GenBank-Updates@genbank.bio.net (03/13/91)
LOCUS TSWSRS 2916 bp ss-RNA VRL 13-MAR-1991
DEFINITION Tomato spotted wilt virus S RNA segment, complete sequence.
ACCESSION D00645
KEYWORDS non-structural protein; nucleocapsid protein.
SOURCE Tomato spotted wilt virus (isolate CPNH9), cDNA to viral RNA.
ORGANISM Tomato spotted wilt virus
Viridae; ss-RNA enveloped viruses; Negative strand RNA viruses;
Bunyaviridae.
REFERENCE 1 (bases 2 to 72; 2848 to 2915)
AUTHORS De Haan,P., Wagemakers,L., Peters,D. and Goldbach,R.
TITLE Molecular cloning and terminal sequence determination of the S and
M RNAs of tomato spotted wilt virus
JOURNAL J. Gen. Virol. 70, 3469-3473 (1989)
STANDARD full staff_entry
REFERENCE 2 (bases 1 to 2916)
AUTHORS De Haan,P., Wagemakers,L., Peters,D. and Goldbach,R.
TITLE The S RNA segment of tomato spotted wilt virus has an ambisense
character
JOURNAL J. Gen. Virol. 71, 1001-1007 (1990)
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Peter De Haan
Department of Virology
Agricultural University Wageningen
Binnenhaven 11
6709 PD Wageningen
The Netherlands
Phone: 08370-83094
Email: molvir@rcl.wan.nl
Fax: 08370-84820
FEATURES Location/Qualifiers
repeat_region 1..70
/note="inverted complementary sequence"
variation 16..16
/note="g in [J. Gen. Virol. 71, 1001-1007 (1990)]; c in
[J. Gen. Virol. 70, 3469-3473 (1989)]"
CDS 89..1483
/note="non-structural protein"
misc_feature 483..491
/note="pot. N-glycosylation site"
misc_feature 716..724
/note="pot. N-glycosylation site"
misc_feature 896..904
/note="pot. N-glycosylation site"
misc_feature 959..967
/note="pot. N-glycosylation site"
misc_feature 1229..1237
/note="pot. N-glycosylation site"
repeat_region 1583..1835
/note="hairpin structure"
CDS complement(1987..2763)
/note="nucleocapsid protein"
repeat_region 2850..2916
/note="inverted complementary sequence"
BASE COUNT 922 a 563 c 468 g 963 t
ORIGIN 5' end of S RNA segment.
1 agagcaattg tgtcagaatt ttgttcataa tcaaacctca cttagaaaat cacaatactg
61 taataagaac acagtaccaa taaccataat gtcttcaagt gtttatgagt cgatcattca
121 gacaagagct tcagtctggg gatcaactgc atctggtaaa gctgttgtag attcttactg
181 gattcatgaa cttggtactg gttctcaact agttcagacc cagctgtatt ctgattcaag
241 aagcaaagta gtcctttggc tatactgcaa agtagggatc ttccctgtga agaagaagag
301 atttctttct cagcatgtgt atatccctat ttttgatgat attgatttta gcatcaatat
361 tgataactct gttctggcac tatctgtttg ctcaaataca gtcaatgcta acggagtgaa
421 acatcaaggt catttgaagg ttttgtctcc tgcccagctc cactctattg aatctatcat
481 gaacagatct gatattacag accgattcca gctccaagaa aaagacataa ttcccaatga
541 caaatacatt gaagctgcaa acaaaggctc tttgtcttgt gtcaaagagc atacctataa
601 gatcgagatg tgctataatc aggctttagg caaagtgaat gttctatctc ctaacagaaa
