/partial
/product="DNA helicase"
RBS 35..43
/gene="traX"
CDS 53..799
/gene="traX"
/codon_start=53
/product="TraX protein"
RBS 834..842
/gene="finO"
CDS 853..1413
/note="This gene has been inactivated by the insertion of
IS3 between nt 1248 and 1249"
/gene="finO"
/codon_start=853
/product="conjugative transfer co-repressor"
misc_signal 1246..1248
/note="IS3 insertion target sequence"
BASE COUNT 303 a 357 c 449 g 304 t
ORIGIN
1 ctgcagaaag agaaaaccct gggtggagac tgatacagga agacaaacgc tgatgacaac
61 ggataacacg aacacgacac gtaacgattc actggttgcc cggaccgata cctggttgca
121 gtcgttgctg gtctggtcac ccggacagcg ggatatcatc aaaacggtgg cactggtgct
181 gatggttctg gaccatgcaa accgtattct gcatctggac cagtcgtgga tgtttctagc
241 cgggcgcggg gcctttccgc tgtttgccct ggtgtggggg ctgaatctgt cccgtcatgc
301 gcatatccgg caactggcca ttaaccggct gtggggatgg gcagtttttt cccagttctg
361 gtattacttt gccggctttc cctggtatga ggggaatatc ctgtttgcct ttgcagtggc
421 ggcacaggtg ctgacatggt gtgaaacgcg cagtgggtgg cgtactgcag cggccattct
481 catgatggca ctgtgggggc ctttgtccgg caccagttac ggcattgccg ggctgctgat
541 gctggctgtc agccaccggc tgtaccgggc ggaagacaga atggaacgtc tggtgcttgt
601 cgcttgcctg ctggccgtga ttccggcgct taaccttgcc accagtgatg cggcggctgt
661 agccggcctt gtgatgacgg tgctgaccgt tggtctggtg tcgtgtgcag ggaaattatt
721 atcccgtttc tggcccggag actttttccc gacgttctat gcctgtcatc tggctgtgct
781 gggcgttctg gcgctgtgac gggtgtggta tctttggccg caagaggtga ttcgtcagag
841 gcagaacaca gcatgacaga gcagaagcga ccggtactga cactgaagcg gaagacagaa
901 ggggagacgc ttgtccgcag ccggaaaacc atcatcaatg tcaccacgcc accaaagtgg
961 aaggtgaaaa agcagaagct ggccgagaaa gccgcccggg aagcagagct ggcggcaaaa
1021 aaagcgcagg ccagacaggc gctgtccatt tatctgaatc tgcccacgct ggatgatgcc
1081 gtgaacaccc tgaagccctg gtggccggga ttatttgacg gtgacacgcc ccggcttctg
1141 gcctgcggta tccgggacgt gttactggaa gacgtggcgc agcggaatat cccgctctcg
1201 cataaaaaac tgcgcagggc gctgaaggcc atcacccgtt cagaaagcta tctgtgtgcc
1261 atgaaagccg gtgcctgccg gtatgacacg gaagggtatg tgacggagca tatttctcag
1321 gaggaggaag cgtatgcggc agagcgtctg gataaaatcc gccgccagaa ccggataaag
1381 gcagaacttc aggccgtgct tgatgagaaa taa
//GenBank-Updates@genbank.bio.net (03/08/91)
LOCUS FPLTRAH 4847 bp ds-DNA BCT 08-MAR-1991
DEFINITION Plasmid F (from E.coli) trbF, traH, and traG genes, complete cds,
and traS gene, 5' end.
ACCESSION M59763
KEYWORDS F pilus assembly.
SOURCE Plasmid F DNA.
ORGANISM Plasmid F
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 4847)
AUTHORS Ham,L.M., Firth,N. and Skurray,R.
TITLE Nucleotide sequence of the F plasmid transfer gene, traH:
identification of a new gene and a promoter within the transfer
operon
JOURNAL Gene 75, 157-165 (1989)
STANDARD simple staff_entry
REFERENCE 2 (bases 1 to 4847)
AUTHORS Firth,N. and Skurray,R.A.
JOURNAL Unpublished (1990)
STANDARD simple staff_entry
COMMENT Base pairs 1 to 1671 appear in Gene, volume 75, pages 157-165,
whereas base pairs 1666 to 4847 are in preparation.
