GenBank-Updates@genbank.bio.net (03/09/91)
LOCUS ECOPRIAY 2907 bp ds-DNA BCT 09-MAR-1991
DEFINITION E.coli primosomal replication factor Y (priA) gene, complete cds.
ACCESSION D00616 M33881
KEYWORDS priA gene; replication factor Y.
SOURCE Escherichia coli (strain K-12; cell line HMS-83) genomic DNA, clone
Y-20.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 2907)
AUTHORS Nurse,P., DiGate,R.J., Zavitz,K.H. and Marians,K.J.
TITLE Molecular cloning and DNA sequence analysis of Escherichia coli
priA, the gene encoding the primosomal protein relication factor Y
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 87, 4615-4619 (1990)
STANDARD full staff_entry
REFERENCE 2 (sites)
AUTHORS Lee,E.H., Masai,H., Allen,G.C. and Kornberg,A.
TITLE The priA gene encoding the primosomal replicative n' protein of
Escherichia coli
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 87, 4620-4624 (1990)
STANDARD full staff_review
COMMENT
[Proc. Natl. Acad. Sci. U.S.A. 87, 4620-4624 (1990)] sites.
These data kindly submitted in computer readable form by:
Kenneth J. Marians
Rockefeller Research Laboratory
Memorial Sloan-Kettering Cancer Center
430 East 67th Street
New York NY 10021
U. S. A.
Phone: 212-639-5890
Fax: 212-717-3627
FEATURES Location/Qualifiers
misc_signal 354..359
/note="-35 box"
misc_signal 369..374
/note="-10 box"
misc_binding 395..400
/note="pot. ribosome binding site"
CDS 406..2604
/note="primosomal protein replication factor Y"
BASE COUNT 649 a 795 c 816 g 647 t
ORIGIN Map position 88.5 min.
1 cccgggatgt tgaaacgctt gttgaagcgg tcaacacggc caccggtagc aacatcacgc
61 tgtttgccag tgaagaacgg gtggcacttg ctgcacacgt cgaggttcag gtcatgacca
121 acggtggagc ggattttcat tacgttaccg caagagcagc tagcagtaat ttcttcgtat
181 ttcgggtgaa tatctttttt catgggaaaa cctcggttta aggccgcgtc gctcttccag
241 ccctaacgcc agacaccacg cgatgttaaa agtatagctt caatacgatc atttcgtacg
301 aaggcgcgaa atcatacaga aattaaccag cgtatgcaaa ctgatccgca ctcttctacg
361 gcaatgtgta tactaaccca ccgaatttca agtcaggatg atgctatgcc cgttgcccac
421 gttgccttgc ccgttccgct tcctcgtacc tttgactatc tgctgccaga aggcatgacg
481 gttaaagctg ggtgtcgcgt gcgcgtgccg tttggcaaac agcaggagcg catcgggatt
541 gtggtatcag ttagcgatgc cagcgaactg ccgctcaatg agctaaaagc ggtagtcgaa
601 gtgctggata gtgagccggt gtttactcac tccgtctggc gattgctgct atgggcggca
661 gattactatc atcatccgat tggcgatgtg ctgtttcatg ccttgccgat tttactacgc
721 caggggcggc ctgcggcgaa cgcgccgatg tggtactggt ttgccactga acaaggccag
781 gcggtggatc tgaacagcct gaaacgctcc cccaagcaac aacaggcgct ggcggcgtta
841 cggcaaggca aaatctggcg cgaccaggtc gccacgctcg aatttaatga tgccgcgttg
901 caggcgctac gcaaaaaagg tctgtgtgat ttagcaagtg aaacaccaga gtttagcgac
961 tggcgaacga actatgccgt ttctggtgag cggttgcgat tgaataccga acaggccacc
1021 gccgttggcg caattcatag cgcggcagat actttttctg cctggctgct ggcgggcgtt
1081 accggttccg gtaaaacgga ggtttatctc agcgtactgg aaaacgtgct cgctcagggc
1141 aaacaggcgc tggtgatggt gccggaaatc ggcctgacac cgcaaactat cgcccgtttt
1201 cgtgaacgtt ttaatgcccc cgtggaagtt ctgcattccg gcctgaacga cagcgagcgt
1261 ctttcggcgt ggctgaaagc gaaaaatggt gaggcggcga ttgtgatcgg cacccgctcc
1321 gcgctgttta cgccgtttaa aaatctcggc gtgattgtca ttgatgaaga gcacgacagc
1381 tcctacaagc agcaggaagg ctggcgctat catgcccgcg acctggcggt gtatcgtgcg
1441 cacagcgagc aaatcccgat tattcttggc tccgcaacgc ccgcgctgga aacgttatgc
1501 aacgtccagc agaaaaaata ccgcctgctg cgcctgaccc gtcgggcagg gaatgcgcgt
1561 ccggcaattc aacatgtgct ggatttaaaa ggtcagaagg tgcaggcagg tctggctccg
1621 gcgttaatca ctcgtatgcg ccagcattta caggctgata accaggtcat tctctttctt
1681 aaccgccgtg gctttgcgcc tgcactgctg tgccacgact gtggctggat tgccgaatgc
1741 ccacgttgcg atcactacta cacgctgcat caggcgcagc accatctgcg ctgccaccac
1801 tgtgacagtc agcgtccggt gccgcgccag tgcccttcct gcggttccac gcacctggtc
1861 cccgtggggc tgggcaccga acagcttgaa cagacgctcg cgccgttgtt ccccggcgtg
1921 cccatttctc gtatcgaccg cgataccacc agccgcaaag gggcgctgga acagcaactg
1981 gcagaagtac atcgcggcgg cgcgcggatt ttgattggta cacaaatgct ggcgaaaggt
2041 caccatttcc cggatgtgac gctggttgca ttactggacg tggacggcgc gctgttttct
2101 gccgattttc gctcggcaga gcgtttcgct cagctttaca cccaggtcgc cggtcgtgcc
2161 gggcgtgcgg gtaaacaggg cgaagtggtg ctgcaaacgc accatccgga acatcctctg
2221 ttgcaaacgt tgctctataa aggctacgac gcctttgccg aacaggcgct ggctgagcgg
2281 cgaatgatgc agctaccgcc gtggaccagc catgtgattg tgcgtgcgga agatcataac
2341 aatcagcacg tgccattgtt cctgcaacaa ctgcgtaatc tgatcctctc cagcccactg
2401 gcagacgaga aactgtgggt tctcggtccg gttccggctc tggcacctaa acgtggcggt
2461 cgctggcgct ggcagatatt gttgcagcac ccttcccgcg tgcgcttgca acacatcatt
2521 aacggtacgc tggcgctcat caatacaata ccggattccc gtaaggtgaa atgggtgctg
2581 gatgttgatc cgattgaggg ttaaaccgct cacgatgcga ggcggatcga aaaattcaat
2641 attcatcaca cttttcatga aaattctgta accgttttca cgcgctatct gctaaaaatg
2701 ttgccgatgt gaagtaaaca tggatgtagt acgcctgacg tgccaggcga ggagtgagtg
2761 tgaaagcgaa gaagcaggaa actgccgcga ccatgaaaga cgttgccctc aaggcaaaag
2821 tctctacagc gaccgtctcc cgagcattaa tgaatcccga taaagtctcc caggccaccc
2881 gtaatcgggt tgaaaaagcg gcccggg
//