GenBank-Updates@genbank.bio.net (03/13/91)
LOCUS LCALACE 2100 bp ds-DNA BCT 13-MAR-1991
DEFINITION L.casei phosphotransferase system lactose-specific enzyme II (lacE)
gene, complete cds.
ACCESSION M60851
KEYWORDS lactose-specific enzyme II;
phosphoenolpyruvate-dependent phosphotransferase system.
SOURCE L.casei DNA.
ORGANISM Lactobacillus casei
Prokaryota; Bacteria; Firmicutes; Regular asporogenous rods;
Lactobacillaceae.
REFERENCE 1 (bases 1 to 2100)
AUTHORS Alpert,C.-A. and Chassy,B.M.
TITLE Molecular cloning and DNA sequence of lacE, the gene encoding the
lactose-specific enzyme II of the phosphotransferase system of
Lactobacillus casei: Evidence that a cysteine residue is essential
for sugar phosphorylation
JOURNAL J. Biol. Chem. 265, 22561-22568 (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS <1..228
/label=URF
/codon_start=1
CDS 349..2082
/product="phosphotransferase system lactose-specific
enzyme II"
/gene="lacE"
/codon_start=349
BASE COUNT 536 a 470 c 486 g 608 t
ORIGIN
1 ctgcagcgtg ttgatacata tgccgttacc attgtccctg aggttgccac tgaacttaag
61 caaaactatc cgcagtctta caagattgcc tcagaaattt tcgatgaaat taaggatcaa
121 ctctatcgca gtatgagtga ggacgaacga ctttacttca tcatccacat tcagcgattg
181 ataaacgaag caccagccca gaatcattca caaaacgatt cattataacg cgctcgcagt
241 cgtagaagcc tacacataag ggctttgaag caatctacca aagattgggc cagtttgctt
301 caagcacgct tatactttgg cttccaagcg ctcaggagga aaagactcat gaataaggtt
361 tttgataaat taaaaccggt ttttgaagcc atcgctgcta acaaatatat ttccgcgatt
421 cgtgatggct ttatcgcatg tatgccgatc atcatcttct caagtatctt tatgatggtt
481 gcttatgttc cgaacgcttg ggggttttac tggccagaca acgtaacgaa tacgttgatg
541 gttgcttata actattccat gggcttgctg gcactgtttg tggcgggaac gactgctaaa
601 aacttgactg acagcaagaa ccttgaatta ccgaaaacaa atcaaattaa tccagtggcc
661 gtcattgtgg cgtctgaaat ttcttttgtc attttgtcga tcttgccgct taaaaccggt
721 gttgacctga cttacatggg gactcaggga ttgatctgcg cttatatcgt tgggttgatc
781 gtgccaaata tttactacgt ctgtattaag aacaatgtca cgattaaatt accagagcag
841 gtaccaggta atatcgccca gtcatttaaa gaccttatcc cgatgggcct ttctgtgaca
901 gccttctggc ttttcggtgt tggcttcaaa gcggcaacgg gtactgtatt gccacgctgg
961 atcattcaag tactttcgcc gcttttccaa gccagcgatt cttatctagg cttagccctg
1021 attgctggcg cgatggcttt cttctggttc tgtggtgtcc aaggcccatc aattgttcag
1081 ccggctgttg tcccgattat gattgcgaat acagcagcta acttgcagca atatcaggca
1141 gggcaacatg tctcgcacgt tttggccatg aacacgatgg actacgtcat gaattttggt
1201 ggaaccggag caacattggt ggtacctttc atcatgctgt ttgcggcccg atcagcgcaa
1261 ttaaaagccg ttggtaaggc tgcctttgtg ccatgcactt tcggggtcaa tgaacctgtt
1321 ttgttcggaa tgccgattat catgaatccc atgttattca ttccgttctt agcaaccccg
1381 atcgtgaacg tttgcttatt caaattcttt gtgagtgtac ttggcatgaa cagtatgatg
1441 tacaccatgc catggacagt tccgggaccg attgggattt tgatttcgac tgggtttgcc
1501 ccgttggctt tcgtctttgt tctactgacg ttggttctgg acgttgcgat atacttccca
1561 ttcattcgcg tttatgacag cacactctta gctgaagaaa aagctaagga agaggtcatt
1621 gaagacgacg gcatggctgt tctggcgagt gacacagtgt caccttctat cccgactggt
1681 ttgactgttg cgactgccac agacgacgat gcgacccatg tgttgcctga aacagcccca
1741 tctgcccacg gtgaagccta tttcaaacaa aatgaagttg atgttttagt gttgtgtgct
1801 ggtggtggaa caagcggtat tcttgccaac gcgctaaaca aactgtctaa ggaacgcggt
1861 ttaaagctct cggccgccgc ccgcgcttat ggacaggata tggatttgat caaagatatg
1921 aacatggtca tccttgcgcc gcaaatggaa agcatgaaag gtaatttaaa aaagatcacc
1981 gataagtatg gcgtcaagct ggtcacaaca actggtcggc aatacattga attaaccaac
2041 aatggtgaca tggcacttga ttttgttgaa tccaatcttt gagacactta acaggaggtt
//