FEATURES Location/Qualifiers
CDS 173..553
/gene="trbF"
/codon_start=173
CDS 550..1926
/gene="traH"
/evidence=EXPERIMENTAL
/codon_start=550
sig_peptide 550..621
/gene="traH"
/evidence=EXPERIMENTAL
/codon_start=550
mat_peptide 622..1923
/gene="traH"
/evidence=EXPERIMENTAL
/codon_start=622
CDS 1923..4739
/gene="traG"
/codon_start=1923
CDS 4772..>4847
/gene="traS"
/codon_start=4772
BASE COUNT 1289 a 1099 c 1283 g 1176 t
ORIGIN
1 cttttttatc ccgccagccc tgtagaaatt gtcgtttcgc tgagtcttac cttttctgtt
61 tctgttgtgt tttttgtggg ggcaattgtg ggatggattt cggtgtctga aatatattac
121 agccgcatga ccggtctgaa tgaatcatcg gaaatatcag agggaactta taatgagaga
181 aaataaaagt aatcctgaac tcaaaatcag aagtacagaa cgagattata aatacatttc
241 cagaattact ggcaggtatg ctggattgtc tctggtattc ctgactgccg gaattgtttt
301 atggacagtt atggatatta tttttgatgc ctgtattgat tcgtggaaag cggacccgga
361 attaaataat tcgagttata tgtggaatat cctgatatat gccattccgt atacgttata
421 tgcgcttgca gccgggtttc ttgttacatt tttttctgtc cccaatgtac gaattaatat
481 acggaaatat cgagatattc ctgctgaaat gtcgtatgca ccgggagaac acattaaggg
541 agggcaagaa tgatgccacg cattaaacct cttctggttc tttgtgccgc cttactgacg
601 gtgacgcctg cagcgtcagc ggatgtgaac agcgacatga atcagttctt taataagctg
661 ggatttgctt ccaataccac acagccgggc gtctggcagg ggcaggccgc cggctatgct
721 tacggtggct ccctgtatgc ccgtactcag gtcaaaaatg ttcagctaat ttccatgacg
781 ctgccggata tcaatgctgg atgtggcggc atcgacgcct atctcggctc gttcagtttt
841 attaatggtg agcagttgca gcgttttgtt aagcagatta tgagcaatgc tgccggttac
901 ttttttgacc ttgccctgca gacaacggtg ccggaaataa aaaccgcaaa agacttcctg
961 cagaaaatgg caagcgacat taacagtatg aacctcagtt cctgccaggc tgcacagggg
1021 attatcggcg ggcttttccc ccggacgcag gtgtcccagc aaaaagtctg tcaggacatt
1081 gccggtgaga gcaatatttt tgctgactgg gcggcttccc ggcagggatg taccgttggc
1141 gggaaatctg acagcgtcag ggataaagcc agcgacaagg ataaggagcg ggtgaccaaa
1201 aacatcaaca tcatgtggaa tgcgctttcc aaaaacagaa tgtttgacgg caacaaagag
1261 ctgaaagagt ttgtgatgac gctgaccggc tcactggtgt ttggtcccaa cggcgaaatc
1321 acaccgctgt cagccagaac cactgaccgt tcaattatcc gggccatgat ggaaggcggc
1381 acagcaaaaa tttctcactg caacgattct gataaatgcc tgaaagtggt ggcagacaca
1441 ccggtgacca tcagccggga taatgcactg aagtctcaga ttactaaact tctggccagc
1501 attcagaaca aggctgtcag tgacacgcct ctggatgaca aggaaaaagg ctttatttcc
1561 agtaccacca tccccgtctt caaatacctg gttgacccgc agatgctcgg tgtttccaac
1621 agtatgattt accagctgac ggactatatc ggttacgaca tcctgctgca gtacattcag
1681 gagctgatac agcaggcacg ggcgatggtg gccacgggaa attatgacga agcggttatc
1741 gggcatatta acgacaacat gaatgatgcc acccggcaga ttgcggcgtt tcagtcacag
1801 gtgcaggtac agcaggatgc gctgctggtt gtcgatcgtc agatgagcta catgcgtcag
1861 cagctttccg cccgcatgct cagtcgttac cagaacaact atcacttcgg agggagcacg
1921 ctgtgaatga agtttatgtg attgccggtg gtgagtggtt gcggaataac ctgaacgcca
1981 ttgccgcctt tatgggaacc tggacgtggg attccattga aaaaattgcg ctcacattgt
2041 ctgttctcgc ggtggccgta atgtgggtac agcggcacaa cgtgatggat ttgctgggct
2101 gggtggccgt gtttgtgctt atcagcctgc tggttaatgt ccgcacatcg gtgcagatta
2161 ttgataacag tgacctggtc aaagttcacc gggtggataa tgtgccggtc ggtctggcga
2221 tgccactttc actgacgacc cgtatcgggc atgcaatggt ggccagttac gagatgatct
