GenBank-Updates@genbank.bio.net (03/08/91)
LOCUS CLORUB 2675 bp ds-DNA BCT 08-MAR-1991
DEFINITION C.pasteurianum open reading frame A, B, C, and rubredoxin gene,
complete cds.
ACCESSION M60116
KEYWORDS rubredoxin.
SOURCE C.pasteurianum (strain ATCC 6013) DNA.
ORGANISM Clostridium pasteurianum
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 2675)
AUTHORS Mathieu,I., Meyer,J. and Moulis,J.-M.
JOURNAL Unpublished (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 198..1124
/note="product homologous to E.coli thioredoxin reductase:
J.Biol.Chem. (1988) 263:9015-9019, and to F52a protein of
alkyl hydroperoxide reductase from S.typhimurium:
J.Biol.Chem. (1990) 265:10535-10540"
/note="open reading frame A"
/codon_start=198
CDS 1126..1353
/note="open reading frame B"
/codon_start=1126
CDS 1408..1944
/note="product homologous to the C22 protein component of
alkyl hydroperoxide reductase from S.typhimurium:
J.Biol.Chem (1990) 265:10535-10540"
/note="open reading frame C"
/codon_start=1408
CDS 2157..2321
/note="sequence of product differs from reported protein
sequence (accession number in NBRF-PIR: RUCLEP) in three
positions: D14->N, D22->N, E48->Q"
/product="rubredoxin"
/evidence=EXPERIMENTAL
/codon_start=2157
BASE COUNT 1012 a 275 c 534 g 854 t
ORIGIN
1 aatattggta attttgtaaa aaagcaatgc tgtgcatatt gcatattttt ttttgttata
61 ttataataat taatataagt aaaaggctat attaattaat aatgattatt gtttaatgct
121 gttaatgtaa aaaacatctt tatatattaa aatataaagg tagtctaata tataaattat
181 atggtggtgt ttatgacatg aaagaagaga agcagcttga tttagttata atcggagcag
241 gtcccgcggg acttacggcg gcgatttatg caataagagc taaactcaat actttagttt
301 tagaaaatga acttgtagga ggacaaatta gggaaactta tacagtagaa aatttcccgg
361 gatttaatgt tatatctggt gctgatttgg cagataaaat ggaagagcat gcagcaagca
421 ttggagtaaa tatagatcaa tttagtaata tagaaaaaat taaattatct gatgatgaaa
481 aaattataga aacagaggat gtaatatata aagttaaggc gctgataata gctacaggag
541 ccaaaagcag aagactgcct attccagaag aagaaaaact ccatggaaaa gttattcatt
601 attgtgagtt atgtgatgga gctctatacc aaggaaagga tttggttgtt gtgggcggtg
661 gaaattctgc tgtagaagca gctatattct taactaaata tgctagaaat attactatag
721 ttcatcaatt tgactatctt caagcacaaa agtactcaca ggatgaattg tttaaacata
781 aaaatgtaaa aataatatgg gatagtgaga taagaaatat tgttggagaa aatgagattg
841 aaaaaatagt agtagaaaat gtaaagacaa aacagaaaac agaattgaaa gcagatggag
901 tttttgttta tattggttat gagcctaaga cggaactctt taaagattct attaatataa
961 ataaatgggg atatatagaa accgatgaaa atatggaaac caatataaag ggagtttttg
1021 ctgctggaga tgtaaggtct aaacttataa gacagttaac tacagctgtt agtgatggca
1081 ctgttgctgc actaatggct gaaaaatata taggaggtaa ataatatgat taaaatatat
1141 tccacaccaa catgtccatg gtgtaaaaag acaaaagaat atttaaaatc aaaaaatata
1201 gattttgttg atgtaaatgt agctgatgat atgaaggaaa gagaagaaat gcgtagctta
1261 tccaagcaat caggagtgcc agttataaat atagatggta atataatagt tggatttaac
1321 aaggcagaaa tagataaact aatagagaaa tagactactt tttggtatgg tatatatggt
1381 atatgatgaa caaaaggagg agattttatg gagagattag tgggaaagcc agcaccagaa
1441 tttgaaatga aggctgtcaa aggtgatgga agaggattta ctgaagttaa actgggagat
1501 tataagggaa aatggctagt aatgtttttt tatccgttag attttacatt tgtatgccca
1561 actgaaatta caggctttag caaaagggct gaagaattta gggatttaaa agctgaatta
1621 ttagcggtaa gttgtgatag tcaatattct catgagactt ggattaatca agatataaaa
1681 caggggggac ttggtaaaat taattttcca attgcctccg ataaaactac tgaagtgagt
1741 acaaaatatg gaatacagat agaagaagaa ggtatttctc taagaggact atttataatt
1801 gatcctgaag gaatagttag gtattctgta gtgcatgatc ttaatgtagg aagaagtgtt
1861 gatgaaactc ttcgtgtatt aaaggcattt caaactggtg gaatgtgtgc tttagattgg
1921 catgaaggag atgacaactt ataatttatg gggatttgat cttatatgac tagactaaat
1981 tttattatta aaacttgaaa tatatactat atatttcaag ttttaattga agttcatatt
2041 gcataaattt ttgttaaata agtatatttt tataaaataa ggtagaaata tttacttaat
2101 tgttgtaata taaaataaat acattaaaat tttattaagg tattggagga gtttttatga
2161 agaagtatac atgtacagta tgtggatata tttataatcc cgaagatgga gacccagata
2221 atggagtaaa tccaggaaca gattttaaag atattccaga tgattgggta tgtcctttgt
2281 gtggagtagg aaaagatcaa tttgaagaag tagaagaata ggataaatta aagactggga
2341 gagaatttgc ttccagtctt taattatgat tcactatatt tgtatatcta cattatataa
2401 tcttagccaa gtatctactt gaataagata agctattaat tgaggtcctg tcattaattg
2461 accataccaa ggttttttat aggatttacc tccagttctc acaatctctc ttactacagg
2521 aacatcgatc aattgaagta taggagaatt aggatcattt aaaatatttt caagccattt
2581 ttgtactatt ttagtatatc caggattaaa ggttttagga taaggactct tttttctata
2641 gacaatatca tcaggaagta ttccctttaa agctt
//