GenBank-Updates@genbank.bio.net (03/13/91)
LOCUS ACAMYSHC 6756 bp ds-DNA INV 13-MAR-1991
DEFINITION Acanthamoeba castellanii myosin heavy chain (HMWMI) gene, complete
cds.
ACCESSION M60954 J05678
KEYWORDS myosin heavy chain.
SOURCE Acanthamoeba castellanii vegetative state DNA.
ORGANISM Acanthamoeba castellanii
Eukaryota; Animalia; Protozoa; Sarcomastigophora; Sarcodina;
Rhizopoda; Lobosa; Gymnamoeba; Amoebida; Acanthopodina;
Acanthamoebidae.
REFERENCE 1 (bases 1 to 6756)
AUTHORS Horowitz,J.A. and Hammer,J.A.III.
TITLE A new Acanthamoeba myosin heavy chain: Cloning of the gene and
immunological identification of the polypeptide
JOURNAL J. Biol. Chem. 265, 20646-20652 (1990)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS join(278..439,566..718,875..1045,1133..1274,1346..1688,
1745..2021,2079..2559,2634..2835,2938..3197,3271..4191,
4290..4445,4527..4759,4847..4953,5035..5310,5394..5640,
5788..5874,5962..6356,6453..6573)
/product="myosin heavy chain"
/gene="HMWMI"
/codon_start=278
intron 440..565
/number=1
/gene="HMWMI"
exon 566..718
/number=2
/gene="HMWMI"
intron 719..874
/number=2
/gene="HMWMI"
exon 875..1045
/number=3
/gene="HMWMI"
intron 1046..1132
/number=3
/gene="HMWMI"
exon 1133..1274
/number=4
/gene="HMWMI"
intron 1275..1345
/number=4
/gene="HMWMI"
exon 1346..1688
/number=5
/gene="HMWMI"
intron 1689..1744
/number=5
/gene="HMWMI"
exon 1745..2021
/number=6
/gene="HMWMI"
intron 2022..2078
/number=6
/gene="HMWMI"
exon 2079..2559
/number=7
/gene="HMWMI"
intron 2560..2633
/number=7
/gene="HMWMI"
exon 2634..2835
/number=8
/gene="HMWMI"
intron 2836..2937
/number=8
/gene="HMWMI"
exon 2938..3197
/number=9
/gene="HMWMI"
intron 3198..3270
/number=9
/gene="HMWMI"
exon 3271..4191
/number=10
/gene="HMWMI"
intron 4192..4289
/number=10
/gene="HMWMI"
exon 4290..4445
/number=11
/gene="HMWMI"
intron 4446..4526
/number=11
/gene="HMWMI"
exon 4527..4759
/number=12
/gene="HMWMI"
intron 4760..4846
/number=12
/gene="HMWMI"
exon 4847..4953
/number=13
/gene="HMWMI"
intron 4954..5034
/number=13
/gene="HMWMI"
exon 5035..5310
/number=14
/gene="HMWMI"
intron 5311..5393
/number=14
/gene="HMWMI"
exon 5394..5640
/number=15
/gene="HMWMI"
intron 5641..5787
/number=15
/gene="HMWMI"
exon 5788..5874
/number=16
/gene="HMWMI"
intron 5875..5961
/number=16
/gene="HMWMI"
exon 5962..6356
/number=17
/gene="HMWMI"
intron 6357..6452
/number=17
/gene="HMWMI"
BASE COUNT 1446 a 2080 c 1871 g 1357 t 2 others
ORIGIN
1 tttgagagca cacaagatct tttcctgtac actaggacac atagagccca cccggcaacc
61 gaaccaaaat caagcgcagc tcataagatg gcgtcagtag atggttggaa gcagtacttc
121 actgctgagg gcaacgccta ctactacaat gaagtgtcgg gagagacatc ttgggaccct