2281 tcacgcaacc ggacagcgtc acctacagca aaacggggat gctgttcggg gcgaatctga
2341 ttgtgaaaag caccgatttc ctgtcccgga atccggaaat catcaatctg ttccaggact
2401 acgtccagaa ctgcgtgctg ggtgatattt acctgaatca taaatacacg ctggaagacc
2461 tgatggcctc cgctgacccc tacacgctga ttttttcccg ccccagcccg ctacggggcg
2521 tttatgacaa caataataat ttcataactt gtaaggatgc gtcggtcacg ctgaaagaca
2581 ggctgaatct cgatacaaag acgggaggca agacctggca ttattatgtg cagcagatat
2641 ttggcggcag accggacccg gacctgttat tcagacaact ggtcagtgac agttacagtt
2701 atttctacgg ctccagtcag tctgccagcc agattatgcg ccagaacgtc accatgaatg
2761 ccctgaaaga gggtatcacc agtaatgcag cccgtaacgg tgacaccgcc agcctggtaa
2821 gtctggccac cacgtcatcg atggagaaac aacgtctggc acatgtctcc attggtcatg
2881 taactatgcg aaacctgccg atggtccaga ctatcctgac ggggattgcc attggtatat
2941 tcccgctcct gatactggcg gctgtcttta acaagctgac attgtctgta ttaaaggggt
3001 atgtgtttgc cctgatgtgg ctgcagacct ggccgttgtt gtatgccatt ctgaacagtg
3061 ccatgacatt ctatgcgaaa cagaatggtg cgccggtcgt gttgtctgaa ctctctcaga
3121 tacagctgaa atactctaac ctggcctcca ctgccgggta tctttcagcc atgatccccc
3181 cgttgtcatg gatgatggta aaaggccttg gcgcaggttt ttccagcgtg tacagccact
3241 tcgcctcttc atctatcagt ccaacggcca gtgcagcagg cagtgtggtt gacggtaatt
3301 actcctacgg caacatgcag acggaaaacg tgaacggctt cagctggagc accaacagca
3361 ccacgtcgtt tggtcagatg atgtaccaga ccggcagtgg cgcaaccgcc acacagaccc
3421 gtgacggtaa tatggtgatg gatgcaagcg gagcgatgtc ccggttaccg gtcggtatca
3481 atgcaacgcg tcagattgca gcggcacaac aggaaatggc ccgggaggcg tcgaacagag
3541 ccgaaagtgc cctgcatggt ttcagcagca gtattgccag tgcctggaac acactcagtc
3601 agtttggctc taaccggggg agcagtgact ctgtcactgg tggtgctgac agcacgatga
3661 gtgctcagga ctccatgatg gccagccgta tgcgcagtgc agtggaaagc tatgcgaagg
3721 cgcataatat cagtaatgag caggcgacga gagaactggc ctcgagaagt acaaatgcgt
3781 cgcttggcct ttacggtgat gcttatgcta aagggcattt aggcataagt gtattgggta
3841 acggtggagg ggttggtctc caggccggag ctaaggcaag cattgatggc agtgatcttg
3901 actcacatga agccagtagt ggttcacggg ccagccatga tgctcgtcat gatatcgatg
3961 ccagagctac acaagacttt aaagaagcca gcgattactt taccagccgc aaagtcagtg
4021 aatccggcag ccatactgac aataatgctg attcccgtgt ggatcagttg tctgcagccc
4081 tgaattcagc gaaacagagt tacgaccagt acacgacgaa tatgacccgc agccatgaat
4141 atgctgaaat ggcatcccgt actgagagta tgagtgggca gatgagtgaa gatctgtcgc
4201 aacagtttgc acagtatgtg atgaaaaacg caccgcagga tgttgaagct attctgacaa
4261 ataccagttc gccggagatt gcagaacgcc gtcgggctat ggcgtggtct tttgtgcagg
4321 aacaggtaca gcctggtgtt gataacacct ggcgtgaatc ccgtagagat ataggtaaag
4381 gaatggagag cgtaccttcg ggtggtggca gccaggatat tattgctgat catcagggac
4441 atcaggccat tattgagcaa agaacgcagg acagtaatat ccgtaatgat gtaaaacatc
4501 aggttgataa tatggtcaca gaatatcgag gtaatatcgg agatactcag aacagcatcc
4561 gaggcgagga aaatattgta aaagggcagt attctgaact acaaaatcat cataaaacag
4621 aagcattaac tcagaataat aaatataatg aagaaaagct ggctcaggaa agaatacctg
4681 gtgctgatag tccgaaagag cttttagaga aagcaaagag ttaccagcat aaagaataat
4741 cataacatgt tgggtagggt atggagagac catgattaca caacaaatta tcagtagtga
4801 gttggaagtt ttaaagaaac atattgactc aggagatatt agaattc
//