661 tgtccatgaa tggctgtaca gtttcaagcc aaatttcaat caagttgaaa gcaacaacag
721 aactgtaaat tctcttgcag tgaaatctct gctcatgtca gcagaaaaca acatcatgcc
781 taactctcaa gcttccactg attctcattt caagctgagc ctctggctaa gggttccaaa
841 ggttttgaag caggtttcca ttcagaaatt gttcaaggtt gcaggagatg aaacaaacaa
901 aacattttat ttatctattg cctgcattcc aaaccataac agtgttgaga cagctttaaa
961 cattactgtt atttgcaagc atcagctccc aattcgcaaa tgcaaagctc cttttgaatt
1021 atcaatgatg ttttctgatt taaaggagcc ttacaacatt gttcatgacc cttcataccc
1081 caaaggatcg gttccaatgc tctggctcga aactcacaca tctttgcaca agttctttgc
1141 aactaacttg caagaagatg taatcatcta cactttgaac aaccttgagc taactcctgg
1201 aaagttagat ttaggtgaaa gaaccttgaa ttacagtgaa gatgcctaca aaaggaaata
1261 tttcctttca aaaacacttg aatgtcttcc atctaacaca caaactatgt cttacttaga
1321 cagcatccaa atcccttcat ggaagataga ctttgccaga ggagaaatta aaatttctcc
1381 acaatctatt tcagttgcaa aatctttgtt aaagcttgat ttaagcggga tcaaaaagaa
1441 agaatctaag gttaaggaag cgtatgcttc aggatcaaaa taatcttgct ttgtccagct
1501 ttttctaatt atgttatgtt tattttcttt ctttacttat aattatttct ctgtttgtca
1561 tctctttcaa attcctcctg tctagtagaa accataaaaa caaaaaataa aaatgaaaat
1621 aaaattaaaa taaaataaaa tcaaaaaatg aaataaaaac aacaaaaaat taaaaaacga
1681 aaaaccaaaa agacccgaaa gggaccaatt tggccaaatt tgggttttgt ttttgttttt
1741 tgttttttgt tttttatttt ttattttatt tttattttat tttattttta ttttattttt
1801 attttattta ttttttgttt tcgttgtttt tgttatttta ttatttatta agcacaacac
1861 acagaaagca aactttaatt aaacacactt atttaaaatt taacacacta agcaagcaca
1921 agcaataaag ataaagaaag ctttatatat ttataggctt ttttataatt taacttacag
1981 ctgctttcaa gcaagttctg cgagttttgc ctgcttttta accccgaaca tttcatagaa
2041 cttgttaaga gtttcactgt aatgttccat agcaacactc cctttagcat taggattgct
2101 ggagctaagt atagcagcat actctttccc cttcttcacc tgatcttcat tcatttcaaa
2161 tgctttgctt ttcagcacag tgcaaacttt tcctaaggct tccttggtgt catacttctt
2221 tgggtcgatc ccgaggtcct tgtattttgc atcctgatat atagccaaga caacactgat
2281 catctcaaag ctatcaactg aagcaataag aggtaagcta cctcccagca ttatggcaag
2341 tctcacagac tttgcatcat cgagaggtaa tccataggct tgaatcaaag gatgggaagc
2401 aatcttagat ttgatagtat tgagattctc agaattccca gtttcttcaa caagcctgac
2461 cctgatcaag ctatcaagcc ttctgaaggt catgtcagtg cctccaatcc tgtctgaagt
2521 tttctttatg gtaattttac caaaagtaaa atcgctttgc ttaataacct tcattatgct
2581 ctgacgattc tttaggaatg tcagacatga aataacgctc atcttcttga tctggtcgat
2641 gttttccaga caaaaagtct tgaagttgaa tgctaccaga ttctgatctt cctcaaactc
2701 aaggtctttg ccttgtgtca acaaagcaac aatgctttcc ttagtgagct taaccttaga
2761 catgatgatc gtaaaagttg ttatatgctt tgaccgtatg taactcaagg tgcgaaagtg
2821 caactctgta tcccgcagtc gtttcttagg ttcttaatgt gatgatttgt aagactgagt
2881 gttaaggtat gaacacaaaa ttgacacgat tgctct
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