181 cccagctctt tgcagagcca tgattacaac gtaagcctcc cttcttccag ctcccttccc
241 ctcatccccg ttgcctgccc aagtcgagcg cattctcatg cattttttgt ttttttttgc
301 taatcgccaa tttctatcat taaaatgccc tcaggctgag ttcgtgtgga ttccccaccc
361 tgtccatggc tacatcacag gcaagttcat tcaagaggac tacggtggca cctcctactg
421 ccagaccgaa gagggcgagg tgaggagccc ttgattcatt ttgagtagcg cggattatca
481 caacgttgat tacggctctc tgctttttgg ttgtgctcgc tcactcactc atggcctttg
541 cgtggtgtca tgcacaacac accagtcctt gagcgtggcg tgtgcgccct cacagctggc
601 caaggtcgcc aagtctgtac tggacaagag tgtggacgat ctcgtgcaaa tggacgacat
661 taacgaggct atgatcgtcc acaacttgag gaagaggttc aagaacgatc aaatctatgt
721 acgcctcccc ttcatcggtt accgggcttc ttttgcgcga tgatgtcatt ctatttcaaa
781 tattaattaa tttctcataa attaaattat attaaattta ttattttctt ttttgttgat
841 ttttaatcga ttgccttcct ctcgcggtgt gcagaccaac attggtacga tcttgatctc
901 tgtgaatccc ttcaagcgtc tgccgctgta cacccccacg gtgatggacc agtacatgca
961 caaggtaccg aaggaaatgc ctccgcacac ctacaacatc gccgatgacg cctacagggc
1021 catgatcgac aatcgtatga atcaggtgca taccacacca cttcgcctgc ctggctctgc
1081 gcgtgaagct cagatgatcg aatgttgaca acgatgcgtg tatggcgcgc agtccatctt
1141 gatctccggc gagtctggcg ctggtaagac cgagtgcacc aagcagtgct tgacctactt
1201 cgccgagttg gctggctcga ccaatggagt ggaacagaac attctgctcg ccaatcccat
1261 tctcgaatcg ttcggtatgc gtcatcacat tccacattct atcttcgtac atggcctcat
1321 gccacctctt cttcacatcg atcaggtaac gccaaaacgc tgcgcaacaa ctcctcgcgt
1381 ttcggtaagt gggtggagat ccacttcgat cagaagggct ccatctgcgg tgcctcgacg
1441 atcaatcatc tgctcgagaa gtcgcgcgtc gtgtaccaga tcaagggcga gcgtaacttc
1501 cggatagttg ctaccgagct ggtcaaggcg ccaccgcgga gcagaggcgg aggtggttcg
1561 tctccggccc gcccggaatc cttcaagttc ctcagccagt cgggctgcat tgacgtcgag
1621 ggagtcgacg acgtcaagga attcgaggaa cgtgttcttt gccatgggca agctcgggta
1681 cgcgtgcagt cctcatcact tcaatcatct cgacttgcta accaacactt tgtgtacttg
1741 acaggttctc ggaagacgac atcaacaact gcatggagct gatctcggcc atcttgcact
1801 tgggcaactt cgagttcgtg tcgggccagg gcaagaacgt ggagacctcc accgtggcca
1861 acagggagga ggtgaagatc gtcgcgacgc tgctcaaggt ggatccggcc accctggagc
1921 agaacgtgac cagcaagctg atggagatca agggctgcga tcccacccgc attcctctca
1981 cgcccgtcca ggccaccgac gcgaccaacg ccctggccaa ggtaagcgca acgagcggag
2041 gcaacgcaga gctgattgct aacacacttt ctccacaggc catctattcc aagctgttcg
2101 attggctcgt gaagaagatt aacgagtcga tggagccgca aaagggcgcc aagaccacca
2161 ccatcggtgt gctcgacatc ttcggtttcg agatcttcga caagaacagc ttcgagcagc
2221 tctgcatcaa cttcaccaac gagaagctgc agcagcactt caaccagtac acgttcaagc
2281 tcgaggagaa gctgtaccag tccgaggagg tcaaatacga gcacatcacc ttcatcgaca
