GenBank-Updates@genbank.bio.net (03/13/91)
LOCUS POIRNA3 1367 bp ss-mRNA VRL 13-MAR-1991
DEFINITION Porcine respiratory coronavirus mRNA 3, 5' end.
ACCESSION D00658
KEYWORDS peplomer gene; subgenomic mRNA.
SOURCE Porcine respiratory coronavirus (strain 86/137004, 86/135308),
British Isolates, cDNA to mRNA, from viral infected LLC-PK1 cell
lines.
ORGANISM Porcine respiratory coronavirus
Viridae; ss-RNA enveloped viruses; Positive strand RNA virus;
Coronaviridae.
REFERENCE 1 (bases 1 to 1367)
AUTHORS Page,K.W., Mawditt,K.L. and Britton,P.
TITLE Sequence comparison of the 5'-end of mRNA 3 from transmissible
gastroenteritis virus and porcine respiratory coronavirus
JOURNAL Unpublished (1990)
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Paul Britton
Division of Molecular Biology
Agricultural and Food Research Council
Institute for Animal Health
Compton Laboratory
Compton, Newbury
Berkshire, RG16 ONN
U.K.
Phone: +44-635-578411 x211
Email: britton@irad.afrc.ac.uk
Fax: +44-635-578844
It is reported that the nucleotide sequences (474-480 and 1168-
1173) potentially involve in the subgenomic mRNA synthesis
in [J. Gen. Virol. (1990) In press].
FEATURES Location/Qualifiers
CDS <1..185
/note="peplomer gene (AA at 3)"
/codon_start=3
variation 282..282
/note="g in strain 86/137004; t in strain 86/135308"
CDS 490..1224
/note="ORF-3"
/codon_start=490
misc_feature 553..555
/note="pot. N-glycosylation site"
misc_feature 883..885
/note="pot. N-glycosylation site"
CDS 1211..>1367
/note="ORF-4"
/codon_start=1211
BASE COUNT 413 a 215 c 261 g 478 t
ORIGIN
1 ggccttggta tgtgtggcta ctaataggct tagtagtaat attttgcata ccattactgc
61 tattttgctg ttgtagtaca ggttgctgtg gatgcatagg ttgtttagga agttgttgtc
121 actctatatt cagtagaaga caatttgaaa attatgaacc tattgaaaaa gtgcacgtcc
181 attaaattta aaatgttaat tttatctgct ataatatcat ttgttgttaa ggatgatgaa
241 taaagaactt tcaagtcagt caaatttact aatacatccg tggacgttgt acttgacgaa
301 cttgattgtg tatactttgc tgtaaccctt aaagtagaat ttaagacttg taaattactt
361 gtgtgcatag gttttggtga catacttctt gcggctattg aagaagtaaa cacacaaaat
421 ccaaagcatt aagtgttaca aaacaattaa agagagacta tagaaaaact cgaactaaac
481 tttgtgaaaa tgattggtgg actttttctt aatactctga gttttgtaat tgttagtaac
541 catcctatcg ttaataacac agcaaatgtg catcatatac aacaagaacg tgttatagta
601 caacagcacc atgttgttag tgctagaaca caaaactatt acccagagtt cagcatcgct
661 gtactttttg tatcttttct agctttgtac cgtagtacaa actttaagac gtgtgtcggc
721 atcttaatgt ttaagatttt atcaatgaca cttttaggac ctatgcttat agcatacggt
781 tactacattg atggcattgt tacaacaact gtcttatctt taagattcgc ctacttagca
841 tacttttggt atgttaatag taggtttgaa tttattttat acaatacaac gacactcatg
901 tttgtacatg gcagagctgc accgtttaag agaagttctc acagctctat ttatgtcaca
961 ttgtatggtg gcataaatta tatgtttgtg aatgacctca cgttgcattt tgtagaccct
1021 atgcttgtaa gcatagcaat acgtggctta gctcatgctg atctaactgt agttagagca
1081 gttgaacttc tcaatggtga ttttatttat gtattttcac aggagcccgt agtcggtgtt
1141 tacaatgcag ccttttctca ggcggttcta aacgaaattg acttaaaaga agaagaggga
1201 gaccgtacct atgacgtttc ctagggcatt gactgtcata gatgacaacg gaatggtcat
1261 tagcatcatt ttttggttcc tgttgataat tatattgata ttattttcaa tagcattgct
1321 aaatataatt aagctatgca tggtgtgttg caatttagga aggacag
//