GenBank-Updates@genbank.bio.net (03/14/91)
LOCUS PSEIAAMH 3677 bp ds-DNA BCT 14-MAR-1991
DEFINITION P.syringae tryptophan 2-monooxygenase (iaaM) and indoleacetamide
hydrolase (iaaH) genes, complete cds.
ACCESSION M11035 M35690
KEYWORDS indoleacetamide hydrolase; indoleacetic acid;
tryptophan 2-monooxygenase; tryptophan-2-oxyreductase.
SOURCE P.syringae savastanoi (strain EW2009) DNA, clones pLUC2, pCP3, and
pCJP12.
ORGANISM Pseudomonas syringae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Pseudomonadaceae.
REFERENCE 1 (bases 202 to 3677)
AUTHORS Yamada,T., Palm,C.J., Brooks,B. and Kosuge,T.
TITLE Nucleotide sequences of the Pseudomonas savastanoi indoleacetic
acid genes show homology with agrobacterium tumefaciens T-DNA
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 82, 6522-6526 (1985)
STANDARD simple staff_review
REFERENCE 2 (bases 1 to 312)
AUTHORS Gaffney,T.D., da Costa e Silva,O., Yamada,T. and Kosuge,T.
TITLE Indoleacetic acid operon of Pseudomonas syringae subsp. savastanoi:
Transcription analysis and promoter identification
JOURNAL J. Bacteriol. 172, 5593-5601 (1990)
STANDARD simple staff_entry
COMMENT The iaaH and iaaM genes are part of an operon that is borne on a
plasmid, pIAA, in oleander strains of the pathogen. There appears
to be no genetic transformation of host tissue by P.savastanoi. No
Pribnow boxes were found, though potential ribosome binding sites
are located at positions 435-439 and 2164-2168. Expression of iaaH
depends upon the promoter for iaaM.
Draft entry and computer-readable sequence for [Proc. Natl. Acad.
Sci. U.S.A. 82, 6522-6526 (1985)] kindly submitted
by T.Yamada, 14-JAN-1986.
Draft entry and computer-readable sequence for [J. Bacteriol.
(1990) In press] kindly submitted
by T.D.Gaffney, 28-JUN-1990.
FEATURES Location/Qualifiers
misc_feature 19..47
/note="indoleacetic acid operon promoter"
mRNA 54..>3537
/note="indoleacetic acid mRNA"
CDS 82..312
/note="promoter proximal ORF"
/codon_start=82
CDS 454..2127
/note="tryptophan 2-monooxygenase (EC 1.13.12.3)"
/codon_start=454
misc_binding 577..651
/note="FAD binding site"
CDS 2170..3537
/note="indoleacetamide hydrolase"
/codon_start=2170
BASE COUNT 813 a 917 c 1046 g 901 t
ORIGIN 1 bp upstream of EcoRI site.
1 ccacaaaccg gcaagacgtt gaaaatcaat ttcaagcatt gtatgttttt ggtgggacgc
61 cacttggaaa atctggtgct gatgcccgga cggactcctg cgaactggct ggacaaggcg
121 atggtggcag tggcccgaat cagacagcgc aagccccagg cggcagtacc cgcggcgctc
181 ctccaagccc cgcacgctat ggaattcgtt tggagcacca ccaaaacctt taccgaatgc
241 cattgtccgg cttacacccc ctatacgcaa gccagtgcgt ggtcggccag cgcggtcgtt
301 agtgcttatt aattttagag gactagcatg gtttgaagga atggttcagg tgttttggat
361 tttggcggct gccgttaaaa ggtgctgttt cagtttcatt taaagaatcg taatccgggt
421 agcacgtaag gtcgaagagg gtagagtcgc gttatgtatg accattttaa ttcacccagt
481 attgatattt tgtacgacta cggtcccttt ctgaaaaaat gtgaaatgac gggaggcata
541 ggcagctatt cagccggaac gcccacccct cgggtagcga tagtcggtgc cggcatcagt
601 gggctggtcg ctgcaactga actattacgt gcgggagtca aggacgttgt cttatatgaa
661 tcgcgtgatc gaatcggggg acgggtatgg tctcaagttt tcgatcagac tcgtccacgt
721 tacattgcag aaatgggtgc gatgcgcttt cctcccagtg caactggcct tttccactac
781 ctgaaaaagt ttggtatttc gacgtcgacc acctttccgg atcctggtgt ggtggacacg
841 gagctgcatt accgtggcaa gcgctatcac tggccagcgg gcaaaaagcc gcccgaatta