2341 accagccggt gctggacctg atcgagaaga agcagccgca gggcctcatg ctcgtcctcg
2401 atgagcagat ctccatcccc aagtccagcg acgccacctt cttcatcaag gccaaccaga
2461 cccaggcggc aagaagcacg caactacgag gaggtgagga cagcaggacc gacttcatca
2521 tcaagcacta cgccggtgac gtcatctacg actccacagg tacatcttcc ctcccacatt
2581 cttgctgcta gcacaccagc agctaacaag gctcgtcgat atcgcgtcgc caggcatgct
2641 cgagaagaac aaggacaccc tgcagaagga tctgctggtc ctttcggaga gcagcaagca
2701 gaagctgatg aagctcctct tcccgccctc ggagggcgac cagaagacct ccaaggtgac
2761 cctcggtggt cagttcagga agcaactcga cagcctcatg accgccctca acgccaccga
2821 gccccactac atcaggtatg ctcttctcca tgcgtgtgtc gcgtgtcgct gtcgtgtcgt
2881 gtcttcagcc ttgtttgtgt gtcgtgtgct aatgcattag ctatgtcgtc gccacaggtg
2941 catcaagccc aactcggaga agcaggccga tctcttccac ggcttcatgt cgctgcagca
3001 gctccgctac gccggtgtgt tcgaggccgt ccgcatccgt cagaccggtt acccgttccg
3061 ctactcgcac gagaacttcc tcaagcggta tggtttcctc gtcaaggaca tccacaagag
3121 atacggcccc aacctgaagc agaactgcga cctcctcctc aagagcatga agggcgactg
3181 thtgtccaag gttcagggta cgaacgtcgg ggtgtgtgtg tgtgtgtgtg tgtggtgttg
3241 gaaactcact ggggtgcggt tggtgtgcag tgggtaagac gagggtgctg tacagagcgc
3301 cggagcagcg gggtctcgag ctgcagcgca acattgcggt ggagcgagtc accatccaga
3361 ttcaggccgg cgtgcggcgg atgttcgcca ggcgccttta caagaggatg cgcgccatca
3421 agccggtgct gctcaacgcc atcaagtcgc gctcgctcag cgtgctggag caggccatcg
3481 atgccgccaa ggacatcgag ttcgacatga agctcatccg cgactgcaag gagctcagga
3541 gcgtcatctt gaaggagatg gaaatcacca agaagctcac tgactacatc ggagccccgc
3601 ccaaccacaa gacctacctg caagtggagc cgctctacgc ccagctgtgc gcggtgctca
3661 ccgaagccga gagcatcaac tactcgaccc cgctcgtgga gaccggtcag cagatcaagt
3721 acatgatcgc ccagcgcgtt gagaccaggg agcagctcaa gcaggccgtg gatggcgccg
3781 tgcgcgtcga cctcgaggcg gcgatcgcga gggccgagca gatcggactc gaggagagcg
3841 agccgacgct ggcggccggc cggcaggagc tgcagcgtat ctacagagag gaggagctgg
3901 tggcggagct cctcaacgcg ctggccgtcg gcatggccat gcgcacgagc gagacgacgt
3961 gggaccacgc cgcgatcgac gcccacacgc tcgggtcggc catctacgcc gccgagtcct
4021 tcggcttccg caccgagcag ggccggctca cccttgacga ggccaaggtc atcgtcgagg
4081 tgcgacagca cctggccgcc gacgactacg agtcgctgtc catgaccctg aagaaggcca
4141 ccaccaccct caacaagaac agcatgtccc agtcgaccaa gaccgagatc ggtacgcacc
4201 cctcgcccgc ctttggcacg cacgcacgca cgccgagcgt atgtttcaac ttcggctgtg
4261 ctgaccggtc gtgcggtgtc gtcgtacaga cgaggcctac gaggagctgt cgcacaacac
4321 ggccgtgaac gacctgatcg agaaggtggt gcaggccatc caggaccacg accaggagat
4381 gctcgactac ggcgtccagc aggctgactc cctgcgcatc accgaccgac cagagatgat
4441 gcagggtacc gccacccccg cacgactacg acaacgatga ccatgacaat gacaagaatg
4501 ctgacggtgg tgtgttgctg ttgcagctac cgagctgttg aacaggatcg tcaacgcgcg
