GenBank-Updates@genbank.bio.net (03/13/91)
LOCUS NPAPHDU 2400 bp ds-DNA VRL 13-MAR-1991
DEFINITION Autographa californica nuclear polyhedrosis virus, immediately
upstream region of the polyhedrin gene.
ACCESSION D00700 X06637
KEYWORDS polyhedrin gene; promoter.
SOURCE Autographa californica nuclear polyhedrosis virus C6 propagated in
Spodoptera frugiperda cells, genomic DNA.
ORGANISM Autographa californica nuclear polyhedrosis virus
Viridae; ds-DNA enveloped viruses; Baculoviridae; Baculovirus;
Nuclear polyhedrosis viruses.
REFERENCE 1 (bases 1978 to 2227)
AUTHORS Possee,R.D. and Howard,S.C.
TITLE Analysis of the polyhedrin gene promoter of the Autographa
californica nuclear polyhedrosis virus
JOURNAL Nucleic Acids Res. 15, 10233-10233 (1987)
STANDARD simple staff_entry
REFERENCE 2 (bases 1 to 2400)
AUTHORS Gearing,K.L. and Possee,R.D.
TITLE Functional analysis of a 603 nucleotide open reading frame upstream
of the polyhedrin gene of Autographa californica nuclear
polyhedrosis virus
JOURNAL J. Gen. Virol. 71, 251-262 (1990)
STANDARD full staff_review
COMMENT Deletion of the ORF 603 region did not affect virus replication in
cell culture.
FEATURES Location/Qualifiers
CDS complement(1426..2031)
/note="ORF 603"
repeat_region 2044..2051
/note="direct repeat"
repeat_region 2057..2064
/note="direct repeat"
misc_signal 2074..2084
/note="CAAT-like region"
misc_signal 2105..2114
/note="TATA-like region"
misc_feature 2135..2136
/note="mRNA start sites for ORF 603"
misc_feature 2138..2138
/note="mRNA start site for polyhedrin"
CDS 2187..>2400
/note="polyhedrin"
BASE COUNT 748 a 480 c 458 g 714 t
ORIGIN 3 bp upstream of XhoI site.
1 gagctagttc gttgacgcct tcctccgtgt ggccgaacac gtcgagcggg tggtcgatga
61 ccagcggcgt gccgccgcga cgcacaagta tctgtacacc gaatgatcgt cgggcgaagg
121 cacgtcggcc tccaagtggc aatattggca aattcgaaaa tatatacagt tgggttgttt
181 gcgcatatct atcgtggcgt tgggcatgta cgttcgaacg ttgatttgca tgcaagtcga
241 attaaatcat tgcgattagt gcgattaaaa cgttgtacat cctcgctttt aatcatgccg
301 tcgattaaat cgcgcaatcg agtcaagtga tcaaagtgtg gaataatgtt ttctttgtat
361 tcccgagtca agcgcagcgc gtattttaac aaactagcca tcttgtaagt tagtttcatt
421 taatgcaact ttatccaata atatattatg tatcgcacgt caagaattaa caatgcgccc
481 gttgtcgcat ctcaacacga ctatgataga gatcaaataa agcgcgaatt aaatagcttg
541 cgacgcaacg tgcacgatct gtgcacgcgt tccggcacga gctttgattg taataagttt
601 ttacgaagcg atgacatgac ccccgtagtg acaacgatca cgcccaaaag aactgccgac
661 tacaaaatta ccgagtatgt cggtgacgtt aaaactatta agccatccaa tcgaccgtta
721 gtcgaatcag gaccgctggt gcgagaagcc gcgaagtatg gcgaatgcat cgtataacgt
781 gtggagtccg ctcattagag cgtcatgttt agacaagaaa gctacatatt taattgatcc
841 cgatgatttt attgataaat tgaccctaac tccatacacg gtattctaca atggcggggt
901 tttggtcaaa atttccggac tgcgattgta catgctgtta acggctccgc ccactattaa
961 tgaaattaaa aattccaatt ttaaaaaacg cagcaagaga aacatttgta tgaaagaatg
1021 cgtagaagga aagaaaaatg tcgtcgacat gctgaacaac aagattaata tgcctccgtg
1081 tataaaaaaa atattgaacg atttgaaaga aaacaatgta ccgcgcggct gtgtacagga
1141 agaggtttat actaaactgt tacattgcaa acgtaatttc gtgtgccaag tgtgaaaacc
1201 gatgtttaat caaggctctg acgcatttct acaaccacga ctccaagtgt gtgggtgaag
1261 tcatgcatct tttaatcaaa tcccaagatg tgtataaacc accaaactgc caaaaaatga
1321 aaactgtcga caagctctgt tcgtttgctg gcaactgcaa gggctctaat cctatttgta
1381 attattgaat aataaaacaa ttataaatgc taaatttgtt ttttattaac gatacaaacc
1441 aaacgcaaca agaacatttg tagtattatc tataattgaa aacgcgtagt tataatcgct
1501 gaggtaatat ttaaaatcat tttcaaatga ttcacagtta atttgcgaca atataatttt
1561 attttcacat aaactagacg ccttgtcgtc ttcttcttcg tattccttct ctttttcatt
1621 tttctcctca taaaaattaa catagttatt atcgtatcca tatatgtatc tatcgtatag
1681 agtaaatttt ttgttgtcat aaatatatat gtctttttta atggggtgta tagtaccgct
1741 gcgactagtt tttctgtaat ttacaacagt gctattttct ggtagttctt cggagtgtgt
1801 tgctttaatt attaaattta tataatcaat gaatttggga tcgtcggttt tgtacaatat
1861 gttgccggca tagtacgcag cttcttctag ttcaattaca ccatttttta gcagcaccgg
1921 attaacataa ctttccaaaa tgttgtacga accgttaaac aaaaacagtt cacctccctt
1981 ttctatacta ttgtctgcga gcagttgttt gttgttaaaa ataacagcca ttgtaatgag
2041 acgcacaaac taatatcaca aactggaaat gtctatcaat atatagttgc tgatatcatg
2101 gagataatta aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca
2161 gttttgtaat aaaaaaacct ataaatatgc cggattattc ataccgtccc accatcgggc
2221 gtacctacgt gtacgacaac aagtactaca aaaatttagg tgccgttatc aagaacgcta
2281 agcgcaagaa gcacttcgcc gaacatgaga tcgaagaggc taccctcgac cccctagaca
2341 actacctagt ggctgaggat cctttcctgg gacccggcaa gaaccaaaaa ctcactctct
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