901 ttcaggcgag tctatgaggg gtggcagtct ctattgtccg aaggttacct ccttgaaggc
961 ggttctttag ttgccccgct ggacattacc gcaatgctga agtcgggtcg tctggaagag
1021 gcagcgatcg catggcaggg atggctcaat gtattccggg attgttcatt ctataacgcg
1081 attgtctgta tttttactgg ccgccatccg ccaggcggcg acagatgggc tcgtcctgaa
1141 gactttgagc tgtttggctc gcttggcata ggctcgggcg ggtttttgcc agtctttcag
1201 gctggcttta cggaaatact gcggatggtt atcaacggat accaaagtga ccagcgactg
1261 attccggacg ggatatccag tctggccgcg agactcgctg atcagtcgtt tgacggcaaa
1321 gcgttaaggg accgcgtttg ttttagccgg gtaggtcgca tttccagaga agctgaaaaa
1381 atcatcatcc agacggaagc aggagaacag cgtgtatttg atcgagtaat tgtcactagc
1441 agtaatcggg ccatgcaaat gattcactgc ctcacggata gcgagagctt tctgagtcgt
1501 gatgtcgctc gtgctgtccg cgaaacccat ctgacaggtt catcgaagct tttcattctc
1561 acccgaacca aattctggat aaaaaacaag cttcccacca ccatccagtc ggacggtctg
1621 gtgcgcggcg tctattgtct ggattatcag cccgatgaac ctgaggggca tggcgttgtt
1681 ctgctcagtt acacgtggga agacgacgct caaaaaatgc tggcgatgcc tgacaagaaa
1741 acgcgttgcc aggtactggt tgatgacctt gctgcgatac acccgacgtt cgccagttat
1801 ctcctgcccg ttgatgggga ttatgagcgg tatgtattgc accatgactg gctcaccgat
1861 ccccattctg cgggcgcttt caaactcaat tatcccggcg aggacgttta ctcgcagcga
1921 ttgttttttc aaccaatgac agcgaacagt cccaataaag acacggggct ctatctggct
1981 ggctgcagtt gctcttttgc cggagggtgg atcgaaggtg ctgtccagac agcattgaac
2041 agtgcttgcg cggtgctgcg cagcaccgga gggcaactgt caaaaggcaa cccgctggac
2101 tgtatcaacg cctcctatcg ctattaacag tcctgagagc agcgctaagc taatacgggt
2161 gaaaagagca tgcatgaaat aatcacactt gaatcgctgt gccaggcatt ggctgatggc
2221 gagattgccg ctgcggagct gcgcgagcgt gcgctcgata ctgaggcccg cttggcccgt
2281 ttgaactgtt tcatacgtga gggtgatgcg gtcagccagt ttggcgaggc tgatcatgca
2341 atgaagggta ctccactctg gggaatgccg gtttccttca aggataatat ctgtgttcgc
2401 ggtttgccgt tgacagccgg gacgcgagga atgtccggtt tcgtatctga tcaggatgct
2461 gcgattgtca gtcaactcag agccctcggg gctgtggtcg ctggcaagaa caacatgcac
2521 gaactcagtt tcggagtgac ctccatcaat cctcattggg gaaccgtggg aaaccccgtg
2581 gcccccggtt attgtgccgg aggtagtagt ggtggaagtg ccgccgcagt ggcaagtgga
2641 attgttccgc tgtcggtggg gaccgacacg gggggctcga taagaatacc ggcggccttc
2701 tgcggcatta cgggcttcag acccactact ggacgctggt caacggcagg cattatccct
2761 gtttctcata caaaggattg cgttgggttg ctgacacgaa cggcaggcga cgccggattt
2821 ttgtacggac tgttgtcagg caagcagcag tcttttcccc tgagcaggac ggccccctgt
2881 cgaatcggcc taccggtctc catgtggtcc gatctggatg gcgaggtgga gagggcatgc
2941 gtgaatgcac tcagcctgct gcgcaagaca gggttcgagt ttattgaaat tgatgatgcc
3001 gatattgtcg aactgaacca gacactcacg ttcaccgttc cgctttacga attctttgcc
3061 gaccttgctc agtcattgct ctccttgggc tggaagcacg gtatccatca tatttttgca
3121 caggttgatg atgccaacgt gaaaggcatc atcaatcacc accttgggga gggcgctata
3181 aaaccggctc actatttgag ttcactgcaa aatggcgagc tgttgaaaag aaaaatggat
3241 gagctatttg ctcgtcataa tatcgagctc ctggggtacc ccacggtacc ttgccgggta
3301 cctcatctgg atcatgctga ccgaccagag tttttcagtc aggcaattcg caataccgac
3361 ctggccagca atgcgatgct cccctccatt actattcctg tcgggccaga agggcgattg
3421 ccggtcggct tgagctttga tgctctaaga gggcgagacg cacttttgct gagcagagtc
3481 agtgccatag agcaggtatt aggttttgta cgaaaagtat tgccgcatac aacgtaggct
3541 acaggccagc gtgaccatgg ctgcgtagct cttggccagc ttgtcgtagc gggtgccgat
3601 tcggcggttc tcttttagcc agccaaacat ccgctcaatg atgttgcgct gccgatactt
3661 tggacgatcg aagagtc
//