4561 cgccctgctg cgcgagggta tcaccaacgt cgaccaggcc cagctggaga cggccctcgc
4621 cgacgccgcc tccttcgcct acacccgcga ggaggtgccc accgcccagc agcttctgga
4681 ccgcatctac gtcatcaacc acgacgccga tgtcggtctc tactacatgg agaaggagcc
4741 cctcgagcgc gcctgggcgg tacttataca tttcactctc tatcatcgct gttgttgttg
4801 ttgttttgtg gttgttgact ggtgattgct cggggcggtt gaacagggcg cgcaagagat
4861 caacctcaag accgcgcaga tcgacgaggt caggaacgtt ctggccaacg acgagcagaa
4921 gttcatccag gagcagctca agactgccaa caggtaatac aatcggttcg gtcgacgcca
4981 tcgatgtggc gcccatgaaa ctcacgacag ttcgttcaac ttgcttggct gcaggctggg
5041 cgatcaggcc agggccattc gactcaacat caagctcaag gagatcttct tcggccagtt
5101 cggcaagatg ttcgtcttcg agcagttcgg cggcctgcgc aagcccgagg actttgccaa
5161 ggccaagctc ctgggcaggg aggccctcaa gctgggcatg ttcaagtgga ccaagagccc
5221 gatccccacc tcgctcacca ccctggacca gctcgccgtc aagagcgcca cccgcctctt
5281 caagaacgtg ctcggcttca tgggcgacag gtacacatca aacccaatcc agcagccgca
5341 acaccgcttc agcttcaatg cgaactcact cgcgcgcgcg tctgtgttac taggccgctg
5401 ccgtacccga atgcgctggc gcaggacctg ctggagcagt gcctggcggc gccggagctc
5461 aggaacgagg tgtactgcca gatcatcaag cagctgaccg agaacccgtc gccgcagtcg
5521 gtgaccaagg gctggcagct catgcggtgc tgccttcaga ccttcccgcc gtcggaggag
5581 ttcgccaact gcctcgagat gttcctcgcc gccaagggca aggacgacaa gtacatcgag
5641 gtaacccgct cgaccttctc tctctgtgtg tgtgcgtgcg tgcgtgcgtg cgtgcgtgcg
5701 tgcgtgcgtg cgtgcgtgcg tgcggcgtgt gcggcgtgtg cgtaacactg tgtgcgcacg
5761 agtactcatt ccttgttctc tcttcagatg ttgcacgaca cgcagtatgg cgacaagcgc
5821 acgtcggcgc ctaatgtgga gcagatcctc gccgccaagc aatatgtgac tcgggtacgc
5881 cattctcgac acccgcgcgc gcatgtgctc aagcgattgt aatgtactga cgttcgattc
5941 cgacaccacc accaccaaca gatcgacctc aaccagtcga cccaggtcga cacgtcggtg
6001 gtgccgcagg gcttcgtgcc cgagaagctg atcgccgacg acacggaggg cgtgatccgt
6061 gcgggttccc gtccggcgca ggcgcgcgct cagcccggcc agcaggctca gcccgccggt
6121 gcggcccggc aacaggccgc cgcgcccgtg caggccgctg cggctaccgc ctcctacgac
6181 tacggccagc agcagcaaca gcagcagcag ggctacgacc agcagcagca ggcctatggc
6241 ggcggcgccg actacggcca gcagcagcag cagcaggacc tgccggccga gccgaccgaa
6301 gagtacaagc aggtggaggt cgtgtacgac tacgatggcg gcggagacgc ccagcggtac
6361 cgccagcggt acgcgcacat tcttctcttc tcttctcttc tttgcttttg ctgatgtctt
6421 gtcthtttgt ctgtttgttt gtccggccat aggttggtgc tggtgaaggg cgccatcatc
6481 accgtcatca aggagtacga gggctgggcc tatggttcga cggatgacgg ccaggtgggc
6541 ctctacccga tcaactacac ccgacccatc taaaggctct gcgcgcccaa cgtcccgcgg
6601 tggctggcca caaccccctt ctctctccct ccctcaacag ttttgtcccg gcttctgggt
6661 tcgttgggcc agcccaagcc cagaacccct tttccggttt tctctccatc gtatcatatt
6721 caccgcttgt ttggcaaata cacgccagca ctttgt
//